KEGG   ENZYME: 1.8.5.5
Entry
EC 1.8.5.5                  Enzyme                                 
Name
thiosulfate reductase (quinone);
phsABC (gene names)
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With a quinone or similar compound as acceptor
Sysname
sulfite,hydrogen sulfide:quinone oxidoreductase
Reaction(IUBMB)
sulfite + hydrogen sulfide + a quinone = thiosulfate + a quinol [RN:R11488]
Reaction(KEGG)
R11488 > R10149
Substrate
sulfite [CPD:C00094];
hydrogen sulfide [CPD:C00283];
quinone [CPD:C15602]
Product
thiosulfate [CPD:C00320];
quinol [CPD:C15603]
Comment
The enzyme, characterized from the bacterium Salmonella enterica, is similar to EC 1.17.5.3, formate dehydrogenase-N. It contains a molybdopterin-guanine dinucleotide, five [4Fe-4S] clusters and two heme b groups. The reaction occurs in vivo in the direction of thiosulfate disproportionation, which is highly endergonic. It is driven by the proton motive force that occurs across the cytoplasmic membrane.
History
EC 1.8.5.5 created 2016, modified 2017
Pathway
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08352  thiosulfate reductase / polysulfide reductase chain A
Genes
ECYECSE_P3-0024
STYSTY2271(phsA)
STTt0809(phsA)
SEXSTBHUCCB_8630
SENTTY21A_04105
STMSTM2065(phsA)
SEOSTM14_2555(phsA)
SEVSTMMW_20951
SEYSL1344_2041(phsA)
SEMSTMDT12_C20870
SEJSTMUK_2095(phsA)
SEBSTM474_2150(phsA)
SEFUMN798_2231(phsA)
SETUSTU288_06715
SETCCFSAN001921_06460
SENRSTMDT2_20381(phsA)
SENDDT104_21221(phsA)
SENICY43_11140
SEENSE451236_16535
SPQSPAB_01038
SEISPC_1649(phsA)
SECSCH_2074(phsA)
SEHSeHA_C2288
SHBSU5_02659
SENHCFSAN002069_21495
SEEHSEEH1578_19605
SEESNSL254_A2242
SENNSN31241_31660
SEWSeSA_A2235
SEASeAg_B2188
SENSQ786_10195
SEDSeD_A2401(phsA)
SEGSG2094(phsA)
SELSPUL_0835(phsA)
SEGASPUCDC_0835(phsA)
SETSEN2063(phsA)
SENAAU38_10410
SENOAU37_10420
SENVAU39_10420
SENQAU40_11665
SENLIY59_10705
SENJCFSAN001992_01110
SEECCFSAN002050_17285
SEEBSEEB0189_009140
SENBBN855_21500(phsA)
SENEIA1_10305
SENCSEET0819_17470
SESSARI_00822
SBGSBG_1889(phsA)
SBZA464_2189
SBVN643_09165
SALZEOS98_08685(phsA)
CFDCFNIH1_21320
CBRAA6J81_00990
CWECO701_07905
CYOCD187_09090
CPOTFOB25_20400(phsA)
CIFAL515_09925
CIEAN232_13865
CPARCUC49_05300
CITZE4Z61_02730(phsA)
CARSE1B03_16850(phsA)
PMRPMI1814(phsA)
PMIBBB2000_1921(phsA)
PVLAOB99_11090
PVGCRN77_09800
PHAUPH4a_01215
PROTBTA34_10765
PCOLF1325_12680(phsA)
PCIBF9282_12780(phsA)
PPEEI6G31_15405(phsA)
EICNT01EI_1854
ETRETAE_1845(phsA)
ETDETAF_1665
ETEETEE_3891
ETCETAC_08455
EDWQY76_11045
EDLAAZ33_09395(phsA)
EHOA9798_08680
LRINCTC12151_02197(psrA)
PGBH744_2c2539
PLULFOB45_15630
MARAD0851_14340
SONSO_4062(phsA)
SFRSfri_1999
SAZSama_3177
SBMShew185_0531
SBNSbal195_0555
SBPSbal223_0559
SBTSbal678_0563
SBBSbal175_0622
SLOShew_0608
SHPSput200_0542
SSESsed_4047
SPLSpea_3039 Spea_3188
SHEShewmr4_3444
SHMShewmr7_0507
SHNShewana3_3619
SHLShal_3127 Shal_3271
SWPswp_1238
SHFCEQ32_19375
SJASJ2017_3760
SBJCF168_02695
SMAVCFF01_02980
SHEWCKQ84_11085
SALGBS332_04285
SLJEGC82_11685(phsA)
SMAIEXU30_13320(phsA)
SPOLFH971_10120(phsA)
