KEGG ENZYME: 2.1.1.212

ENZYME: 2.1.1.212

Entry

EC 2.1.1.212                Enzyme

Name

2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase;
SAM:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase;
HI4'OMT;
HMM1;
MtIOMT5;
S-adenosyl-L-methionine:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:2,4',7-trihydroxyisoflavanone 4'-O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + 2,4',7-trihydroxyisoflavanone = S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone [RN:R07722]

Reaction(KEGG)

R07722

Substrate

S-adenosyl-L-methionine [CPD:C00019];
2,4',7-trihydroxyisoflavanone [CPD:C15567]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
2,7-dihydroxy-4'-methoxyisoflavanone [CPD:C16190]

Comment

Specifically methylates 2,4',7-trihydroxyisoflavanone on the 4'-position. No activity with isoflavones [2]. The enzyme is involved in formononetin biosynthesis in legumes [1]. The protein from pea (Pisum sativum) also methylates (+)-6a-hydroxymaackiain at the 3-position (cf. EC 2.1.1.270, (+)-6a-hydroxymaackiain 3-O-methyltransferase) [4].

History

EC 2.1.1.212 created 2011

Pathway

ec00943

Isoflavonoid biosynthesis

Orthology

K13259

2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase / isoflavone 4'-O-methyltransferase

Genes

GMX:	100807589(IOMT1) 106795493(IOMT2)

GSJ:	114374101 114374126

PVU:

137805790

VRA:

106780380

VAR:

108341659

VUN:

114175643

VUM:	124837707 124845747

CCAJ:

109816528

APRC:

113858334

MTR:

25493072

TPRA:

123881701 123913950 123913951

CAM:	101494507 101494823 101495151

PSAT:

127108027

LJA:	130711100 130711801

ADU:

107480758

AIP:

107631191

AHF:	112733553 112791519

LANG:

109349376

» show all

Reference

1 [PMID:12610212]

Authors

Akashi T, Sawada Y, Shimada N, Sakurai N, Aoki T, Ayabe S

Title

cDNA cloning and biochemical characterization of S-adenosyl-L-methionine: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway.

Journal

Plant Cell Physiol 44:103-12 (2003)
DOI:10.1093/pcp/pcg034

Sequence

[ag:BAC58011 BAC58013]

Reference

2 [PMID:17001495]

Authors

Deavours BE, Liu CJ, Naoumkina MA, Tang Y, Farag MA, Sumner LW, Noel JP, Dixon RA

Title

Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula.

Journal

Plant Mol Biol 62:715-33 (2006)
DOI:10.1007/s11103-006-9050-x