KEGG   ENZYME: 2.3.2.16
Entry
EC 2.3.2.16                 Enzyme                                 
Name
lipid II:glycine glycyltransferase;
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:N6-glycine transferase;
femX (gene name);
alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine:glycine N6-glycyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine N6-glycyltransferase
Reaction(IUBMB)
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNAGly [RN:R08776]
Reaction(KEGG)
R08776
Substrate
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]
Product
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]
Comment
The enzyme from Staphylococcus aureus catalyses the transfer of glycine from a charged tRNA to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc (lipid II), attaching it to the N6 of the L-Lys at position 3 of the pentapeptide. This is the first step in the synthesis of the pentaglycine interpeptide bridge that is used in S. aureus for the crosslinking of different glycan strands to each other. Four additional Gly residues are subsequently attached by EC 2.3.2.17 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase) and EC 2.3.2.18 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase).
History
EC 2.3.2.16 created 2010
Pathway
ec00550  Peptidoglycan biosynthesis
ec01100  Metabolic pathways
Orthology
K11693  peptidoglycan pentaglycine glycine transferase (the first glycine)
Genes
DAKDaAHT2_1769
DALKDSCA_12040
SDOSD1155_00950
RAPRHOA_PB0096
SAUSA2057(fmhB)
SAVSAV2262(fmhB)
SAWSAHV_2246(fmhB)
SAHSaurJH1_2329
SAJSaurJH9_2288
SAMMW2180(fmhB)
SASSAS2152
SARSAR2346(fmhB)
SACSACOL2253(femX)
SAXUSA300HOU_2244(fmhB)
SAASAUSA300_2214
SAOSAOUHSC_02527
SAENWMN_2163(fmhB)
SADSAAV_2326(femX)
SUUM013TW_2220(fmhB)
SUVSAVC_10175
SUESAOV_2300c
SUJSAA6159_02168(fmhB)
SUKSAA6008_02299(fmhB)
SUCECTR2_2124
SUTSAT0131_02437
SUQHMPREF0772_10937(femX)
SUZMS7_2282(femX)
SUDST398NM01_2314
SUXSAEMRSA15_21600(fmhB)
SUWSATW20_23950(fmhB)
SUGSAPIG2314
SUFSARLGA251_20470(fmhB)
SAUASAAG_00090
SAUERSAU_002097(femX)
SAUNSAKOR_02225
SAUSSA40_2008(fmhB)
SAUUSA957_2092(fmhB)
SAUGSA268_2163(fmhB)
SAUZSAZ172_2361(fmhB)
SAUTSAI1T1_2016700
SAUJSAI2T2_1016710
SAUKSAI3T3_1016700
SAUQSAI4T8_1016710
SAUVSAI7S6_1016700(femX)
SAUWSAI5S5_1016640(femX)
SAUXSAI6T6_1016650(femX)
SAUYSAI8T7_1016680(femX)
SAUFX998_2246(fmhB)
SABSAB2134c(fmhB)
SUYSA2981_2200(fmhB)
SAUBC248_2299(fmhB)
SAUMBN843_23000
SAUCCA347_2342(femX)
SAURSABB_01408(femX)
SAUIAZ30_11940
SAUDCH52_07650
SAMSNI36_11150
SUHSAMSHR1132_20870
SERSERP1844(femX)
SEPSE_1835
SEPPSEB_01846
SEPSDP17_786(femX)
SHASH0790(fmtB)
SHHShL2_00689(fmhB)
SSPSSP0651
SCASCA_1750(fmhB)
SLGSLGD_00762
SLNSLUG_07600(fmhB)
SSDSPSINT_1923
SDTSPSE_0559(femX)
SDPNCTC12225_00638(femX)
SWAA284_03255
SPASSTP1_0738(femX)
SXYBE24_08730
SXLSXYLSMQ121_0683
SXOSXYL_00693(femX)
SHUSHYC_03095
SCAPAYP1020_1437(femX)
SSCHLH95_02915
SSCZRN70_03190
SAGQEP23_06995
SEQOSE1039_19250
SSIFAL483_01080
SCVA4G25_11375
SPETCEP67_05210
SLZB5P37_03375
SCOHBZ166_07685
SNLBJD96_03580
