KEGG   ENZYME: 2.1.3.10
Entry
EC 2.1.3.10                 Enzyme                                 
Name
malonyl-S-ACP:biotin-protein carboxyltransferase;
malonyl-S-acyl-carrier protein:biotin-protein carboxyltransferase;
MadC/MadD;
MadC,D;
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxyltransferase
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
Sysname
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxytransferase
Reaction(IUBMB)
a malonyl-[acyl-carrier protein] + a biotinyl-[protein] = an acetyl-[acyl-carrier protein] + a carboxybiotinyl-[protein] [RN:R08936]
Reaction(KEGG)
R08936
Substrate
malonyl-[acyl-carrier protein] [CPD:C01209];
biotinyl-[protein] [CPD:C18046]
Product
acetyl-[acyl-carrier protein] [CPD:C03939];
carboxybiotinyl-[protein] [CPD:C18047]
Comment
Derived from the components MadC and MadD of the anaerobic bacterium Malonomonas rubra, this enzyme is a component of EC 7.2.4.4, biotin-dependent malonate decarboxylase. The carboxy group is transferred from malonate to the cofactor of the biotin protein (MadF) with retention of configuration [2]. Similar to EC 4.1.1.87, malonyl-S-ACP decarboxylase, which forms part of the biotin-independent malonate decarboxylase (EC 4.1.1.88), this enzyme also follows on from EC 2.3.1.187, acetyl-S-ACP:malonate ACP transferase, and results in the regeneration of the acetyl-[acyl-carrier protein] [3].
History
EC 2.1.3.10 created 2008, modified 2018
Orthology
K20510  malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadC
K20511  malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadD
Genes
EFE: EFER_3130 EFER_3131
KPZ: KPNIH27_22440 KPNIH27_22445
KPB: FH42_14170 FH42_14175
KLW: DA718_03335(mdcE) DA718_03340
KGR: JJJ10_02970(mdcE) JJJ10_02975
KPAS: LUW96_03545 LUW96_03550(mdcE)
KLC: K7H21_02980(mdcE) K7H21_02985
KLM: BWI76_25005 BWI76_25010
KLP: GUC22_02880(mdcE) GUC22_02885
CAMA: F384_01155 F384_01160
CTEL: GBC03_24695 GBC03_24700(mdcE)
SFO: Z042_24845 Z042_26175
RPB: RPB_3295 RPB_3296
RPC: RPC_1059 RPC_1060
RHOL: RBJ75_08045(mdcE) RBJ75_08050
RCP: RCAP_rcp00127(mdcE) RCAP_rcp00128(mdcB)
RAP: RHOA_4940(madC) RHOA_4941(madD)
ATI: AL072_31130 AL072_31135
AHU: A6A40_23020(mdcE) A6A40_23025
AZZ: DEW08_20130(mdcE) DEW08_20135
RRU: Rru_A1324 Rru_A1325
RRF: F11_06850 F11_06855
DES: DSOUD_0385 DSOUD_0386
PFT: JBW_01392 JBW_01393
 » show all
Reference
1  [PMID:9128730]
  Authors
Berg M, Hilbi H, Dimroth P
  Title
Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.
  Journal
Eur J Biochem 245:103-15 (1997)
DOI:10.1111/j.1432-1033.1997.00103.x
  Sequence
[ag:AAC45402 AAC45403]
Reference
2
  Authors
Micklefield J, Harris KJ, Groger S, Mocek U, Hilbi H, Dimroth P, Floss HG.
  Title
Stereochemical course of malonate decarboxylase in Malonomonas rubra has biotin decarboxylation with retention.
  Journal
J Am Chem Soc 117:1153-1154 (1995)
Reference
3  [PMID:11902724]
  Authors
Dimroth P, Hilbi H
  Title
Enzymic and genetic basis for bacterial growth on malonate.
  Journal
Mol Microbiol 25:3-10 (1997)
DOI:10.1046/j.1365-2958.1997.4611824.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.10
IUBMB Enzyme Nomenclature: 2.1.3.10
ExPASy - ENZYME nomenclature database: 2.1.3.10
BRENDA, the Enzyme Database: 2.1.3.10
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