SKHSTH12_04225(psrA)
SLITJQC75_16275(phsA)
SCAATUM17387_33610(phsA)
SPSHFM037_24400(phsA)
SEURFM038_002915(phsA)
SYKKDN34_15170(phsA)
SAVIJYB87_15740(phsA)
FBLFbal_3636
FESHER31_12320(phsA)
MDNJT25_008470
TUNJ9260_10030
TLOJ9253_04310
TSBHMY34_18450
TANIJ8380_14995
TTPE6P07_03930
CJAPGWK36_03095
AEHMlg_0633 Mlg_2426
HHAHhal_1166
EBSECTOBSL9_2837(arxA)
TNITVNIR_0949(phsA_[H])
TTITHITH_03005
NAXHC341_06630
HCOLOKO_02585(psrA)
KUSB9G99_05350
KMAB9H00_09830
SEDSAAY24_06365 AAY24_12230
MASSCR152_08555
MFLAGO485_03455
MPLIE1742_13795
SUTTSUTMEG_11480 SUTMEG_15000
SUTKFG381_02115
SWSI6J16_05350
BBAYA4V04_07945
SHDSUTH_00426
SLACSKTS_23020
ARESIWH25_01975
EBAebA1017
RBHB4966_04570 B4966_04605
AZAAZKH_p0053
AZIAzCIB_1147
ACOMCEW83_03250
HWIA0Z60_06255(phsA)
WSUWS0116(fdhA) WS0709(fdhA)
SUASaut_0756 Saut_0759
SULNFJR47_09570
SULCCVO_09640
CCVCCV52592_1914
CCOCCC13826_0389
CCOCCCON33237_1350
CAJCIG1485E_0644
CSMCSUB8521_1325
CGRACGRAC_0560
CURECUREO_1061
CHYOCHH_0519
CHVCHELV3228_0175
CSPFCSF_0471
CPINCPIN18020_0457
CRXCRECT_0711
CGEOCGEO_1111
CBLACBLAS_1021
CCORCCORG_0662
CMUCCMCT_0504
CSHOCSHOW_1391
CNVCNZW441b_1003
SDLSdel_0265 Sdel_0269 Sdel_2276
SBASulba_0281 Sulba_0285 Sulba_2546
SMULSMUL_0342(psrA) SMUL_0346 SMUL_3275 SMUL_3282
SHALSHALO_0307 SHALO_0311 SHALO_2994 SHALO_3001
SULSSdiek1_0301 Sdiek1_0305 Sdiek1_2974 Sdiek1_2981
SULJSJPD1_0305 SJPD1_0309 SJPD1_2864 SJPD1_2871
SULTFA592_07095 FA592_08605(phsA) FA592_08625(phsA)
AHSAHALO_0201 AHALO_2400
AMYTAMYT_1905
AMARAMRN_0240 AMRN_2501
ACAAACAN_0252 ACAN_2514
AMOLAMOL_1120 AMOL_1900
HEBRAEBR_0305 AEBR_1184
AANAAANAER_0294
NSANitsa_1756
SINUIMZ28_06785 IMZ28_07170 IMZ28_07270
NAMNAMH_1518
NAPC3L23_07885
CMEDFE773_08475
CPAFC6V80_01970
GLOGlov_1164
PEFA7E78_03700
DESDSOUD_0599
DEUDBW_0837
DVEDESUT3_07870
DVUDVU_0173
DVLDvul_2794
DVMDvMF_0166 DvMF_0320
DVGDeval_0198
DDSDdes_1667
DPGDESPIGER_0221
DEFCNY67_04550
DFLDFE_0077 DFE_0136
DSDGD606_10630 GD606_17415
DMADMR_08220(phaA)
DCBC3Y92_16355
DGGDGI_0241
DDEDde_0682 Dde_1076
DMSE8L03_06385
DSADesal_0371
DAFDesaf_1489 Desaf_1628 Desaf_3550
DASDaes_1184 Daes_3179
DPIBN4_20079
DEJAWY79_01170 AWY79_07465
PPRFDPRO_1730 DPRO_3417
PSELGM415_04510 GM415_09000 GM415_09450
DDNDND132_0424 DND132_1314 DND132_2597
DSXGD604_01660 GD604_16610
PBIZLWC08_08725
DBADbac_0171 Dbac_2338 Dbac_2860
DRTDret_0895
DSFUWK_01205 UWK_02642 UWK_02791
DAKDaAHT2_0420 DaAHT2_0547
DPRDespr_1135 Despr_1859 Despr_2399
DOGHP555_09720 HP555_14005
DBKDGMP_14050 DGMP_21170 DGMP_32000
DOLDole_0126
DMLDmul_02650(phsA)
DALDalk_1125 Dalk_2525
DATHRM2_14230
DTOTOL2_C03980(phsA)
DOVDSCO28_04200 DSCO28_16650 DSCO28_54490
DWDDSCW_04650 DSCW_28610 DSCW_31190
DALKDSCA_26800 DSCA_49610 DSCA_57040 DSCA_61680
DMMdnm_034970
ADEAdeh_2632
ACPA2cp1_2813
AFWAnae109_4110
ANKAnaeK_2718
SATSYN_01672
DTIDesti_1387 Desti_1738
SFUSfum_1318 Sfum_3287
DAXFDQ92_10105
DBRDeba_2842
DAVDESACE_06425
DHRLGS26_06635 LGS26_09210
ACRAcry_0345
AMVACMV_03760(psrA)
HTLHPTL_0252
BBEVBBEV_1018(psrA-1)
BSEBsel_1476
CCECcel_1634
STHSTH109
DSYDSY3896
DHDDhaf_1509 Dhaf_1886
