KEGG   ENZYME: 2.1.3.10
Entry
EC 2.1.3.10                 Enzyme                                 
Name
malonyl-S-ACP:biotin-protein carboxyltransferase;
malonyl-S-acyl-carrier protein:biotin-protein carboxyltransferase;
MadC/MadD;
MadC,D;
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxyltransferase
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
Sysname
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxytransferase
Reaction(IUBMB)
a malonyl-[acyl-carrier protein] + a biotinyl-[protein] = an acetyl-[acyl-carrier protein] + a carboxybiotinyl-[protein] [RN:R08936]
Reaction(KEGG)
R08936
Substrate
malonyl-[acyl-carrier protein] [CPD:C01209];
biotinyl-[protein] [CPD:C18046]
Product
acetyl-[acyl-carrier protein] [CPD:C03939];
carboxybiotinyl-[protein] [CPD:C18047]
Comment
Derived from the components MadC and MadD of the anaerobic bacterium Malonomonas rubra, this enzyme is a component of EC 7.2.4.4, biotin-dependent malonate decarboxylase. The carboxy group is transferred from malonate to the prosthetic group of the biotin protein (MadF) with retention of configuration [2]. Similar to EC 4.1.1.87, malonyl-S-ACP decarboxylase, which forms part of the biotin-independent malonate decarboxylase (EC 4.1.1.88), this enzyme also follows on from EC 2.3.1.187, acetyl-S-ACP:malonate ACP transferase, and results in the regeneration of the acetyl-[acyl-carrier protein] [3].
History
EC 2.1.3.10 created 2008, modified 2018
Orthology
K20510  malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadC
K20511  malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadD
Genes
EFE: EFER_3130 EFER_3131
KPZ: KPNIH27_22440 KPNIH27_22445
KPB: FH42_14170 FH42_14175
KLW: DA718_03335(mdcE) DA718_03340
KGR: JJJ10_02970(mdcE) JJJ10_02975
CAMA: F384_01155 F384_01160
CTEL: GBC03_24695 GBC03_24700(mdcE)
SFO: Z042_24845 Z042_26175
DES: DSOUD_0385 DSOUD_0386
RPB: RPB_3295 RPB_3296
RPC: RPC_1059 RPC_1060
RCP: RCAP_rcp00127(mdcE) RCAP_rcp00128(mdcB)
ATI: AL072_31130 AL072_31135
AZZ: DEW08_20130(mdcE) DEW08_20135
RRU: Rru_A1324 Rru_A1325
RRF: F11_06850 F11_06855
PFT: JBW_01392 JBW_01393
 » show all
Reference
1  [PMID:9128730]
  Authors
Berg M, Hilbi H, Dimroth P
  Title
Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.
  Journal
Eur J Biochem 245:103-15 (1997)
DOI:10.1111/j.1432-1033.1997.00103.x
  Sequence
[ag:AAC45402 AAC45403]
Reference
2
  Authors
Micklefield, J., Harris, K.J., Groger, S., Mocek, U., Hilbi, H., Dimroth, P. and Floss, H.G.
  Title
Stereochemical course of malonate decarboxylase in Malonomonas rubra has biotin decarboxylation with retention.
  Journal
J Am Chem Soc 117:1153-1154 (1995)
Reference
3  [PMID:11902724]
  Authors
Dimroth P, Hilbi H
  Title
Enzymic and genetic basis for bacterial growth on malonate.
  Journal
Mol Microbiol 25:3-10 (1997)
DOI:10.1046/j.1365-2958.1997.4611824.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.10
IUBMB Enzyme Nomenclature: 2.1.3.10
ExPASy - ENZYME nomenclature database: 2.1.3.10
BRENDA, the Enzyme Database: 2.1.3.10
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