SKLC7J89_04775
SFQC7J90_05815
SHOMEGX58_07435
SMUSC7J88_05520
SCARDWB96_03440
SCHRDWB92_02920
SARLSAP2_07240(femX)
SPICSAMEA4384060_0782(fmhB)
SSHNCTC13712_02209(femX)
SSIMSAMEA4384339_2091(fmhB)
SDBCNQ82_10745
SLLOISP08_03435
SAULI6G39_02840
SSACI6I31_01240
SRAILN051_02565
SPSDJMB28_03915
SSCUCEP64_02190
SFFFOB90_04830
SSTESAMEA4384403_0513(femX)
MLENH3V22_06120
MVTI6J10_08530
MCLMCCL_0176
MCAKMCCS_02970(femX)
MACRBHM04_09350
MBOEHT586_01555
MARYLAU42_00975
MBRUMGG13_01025
SHVAAT16_01225
SBACBVH56_00520
JEAJEM45_06455
NAMPKPF49_00425
ESIExig_1829
EATEAT1b_2964
EANEab7_1680
EXMU719_10375
EXUESP131_12475
EAPKB235_10605
EACEKKI46_10305
GOTAXE85_03825
GEQCG018_05955
PLVERIC2_c17540(femX)
FRUM3M36_04945
AURHMPREF9243_0081(femX)
AUNAWM73_08530
ACGAWM71_03060
GADK8O88_06525
CPECPE2090
CPFCPF_2347
CPRCPR_2059
CMAHC1I91_27125
ASMMOUSESFB_0039
ASOSFBmNL_00054
ASBRATSFB_0040
CPRFK7H06_11510
SARJHH195_08435
CLOHMPREF0868_0028(femX)
CTHDCDO33_14085
CCELCCDG5_0870
RALRumal_2891
RUSRBI_II00393
SMANC12CBH8_21090
ACOLK5I23_07245
SWOSwol_2417
SLPSlip_2315
SALQSYNTR_2105
CALKHUE98_04320
DSYDSY4943
DHDDhaf_4843
DDHDesde_4155
DDLDesdi_3466
DMTDESME_15595
SGYSgly_3298
DORDesor_5547
DAIDesaci_4652
DMIDesmer_4584
DEDDHBDCA_p2902
DECDCF50_p2904
DRSDEHRE_14345
TFRBR63_12640
FWADCMF_15565
AACXDEACI_0382
HMOHM1_1069
HCVFTV88_0926
VGUHYG85_21890 HYG85_23265
VPYHZI73_13370
ACAEHYG86_15825 HYG86_15830
TMRTmar_0525
THEFE1B22_06245
THEPDYI95_002370
FMSM1R53_05170
MTAMoth_2408
MTHOMOTHE_c24880(femX)
MTHZMOTHA_c25600(femX)
TPZTph_c27860(femX)
MASMahau_2220
TEPTepRe1_2456
TAETepiRe1_2642
TOCToce_0288
KMEH0A61_01136(femX)
FMAFMG_0801
BULLJOS54_03220
TURAT726_00450
TSGHLK68_13280
CCYCSCMU_12590
AARCG127AT_14575
LEULeucomu_05190
LVIG7068_03825
LLUTK1X41_04000 K1X41_04010
AREAL755_05785
AAQAOC05_02560
ARWMB46_02210
ARTHC3B78_05805
AAGINCTC2676_1_00769(femX_2)
AJGKKR91_02795 KKR91_04785
ASUNKG104_02505 KG104_04860
ASUFMNQ99_04835
PNVJMY29_04495
ACHAchl_1256
APNAsphe3_12130
PSNINIBR502771_19550
RDNHMPREF0733_10577
RAMAIDM48_02020
SATKSA2016_1436
YIMJ5M86_06110
ALXLVQ62_14070
SANWG7063_08390
PEIH9L10_00725
PBROHOP40_15170
TPECHLG82_08500
WIKH8R18_04045
BLEMBL8807_00145
RMARGBA65_14375
PCATPcatena_04000
KOVK9N68_26830
TOQHCG51_12960
CAGCagg_3053
TROtrd_0929
ATMANT_22610
ABATCFX1CAM_0314
ABAOBEQ56_03870
CAPCLDAP_31480
PBFCFX0092_A1065
THUGKNN16_00430
PMETG4Y79_19800 G4Y79_19805
TPEDTPE_2360
ASXCDL62_03775
ALKQM9189_10600
SAALL336_0559
SRBP148_SR1C001G0766
SRGXF24_00420
CAQAMICH65_0261
TMGUS01_C0001G0727(femAB)
BGWVE98_C0001G0551(femX)
 » show all
Reference
1  [PMID:15228543]
  Authors
Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I
  Title
In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
  Journal
Mol Microbiol 53:675-85 (2004)
DOI:10.1111/j.1365-2958.2004.04149.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.16
IUBMB Enzyme Nomenclature: 2.3.2.16
ExPASy - ENZYME nomenclature database: 2.3.2.16
BRENDA, the Enzyme Database: 2.3.2.16
LinkDB

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