DDHDesde_1410
DDLDesdi_1252 Desdi_2963
DMTDESME_00815 DESME_05785
DORDesor_3577
DAIDesaci_3271
DCADesca_0977
DKUDesku_2752
AACXDEACI_1081 DEACI_3149
DGIDesgi_1689
CTHMCFE_0713 CFE_1029
CHYCHY_2574
MTAMoth_0450
MTHOMOTHE_c03860(psrA)
MTHZMOTHA_c04750(psrA)
TNRThena_1758
TACITDSAC_1714
LPILLIP_1347
SPACB1H29_02555
CCUCcur_02090
ELEElen_0442 Elen_0447 Elen_2753 Elen_2919
EYYEGYY_22930
EGDGS424_002570 GS424_002595 GS424_016830
GPAGPA_25490
AEQAEQU_0172 AEQU_0395
AHATADCFC_03680 ADCFC_20030
DDTAAY81_08500
CBACJI75_05060 JI75_08700
RTSCE91St31_28640 CE91St31_29860
CAERCSV91_01930
CAPCLDAP_25520(phaA)
THUGKNN16_09725
TTHTT_C0155
TSCTSC_c06950
THCTCCBUS3UF1_7880
TOSTheos_0751
TBCA0O31_01685
OPROcepr_1002
OTEOter_1466
DRCG0Q07_19410
MCOSGM418_06250
MBASALGA_3219
FBCFB2170_11556
LUTLupro_00130
SABUMBM09_11180
SHYRLA303_10780
CCHCag_0620
CPHCpha266_2562
CPBCphamn1_2386
CLIClim_2400
PPHPpha_0912
PAAPaes_2139
PROCPtc2401_02170(psrA)
PRSB9H02_10550
PROSCHL67_11175
IALIALB_1663
MROMROS_1774
DINSelin_2609
DDFDEFDS_0670 DEFDS_1699
DAPDacet_1678 Dacet_1793
GTLEP073_09415 EP073_11320
CNICalni_0185
FSIFlexsi_1504 Flexsi_1512
CABYCabys_1269
TYETHEYE_A1128 THEYE_A1157
TIDThein_1622
TOPTOPB45_0897
TCMHL41_04470 HL41_08650
THETF1847_03060 F1847_07855
CTHITHC_0921
TAVG4V39_07125 G4V39_08920
TMAIFVE67_02275
HALHHTSR_1661
HHSRHSR6_1729
HSUHLASF_1287
HSFHLASA_1275
HARAAArcS_0641(bisC) AArcS_0990(bisC2)
HSNDV733_07300
HDSHSR122_1817(bisC) HSR122_2527(bisC2) HSR122_2992(bisC4)
HDFAArcSl_0127(phsA) AArcSl_1905(phsA2)
NGENatgr_0551 Natgr_2276
SAIMK0C01_00030
NAGAArcMg_2662
NANAArc1_1042
PFMPyrfu_1082
 » show all
Reference
1  [PMID:6384182]
  Authors
Kwan HS, Barrett EL
  Title
Map locations and functions of Salmonella typhimurium men genes.
  Journal
J Bacteriol 159:1090-2 (1984)
DOI:10.1128/JB.159.3.1090-1092.1984
Reference
2  [PMID:3108233]
  Authors
Clark MA, Barrett EL
  Title
The phs gene and hydrogen sulfide production by Salmonella typhimurium.
  Journal
J Bacteriol 169:2391-7 (1987)
DOI:10.1128/JB.169.6.2391-2397.1987
Reference
3  [PMID:7737516]
  Authors
Alami N, Hallenbeck PC
  Title
Cloning and characterization of a gene cluster, phsBCDEF, necessary for the production of hydrogen sulfide from thiosulfate by Salmonella typhimurium.
  Journal
Gene 156:53-7 (1995)
DOI:10.1016/0378-1119(94)00930-Q
  Sequence
Reference
4  [PMID:7751291]
  Authors
Heinzinger NK, Fujimoto SY, Clark MA, Moreno MS, Barrett EL
  Title
Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism.
  Journal
J Bacteriol 177:2813-20 (1995)
DOI:10.1128/JB.177.10.2813-2820.1995
  Sequence
Reference
5  [PMID:22081391]
  Authors
Stoffels L, Krehenbrink M, Berks BC, Unden G
  Title
Thiosulfate reduction in Salmonella enterica is driven by the proton motive force.
  Journal
J Bacteriol 194:475-85 (2012)
DOI:10.1128/JB.06014-11
Other DBs
ExplorEnz - The Enzyme Database: 1.8.5.5
IUBMB Enzyme Nomenclature: 1.8.5.5
ExPASy - ENZYME nomenclature database: 1.8.5.5
BRENDA, the Enzyme Database: 1.8.5.5
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