KEGG   ENZYME: 2.1.1.341
Entry
EC 2.1.1.341                Enzyme                                 
Name
vanillate/3-O-methylgallate O-demethylase;
ligM (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
vanillate:tetrahydrofolate O-methyltransferase
Reaction(IUBMB)
(1) vanillate + tetrahydrofolate = protocatechuate + 5-methyltetrahydrofolate [RN:R10136];
(2) 3-O-methylgallate + tetrahydrofolate = gallate + 5-methyltetrahydrofolate [RN:R09271]
Reaction(KEGG)
R09271 R10136
Substrate
vanillate [CPD:C06672];
tetrahydrofolate [CPD:C00101];
3-O-methylgallate [CPD:C05616]
Product
protocatechuate [CPD:C00230];
5-methyltetrahydrofolate [CPD:C00440];
gallate [CPD:C01424]
Comment
The enzyme, characterized from the bacterium Sphingomonas sp. SYK6, is involved in the degradation of lignin. The enzyme has similar activities with vanillate and 3-O-methylgallate.
History
EC 2.1.1.341 created 2017
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K15066  vanillate/3-O-methylgallate O-demethylase
Genes
CDNBN940_04261
MAMOA6B35_30230
MHUAMCHK_13105
MESOBSQ44_07725 BSQ44_08460
NIYFQ775_18790
ORMHTY61_01840
ATUAtu1420(gcvT)
AVIAvi_5560(gcvT)
ALFCFBP5473_15970
RLEpRL90255
RLTRleg2_6553
RIRBN877_p0379
RADCO657_33455
NGLRG1141_CH21810 RG1141_CH37800
NGGRG540_CH22690 RG540_CH38450 RG540_PA15060
NEOCYG48_08640 CYG48_14345
RHTNT26_2004(gcvT)
RFVRFYW14_02079
BVVBHK69_29845
DEAFPZ08_20670
MAADAZF01_20325
MLUTJET14_00525
PSINCAK95_06130 CAK95_28490
CAULKCG34_07165
MALUKU6B_14370(gcvT_2)
PTPRCA23_c08390(gcvT1)
RBGBG454_07655
SALOEF888_01245
PSHQF3W81_00420
NARSaro_2861
NPPPP1Y_AT11476
NOTC7W88_07710
NORFA702_09220
NDRHT578_07580
RDICMV14_03765 CMV14_12635 CMV14_16120
SPHITS85_14190
SPHCCVN68_17190
SJPSJA_C2-01210
SCHSphch_4032
SSYSLG_12740(ligM)
SUFLFIL70_20895
SBARH5V43_17545
SPHGAZE99_04695
ALBAEB_P0223 AEB_P2703
AAYWYH_01929(gcvT_1)
CCYCSCMU_11410(gcvT_2) SCMU_11420(gcvT_3) SCMU_11450(gcvT_4)
SVLStrvi_6107
SSOII1A49_02900
SBHSBI_08592
SAUOBV401_03150 BV401_03175
SDXC4B68_00235
SNQCP978_01440
MLVCVS47_03107(dmdA) CVS47_03109(gcvT_3) CVS47_03110(gcvT_4) CVS47_03141(gcvT_5)
MWAE4K62_02375 E4K62_17420 E4K62_17600 E4K62_18420
MPRTET475_01815 ET475_04585
MSEDE3O41_03730 E3O41_12915
MSFIT882_03335 IT882_03345 IT882_03355 IT882_14810
MCAWF6J84_14240 F6J84_14285 F6J84_14310 F6J84_14320
MLZF6J85_02175 F6J85_16505 F6J85_16690 F6J85_17425
ACRYAC20117_11110
AJGKKR91_03955 KKR91_04000
PSNINIBR502771_05695 NIBR502771_05725 NIBR502771_05730
KRSEQG70_16155
SATKSA2016_1316 SA2016_1317 SA2016_1323
NAEBHE16_10075
CIGE7744_01440 E7744_01750
BLINBLSMQ_1396
BCAUI6G59_01325
BSDBLASA_2600(gcvT2) BLASA_2601(gcvT3)
MMARMODMU_2841(gcvT) MODMU_2842(gcvT)
SVISvir_11650 Svir_33030
AMQAMETH_2824
AACDLWP59_22725 LWP59_22730
PDXPsed_2464
PSEAWY02_25075
PAUTPdca_48160(gcvT_2)
RXYRxyl_0192 Rxyl_0209 Rxyl_1565
TROtrd_0424 trd_0901 trd_A0792
STISthe_0208 Sthe_0782 Sthe_1118 Sthe_2713 Sthe_2797
OLEK0B96_04355
HDOMUK72_18085
HLMDV707_07675 DV707_09365 DV707_10535 DV707_15670 DV707_15690 DV707_16580 DV707_16605 DV707_16610 DV707_16820 DV707_16920 DV707_17025
HDFAArcSl_0610(ligM)
HLRHALLA_02090
 » show all
Reference
1  [PMID:9501423]
  Authors
Nishikawa S, Sonoki T, Kasahara T, Obi T, Kubota S, Kawai S, Morohoshi N, Katayama Y
  Title
Cloning and sequencing of the Sphingomonas (Pseudomonas) paucimobilis gene essential for the O demethylation of vanillate and syringate.
  Journal
Appl Environ Microbiol 64:836-42 (1998)
DOI:10.1128/AEM.64.3.836-842.1998
Reference
2  [PMID:15090517]
  Authors
Masai E, Sasaki M, Minakawa Y, Abe T, Sonoki T, Miyauchi K, Katayama Y, Fukuda M
  Title
A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate.
  Journal
J Bacteriol 186:2757-65 (2004)
DOI:10.1128/jb.186.9.2757-2765.2004
Reference
3  [PMID:15743951]
  Authors
Abe T, Masai E, Miyauchi K, Katayama Y, Fukuda M
  Title
A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6.
  Journal
J Bacteriol 187:2030-7 (2005)
DOI:10.1128/JB.187.6.2030-2037.2005
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.341
IUBMB Enzyme Nomenclature: 2.1.1.341
ExPASy - ENZYME nomenclature database: 2.1.1.341
BRENDA, the Enzyme Database: 2.1.1.341
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