KEGG ENZYME: 2.1.1.249

ENZYME: 2.1.1.249

Entry

EC 2.1.1.249                Enzyme

Name

dimethylamine---corrinoid protein Co-methyltransferase;
mtbB (gene name);
dimethylamine methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

dimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase

Reaction(IUBMB)

dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] = a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine [RN:R10015]

Reaction(KEGG)

R10015;
(other) R09999

Substrate

dimethylamine [CPD:C00543];
[Co(I) dimethylamine-specific corrinoid protein]

Product

[methyl-Co(III) dimethylamine-specific corrinoid protein];
methylamine [CPD:C00218]

Comment

The enzyme, which catalyses the transfer of a methyl group from dimethylamine to a dimethylamine-specific corrinoid protein (MtbC), is involved in methanogenesis from dimethylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.

History

EC 2.1.1.249 created 2012

Pathway

ec00680

Methane metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K16178

dimethylamine---corrinoid protein Co-methyltransferase

Genes

FWA:	DCMF_06200 DCMF_16340 DCMF_21085 DCMF_23055 DCMF_25380 DCMF_25995 DCMF_28300

AMIC:

Ami3637_11500

ETM:	CE91St48_13070(mtbB_1)

TPZ:	Tph_c27720(mtbB2)

AAR:	Acear_1273

MBY:	MSBRM_0457 MSBRM_0926 MSBRM_0957

MBW:	MSBRW_1129 MSBRW_2236 MSBRW_3223

MBAR:

MSBR2_1469 MSBR2_1924

MBAK:

MSBR3_0479 MSBR3_0618 MSBR3_1955

MAC:	MA_0532(mtbB) MA_0933(mtbB) MA_2425(mtbB)

MMA:	MM_1693(mtbB) MM_2051(mtbB) MM_2962(mtbB)

MMJ:	MSMAS_1746 MSMAS_2635 MSMAS_2980

MMAC:

MSMAC_1375 MSMAC_1454 MSMAC_1786

MVC:	MSVAZ_0482 MSVAZ_1227 MSVAZ_1897

MEK:	MSKOL_0466 MSKOL_1170 MSKOL_1883

MLS:	MSLAZ_0292 MSLAZ_1667 MSLAZ_2478

METM:

MSMTP_1884 MSMTP_2549 MSMTP_2907

MEF:	MSWH1_0342 MSWH1_1227 MSWH1_1620 MSWH1_2498

MEQ:	MSWHS_0354 MSWHS_1304 MSWHS_1681 MSWHS_2546

MSJ:	MSSAC_0463 MSSAC_1562 MSSAC_2376

MSZ:	MSSIH_0432 MSSIH_1412 MSSIH_1979

MSW:	MSSIT_0457 MSSIT_1463 MSSIT_2023

MTHR:

MSTHT_0489 MSTHT_1361 MSTHT_2067

MTHE:

MSTHC_0232 MSTHC_1217 MSTHC_1931

MHOR:

MSHOH_1542 MSHOH_2132 MSHOH_3685

MMET:

MCMEM_0438 MCMEM_1855

MHAZ:

BHR79_00930 BHR79_05340

MEV:	Metev_1439 Metev_1945

MZH:	Mzhil_0605

MPY:	Mpsy_1680(mtbB) Mpsy_1702(mtbB3)

MHZ:	Metho_0033 Metho_1026

TAR:	TALC_00301 TALC_01103

MAX:	MMALV_11370

MER:	MMINT_09020

MARC:

AR505_1332

» show all

Reference

1 [PMID:9654067]

Authors

Wassenaar RW, Keltjens JT, van der Drift C, Vogels GD

Title

Purification and characterization of dimethylamine:5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro.

Journal

Eur J Biochem 253:692-7 (1998)
DOI:10.1046/j.1432-1327.1998.2530692.x

Reference

2 [PMID:10852929]

Authors

Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA

Title

Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.

Journal

J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199

Sequence

[ag:AAD14633]

Reference

3 [PMID:15450490]

Authors

Krzycki JA

Title

Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.

Journal

Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012