KEGG   ENZYME: 2.1.1.249
Entry
EC 2.1.1.249                Enzyme                                 

Name
dimethylamine---corrinoid protein Co-methyltransferase;
mtbB (gene name);
dimethylamine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
dimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] = a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine [RN:R10015]
Reaction(KEGG)
R10015;
(other) R09999
Substrate
dimethylamine [CPD:C00543];
[Co(I) dimethylamine-specific corrinoid protein]
Product
[methyl-Co(III) dimethylamine-specific corrinoid protein];
methylamine [CPD:C00218]
Comment
The enzyme, which catalyses the transfer of a methyl group from dimethylamine to a dimethylamine-specific corrinoid protein (MtbC), is involved in methanogenesis from dimethylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
History
EC 2.1.1.249 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K16178  dimethylamine---corrinoid protein Co-methyltransferase
Genes
FWA: DCMF_06200 DCMF_16340 DCMF_21085 DCMF_23055 DCMF_25380 DCMF_25995 DCMF_28300
AMIC: Ami3637_11500
TPZ: Tph_c27720(mtbB2)
AAR: Acear_1273
MBY: MSBRM_0457 MSBRM_0926 MSBRM_0957
MBW: MSBRW_1129 MSBRW_2236 MSBRW_3223
MBAR: MSBR2_1469 MSBR2_1924
MBAK: MSBR3_0479 MSBR3_0618 MSBR3_1955
MAC: MA_0532(mtbB) MA_0933(mtbB) MA_2425(mtbB)
MMA: MM_1693(mtbB) MM_2051(mtbB) MM_2962(mtbB)
MPY: Mpsy_1680(mtbB) Mpsy_1702(mtbB3)
MARC: AR505_1332
 » show all
Reference
1  [PMID:9654067]
  Authors
Wassenaar RW, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and characterization of dimethylamine:5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro.
  Journal
Eur J Biochem 253:692-7 (1998)
DOI:10.1046/j.1432-1327.1998.2530692.x
Reference
2  [PMID:10852929]
  Authors
Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA
  Title
Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199
  Sequence
Reference
3  [PMID:15450490]
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.249
IUBMB Enzyme Nomenclature: 2.1.1.249
ExPASy - ENZYME nomenclature database: 2.1.1.249
BRENDA, the Enzyme Database: 2.1.1.249
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