KEGG ENZYME: 2.4.1.125

ENZYME: 2.4.1.125

Entry

EC 2.4.1.125                Enzyme

Name

sucrose---1,6-alpha-glucan 3(6)-alpha-glucosyltransferase;
water-soluble-glucan synthase (misleading);
GTF-I;
GTF-S;
GTF-SI;
sucrose-1,6-alpha-glucan 3(6)-alpha-glucosyltransferase;
sucrose:1,6-alpha-D-glucan 3-alpha- and 6-alpha-glucosyltransferase;
sucrose:1,6-, 1,3-alpha-D-glucan 3-alpha- and 6-alpha-D-glucosyltransferase;
sucrose:1,6-alpha-D-glucan 3(6)-alpha-D-glucosyltransferase;
gtfB (gene name);
gtfC (gene name);
gtfD (gene name)

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

sucrose:(1->6)-alpha-D-glucan 3(6)-alpha-D-glucosyltransferase

Reaction(IUBMB)

(1) sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1 [RN:R02120 R06066];
(2) sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + (1->3)-alpha-D-glucosyl-[(1->6)-alpha-D-glucosyl]n

Reaction(KEGG)

R02120 R06066(G)

Substrate

sucrose [CPD:C00089];
[(1->6)-alpha-D-glucosyl]n

Product

D-fructose [CPD:C00095];
[(1->6)-alpha-D-glucosyl]n+1;
(1->3)-alpha-D-glucosyl-[(1->6)-alpha-D-glucosyl]n

Comment

The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. This enzyme extends (1->6)-alpha-D-glucans by both alpha(1->3) and alpha(1->6) linkages, with one of the linkage types being dominant. cf. EC 2.4.1.140, alternansucrase.

History

EC 2.4.1.125 created 1984

Reference

1 [PMID:6216919]

Authors

Mukasa H, Shimamura A, Tsumori H.

Title

Purification and characterization of basic glucosyltransferase from Streptococcus mutans serotype c.

Journal

Biochim Biophys Acta 719:81-9 (1982)
DOI:10.1016/0304-4165(82)90310-5

Reference

2 [PMID:6461359]

Authors

Shimamura A, Tsumori H, Mukasa H.

Title

Purification and properties of Streptococcus mutans extracellular glucosyltransferase.

Journal

Biochim Biophys Acta 702:72-80 (1982)
DOI:10.1016/0167-4838(82)90028-0

Reference

3 [PMID:2937877]

Authors

Tsumori H, Shimamura A, Mukasa H.

Title

Purification and properties of extracellular glucosyltransferase synthesizing 1,6-, 1,3-alpha-D-glucan from Streptococcus mutans serotype a.

Journal

J Gen Microbiol 131:3347-53 (1985)
DOI:10.1099/00221287-131-12-3347

Reference

4 [PMID:8737492]

Authors

Fujiwara T, Tamesada M, Bian Z, Kawabata S, Kimura S, Hamada S

Title

Deletion and reintroduction of glucosyltransferase genes of Streptococcus mutans and role of their gene products in sucrose dependent cellular adherence.

Journal

Microb Pathog 20:225-33 (1996)
DOI:10.1006/mpat.1996.0021

Reference

5 [PMID:10234842]

Authors

Monchois V, Willemot RM, Monsan P

Title

Glucansucrases: mechanism of action and structure-function relationships.

Journal

FEMS Microbiol Rev 23:131-51 (1999)
DOI:10.1111/j.1574-6976.1999.tb00394.x

Reference

6 [PMID:21354427]

Authors

Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S

Title

Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans.

Journal

J Mol Biol 408:177-86 (2011)
DOI:10.1016/j.jmb.2011.02.028

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.125

IUBMB Enzyme Nomenclature:

2.4.1.125

ExPASy - ENZYME nomenclature database:

2.4.1.125

BRENDA, the Enzyme Database:

2.4.1.125

CAS:	81725-87-3

LinkDB

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