KEGG ENZYME: 2.1.1.341

ENZYME: 2.1.1.341

Entry

EC 2.1.1.341                Enzyme

Name

vanillate/3-O-methylgallate O-demethylase;
ligM (gene name)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

vanillate:tetrahydrofolate O-methyltransferase

Reaction(IUBMB)

(1) vanillate + tetrahydrofolate = protocatechuate + 5-methyltetrahydrofolate [RN:R10136];
(2) 3-O-methylgallate + tetrahydrofolate = gallate + 5-methyltetrahydrofolate [RN:R09271]

Reaction(KEGG)

R09271 R10136

Substrate

vanillate [CPD:C06672];
tetrahydrofolate [CPD:C00101];
3-O-methylgallate [CPD:C05616]

Product

protocatechuate [CPD:C00230];
5-methyltetrahydrofolate [CPD:C00440];
gallate [CPD:C01424]

Comment

The enzyme, characterized from the bacterium Sphingomonas sp. SYK6, is involved in the degradation of lignin. The enzyme has similar activities with vanillate and 3-O-methylgallate.

History

EC 2.1.1.341 created 2017

Pathway

ec00627

Aminobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K15066

vanillate/3-O-methylgallate O-demethylase

Genes

CDN:	BN940_04261

MAMO:

A6B35_30230

MHUA:

MCHK_13105

NIY:	FQ775_18790

NTU:	NTH_01138(gcvT_2) NTH_01148(gcvT_3)

MESO:

BSQ44_07725 BSQ44_08460

CHEA:

PVE73_01565 PVE73_05425

ORM:	HTY61_01840

ATU:	Atu1420(gcvT)

ALF:	CFBP5473_15970

AVQ:	HRR99_21670

AVV:	RvVAT039_39160(gcvT)

AVF:	RvVAR031_42080(gcvT)

ASAL:

CFBP5507_05440

RIR:	BN877_p0379

ALEG:

CFBP4996_19965

AGRC:

GSF67_05790

AVI:	Avi_5560(gcvT)

RLE:

pRL90255

RLT:	Rleg2_6553

RAD:	CO657_33455

RAW:	NE851_32395

RHIZ:

QMO80_006283

NGL:	RG1141_CH21810 RG1141_CH37800

NGG:	RG540_CH22690 RG540_CH38450 RG540_PA15060

NEO:	CYG48_08640 CYG48_14345

NPM:	QEO92_11585 QEO92_22220

RHT:	NT26_2004(gcvT)

RFV:	RFYW14_02079

BVV:	BHK69_29845

BVES:

QO058_29400

BOSE:

RMR04_00430

DEA:	FPZ08_20670

MAAD:

AZF01_20325

MLUT:

JET14_00525

PSIN:

CAK95_06130 CAK95_28490

CAUL:

KCG34_07165

MALU:

KU6B_14370(gcvT_2)

RMAI:

MACH21_12550(gcvT_2)

PTP:	RCA23_c08390(gcvT1)

SALO:

EF888_01245

PSHQ:

F3W81_00420

NSM:	JO391_18175

PBAE:

P8S53_03180

RBG:	BG454_07655

MANH:

LA6_004263(gcvT_11)

NAR:

Saro_2861

NPP:	PP1Y_AT11476

NOT:	C7W88_07710

NOR:	FA702_09220

NDR:	HT578_07580

NHUM:

PQ457_02295

NCP:	U0041_18125

NOVO:

IM701_13020

RDI:	CMV14_03765 CMV14_12635 CMV14_16120

SPHI:

TS85_14190

SPHC:

CVN68_17190

SGLC:

M1K48_07695

SJP:	SJA_C2-01210

SCH:	Sphch_4032

SSY:	SLG_12740(ligM)

SUFL:

FIL70_20895

SBAR:

H5V43_17545

SPCA:

GL174_14870 GL174_15045 GL174_19040

SPHG:

AZE99_04695

ACOB:

P0Y56_11965

ALB:	AEB_P0223 AEB_P2703

AAY:	WYH_01929(gcvT_1)

TRO:	trd_0424 trd_0901 trd_A0792

STI:	Sthe_0208 Sthe_0782 Sthe_1118 Sthe_2713 Sthe_2797

OLE:	K0B96_04355

MICA:

P0L94_12945 P0L94_12985 P0L94_13025 P0L94_13135 P0L94_13190 P0L94_16895

SVL:	Strvi_6107

SSOI:

I1A49_02875 I1A49_02900

SBH:

SBI_08592

SAUO:

BV401_03150 BV401_03175

SDX:	C4B68_00235

SNQ:	CP978_01440

SSPN:

LXH13_37930

KBU:	Q4V64_39625

STRL:

HEP84_03085

STSD:

HEP87_04495

SRPZ:

HEP85_37280

AREV:

RVR_1588 RVR_396 RVR_642

MLV:	CVS47_03107(dmdA) CVS47_03109(gcvT_3) CVS47_03110(gcvT_4) CVS47_03141(gcvT_5)

