Entry Name isonitrile lipopeptide synthase;
Class Transferases;BRITE hierarchy
Sysname (3R)-3-isocyano-fatty acyl-[acyl-carrier protein]:L-lysyl-[L-lysyl-carrier protein] (3R)-3-isocyano-fatty acyltransferase (hydrolysing)
Reaction(IUBMB) 2 (3R)-3-isocyanobutanoyl-[acyl-carrier protein] + L-lysyl-[L-lysyl-carrier protein] + 2 NADPH = (3R)-N-[(2S)-1-hydroxy-6-[(3R)-3-isocyanobutanamido]hexan-2-yl]-3-isocyanobutanamide + [L-lysyl-carrier protein] + 2 [acyl-carrier protein] + 2 NADP+ [RN:
R13508 ]
Reaction(KEGG) Substrate (3R)-3-isocyanobutanoyl-[acyl-carrier protein] [CPD:
C23044 ];
L-lysyl-[L-lysyl-carrier protein] [CPD:
C23045 ];
NADPH [CPD:
C00005 ]
Product (3R)-N-[(2S)-1-hydroxy-6-[(3R)-3-isocyanobutanamido]hexan-2-yl]-3-isocyanobutanamide [CPD:
C23046 ];
[L-lysyl-carrier protein] [CPD:
C23047 ];
[acyl-carrier protein] [CPD:
C00229 ];
NADP+ [CPD:
C00006 ]
Comment The enzyme, found in some actinobacterial species, is a non-ribosomal peptide synthase (NRPS). Adenylation and thiolation domains of the enzyme activate a lysine residue and load it on the enzyme (this activity is described separately as EC
6.2.1.77 , L-lysine---[L-lysyl-carrier protein] ligase). A condensation domain catalyses the condensation of two isonitrile-containing moieties to both amino groups of the lysine, a rare activity in non-ribosomal peptide biosynthesis. A reductase domain catalyses a four-electron reduction, releasing the product from the NRPS with a terminal alcohol group.
History EC 2.3.1.329 created 2025
Orthology K28320 isonitrile lipopeptide synthase
K28321 isonitrile lipopeptide synthase / L-lysine---[L-lysyl-carrier protein] ligase
Genes MBAI : MB901379_00190(ppsC_1)
MSAL : DSM43276_00574(dhbF_2)
ยป show all Taxonomy Reference Authors Chhabra A, Haque AS, Pal RK, Goyal A, Rai R, Joshi S, Panjikar S, Pasha S, Sankaranarayanan R, Gokhale RS.
Title Nonprocessive [2 + 2]e- off-loading reductase domains from mycobacterial nonribosomal peptide synthetases.
Journal Sequence Reference Authors Harris NC, Sato M, Herman NA, Twigg F, Cai W, Liu J, Zhu X, Downey J, Khalaf R, Martin J, Koshino H, Zhang W.
Title Biosynthesis of isonitrile lipopeptides by conserved nonribosomal peptide synthetase gene clusters in Actinobacteria.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 2.3.1.329
LinkDB All DBs 