KEGG   ENZYME: 2.1.1.90
Entry
EC 2.1.1.90                 Enzyme                                 
Name
methanol---corrinoid protein Co-methyltransferase;
methanol cobalamin methyltransferase;
methanol:5-hydroxybenzimidazolylcobamide methyltransferase;
MT 1 (ambiguous);
methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase;
mtaB (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methanol:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
methanol + a [Co(I) methanol-specific corrinoid protein] = a [methyl-Co(III) methanol-specific corrinoid protein] + H2O [RN:R04384]
Reaction(KEGG)
R04384;
(other) R09098
Substrate
methanol [CPD:C00132];
[Co(I) methanol-specific corrinoid protein]
Product
[methyl-Co(III) methanol-specific corrinoid protein];
H2O [CPD:C00001]
Comment
The enzyme, which catalyses the transfer of methyl groups from methanol to a methanol-specific corrinoid protein (MtaC), is involved in methanogenesis from methanol. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. Free cob(I)alamin can substitute for the corrinoid protein in vitro [2].
Inactivated by oxygen and other oxidizing agents, and reactivated by catalytic amounts of ATP and hydrogen.
History
EC 2.1.1.90 created 1989, modified 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K04480  methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase
Genes
DSXGD604_09795
DTIDesti_2034
CFMBJL90_02615
CCLClocl_0783
ANRAna3638_17590
DORDesor_2803
DMIDesmer_2205
DKUDesku_0051
FWADCMF_12970
TJRTherJR_0987
ELMELI_2003
EMTCPZ25_002645
ELIMB2M23_16850
AWOAwo_c22760(mttB18)
VPYHZI73_06080
MTAMoth_1209
MTHOMOTHE_c11860
MTHZMOTHA_c12720
TPZTph_c03590(mtaB)
MANAMAMMFC1_02497
STEDSPTER_30470
ALUSSTSP2_00335
MSTMsp_0184(mtaB3) Msp_0186(mtaB2) Msp_0188(mtaB1) Msp_0819(mtaB4)
METBAW729_03460 AW729_06185
MSIMsm_0515
MAROMarbSA_04050
MELMetbo_1023
MEWMSWAN_1082
MCUBMCBB_1154(mtaB)
MBAMbar_A0741 Mbar_A1064 Mbar_A3638
MBYMSBRM_0302 MSBRM_2353 MSBRM_3321
MBWMSBRW_0261 MSBRW_1094 MSBRW_3658
MBARMSBR2_2078 MSBR2_2319 MSBR2_3275
MBAKMSBR3_2096 MSBR3_2380 MSBR3_3273
MACMA_0455(mtaB1) MA_1616(mtaB3) MA_4392(mtaB2)
MMAMM_0175(mtaB) MM_1074(mtaB) MM_1647
MMAZMmTuc01_0182 MmTuc01_1725
MMJMSMAS_0227 MSMAS_1126 MSMAS_3023
MMACMSMAC_0233 MSMAC_1415 MSMAC_2486
MVCMSVAZ_0210 MSVAZ_0343 MSVAZ_1365
MEKMSKOL_0204 MSKOL_0326 MSKOL_1308
MLSMSLAZ_0239 MSLAZ_1380 MSLAZ_2955
METMMSMTP_0258 MSMTP_1140 MSMTP_2926
MEFMSWH1_0315 MSWH1_0524 MSWH1_1022
MEQMSWHS_0318 MSWHS_0538 MSWHS_1093
MSJMSSAC_0405 MSSAC_1598 MSSAC_4075
MSZMSSIH_0373 MSSIH_1187 MSSIH_3611
MSWMSSIT_0381 MSSIT_1254 MSSIT_3664
MTHRMSTHT_0083 MSTHT_1750 MSTHT_2137
MTHEMSTHC_0647 MSTHC_1146 MSTHC_1539
MHORMSHOH_0282 MSHOH_1348 MSHOH_3746
MFZAOB57_002020 AOB57_005220 AOB57_007940
MBUMbur_0812 Mbur_0814
MMETMCMEM_0944 MCMEM_0947 MCMEM_0949
MELOJ7W08_02955 J7W08_02965
MMHMmah_0976 Mmah_0978
MHAZBHR79_08685 BHR79_08695
MEVMetev_0944
MZHMzhil_1950 Mzhil_1952
MPYMpsy_0909 Mpsy_3036
MZIHWN40_04050 HWN40_07075
MHZMetho_0775 Metho_1601
RCIRCIX1974(mtaB)
MEAMMU439_02355
MAXMMALV_09470
MERMMINT_02820 MMINT_03160 MMINT_05920
MEARMpt1_c05090(mtaB)
MARCAR505_0949 AR505_0951
 » show all
Reference
1  [PMID:6438059]
  Authors
van der Meijden P, te Brommelstroet BW, Poirot CM, van der Drift C, Vogels GD.
  Title
Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri.
  Journal
J Bacteriol 160:629-35 (1984)
DOI:10.1128/JB.160.2.629-635.1984
  Sequence
Reference
2  [PMID:10215883]
  Authors
Sauer K, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin.
  Journal
Eur J Biochem 261:674-81 (1999)
DOI:10.1046/j.1432-1327.1999.00355.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.90
IUBMB Enzyme Nomenclature: 2.1.1.90
ExPASy - ENZYME nomenclature database: 2.1.1.90
BRENDA, the Enzyme Database: 2.1.1.90
CAS: 86611-98-5
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