MWA:	E4K62_02375 E4K62_17420 E4K62_17600 E4K62_18420

MPRT:

ET475_01815 ET475_04585

MSED:

E3O41_03730 E3O41_12915

MSF:	IT882_03335 IT882_03345 IT882_03355 IT882_14810

MCAW:

F6J84_14240 F6J84_14285 F6J84_14310 F6J84_14320

MLZ:	F6J85_02175 F6J85_16505 F6J85_16690 F6J85_17425

MBIN:

LXM64_14925 LXM64_14970 LXM64_15040 LXM64_15055

MELY:

L2X98_21655 L2X98_24855 L2X98_30345 L2X98_30375 L2X98_30535

MINV:

T9R20_15950 T9R20_15990 T9R20_16020

MLIY:

RYJ27_00245 RYJ27_09240 RYJ27_09250 RYJ27_09260 RYJ27_12580

MICE:

EHF32_13620 EHF32_13640 EHF32_13720 EHF32_13765

MHOT:

PU630_12155 PU630_12175 PU630_14575 PU630_14650

MICI:

AB2L57_13805 AB2L57_13815 AB2L57_13835 AB2L57_16645

LALL:

MUN78_00550 MUN78_00610 MUN78_00755

NAEI:

GCM126_21180(gcvT_2) GCM126_21270 GCM126_21300

ACRY:

AC20117_11110

AJG:	KKR91_03955 KKR91_04000

ASUF:

MNQ99_02495 MNQ99_16860

ACAO:

NF551_03905 NF551_03950

AGEG:

MUG94_03705 MUG94_03760

AKO:	N9A08_04120

AZX:	N2K95_03755 N2K95_03800

AJR:	N2K98_03835 N2K98_03875

PSNI:

NIBR502771_05695 NIBR502771_05725 NIBR502771_05730

PDEL:

JCQ34_19080 JCQ34_19110

KRS:	EQG70_16155

MTEU:

R3I42_05970

SATK:

SA2016_1316 SA2016_1317 SA2016_1323

CCYC:

SCMU_11410(gcvT_2) SCMU_11420(gcvT_3) SCMU_11450(gcvT_4)

NAE:	BHE16_10075

CIG:	E7744_01440 E7744_01750

CMUA:

P8192_00400 P8192_03060 P8192_13290

BKI:	M4486_03585

BLIN:

BLSMQ_1396

BCAU:

I6G59_01325

BREB:

PGC08_07175

NOCL:

KM427_16055

ACTQ:

OG417_09410 OG417_21095

BSD:	BLASA_2600(gcvT2) BLASA_2601(gcvT3)

BBRG:

U6N30_09160 U6N30_09165

MMAR:

MODMU_2841(gcvT) MODMU_2842(gcvT)

SVI:	Svir_11650 Svir_33030

AMQ:	AMETH_2824

AACD:

LWP59_22725 LWP59_22730

ARHD:

VSH64_16020 VSH64_18850 VSH64_22985 VSH64_45245

AMYD:

K1T34_38880 K1T34_48055

ABAR:

AMYBAR_002610 AMYBAR_002612

PDX:

Psed_2464

PSEA:

WY02_25075

PAUT:

Pdca_48160(gcvT_2)

PSDS:

K1T35_20825 K1T35_21645 K1T35_34350 K1T35_34365

ACTL:

L3i22_065780(gcvT)

RXY:	Rxyl_0192 Rxyl_0209 Rxyl_1565

HDO:	MUK72_18085

HLM:	DV707_07675 DV707_09365 DV707_10535 DV707_15670 DV707_15690 DV707_16580 DV707_16605 DV707_16610 DV707_16920 DV707_17025

HDF:	AArcSl_0610(ligM)

HLR:	HALLA_02090

» show all

Reference

1 [PMID:9501423]

Authors

Nishikawa S, Sonoki T, Kasahara T, Obi T, Kubota S, Kawai S, Morohoshi N, Katayama Y

Title

Cloning and sequencing of the Sphingomonas (Pseudomonas) paucimobilis gene essential for the O demethylation of vanillate and syringate.

Journal

Appl Environ Microbiol 64:836-42 (1998)
DOI:10.1128/AEM.64.3.836-842.1998

Reference

2 [PMID:15090517]

Authors

Masai E, Sasaki M, Minakawa Y, Abe T, Sonoki T, Miyauchi K, Katayama Y, Fukuda M

Title

A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate.

Journal

J Bacteriol 186:2757-65 (2004)
DOI:10.1128/jb.186.9.2757-2765.2004

Reference

3 [PMID:15743951]

Authors

Abe T, Masai E, Miyauchi K, Katayama Y, Fukuda M

Title

A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6.

Journal

J Bacteriol 187:2030-7 (2005)
DOI:10.1128/JB.187.6.2030-2037.2005

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.341

IUBMB Enzyme Nomenclature:

2.1.1.341

ExPASy - ENZYME nomenclature database:

2.1.1.341

BRENDA, the Enzyme Database:

2.1.1.341

LinkDB

DBGET integrated database retrieval system