KEGG ENZYME: 2.4.1.201

ENZYME: 2.4.1.201

Entry

EC 2.4.1.201                Enzyme

Name

alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase;
MGAT4C (gene name);
N-acetylglucosaminyltransferase VI;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase VI;
uridine diphosphoacetylglucosamine-glycopeptide beta-1->4-acetylglucosaminyltransferase VI;
mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase;
GnTVI;
GlcNAc-T VI;
UDP-N-acetyl-D-glucosamine:2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->2)]-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:R05992]

Reaction(KEGG)

R05992(G);
(other) R04642

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Product

UDP [CPD:C00015];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Comment

Requires a high concentration of Mn2+ for maximal activity. The enzyme, characterized from hen oviduct membranes, participates in the processing of N-glycans in the Golgi apparatus. It transfers GlcNAc in beta1-4 linkage to a D-mannose residue that already has GlcNAc residues attached at positions 2 and 6 by beta linkages. No homologous enzyme appears to exist in mammals.

History

EC 2.4.1.201 created 1992, modified 2001, modified 2018

Pathway

ec00510

N-Glycan biosynthesis

ec01100

Metabolic pathways

Orthology

K13748

alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase C

Genes

HSA:	25834(MGAT4C)

PTR:	467085(MGAT4C)

PPS:	100992134(MGAT4C)

GGO:	101151757(MGAT4C)

PON:	100455323(MGAT4C)

NLE:	100584226(MGAT4C)

MCC:	700450(MGAT4C) 705617

MCF:	101866242(MGAT4C) 102131925

CSAB:

103230293 103238817(MGAT4C)

CATY:

105581112(MGAT4C)

PANU:

101006452(MGAT4C) 103878644

TGE:	112634753(MGAT4C)

RRO:	104655963(MGAT4C)

RBB:	108535046(MGAT4C) 108545046

TFN:	117074698 117089664(MGAT4C)

PTEH:

111522678 111537602(MGAT4C)

CJC:	100413583(MGAT4C)

SBQ:	101043109(MGAT4C)

CSYR:

103257393(MGAT4C)

MMUR:

105860669(MGAT4C)

OGA:	100951828(MGAT4C)

MMU:	67569(Mgat4c)

MCAL:

110303902(Mgat4c)

MPAH:

110326810(Mgat4c)

RNO:	299756(Mgat4c)

MCOC:

116075661(Mgat4c)

MUN:	110566104(Mgat4c)

CGE:	100760589(Mgat4c)

PLEU:

114702912(Mgat4c)

NGI:	103726602(Mgat4c)

HGL:	101708499 101725010(Mgat4c)

CPOC:

100726416(Mgat4c)

CCAN:

109700961(Mgat4c)

DORD:

105986819(Mgat4c)

DSP:	122119207(Mgat4c)

NCAR:

124983496

OCU:	100339976(MGAT4C) 100340174

OPI:	101532607(MGAT4C)

TUP:	102467784(MGAT4C)

CFA:	100682872 475418(MGAT4C)

VVP:	112914890(MGAT4C) 112934545

VLG:	121481334(MGAT4C)

AML:	100465193(MGAT4C)

UMR:	103660061(MGAT4C)

UAH:	113256203(MGAT4C)

UAR:	123780946(MGAT4C)

ELK:	111142256 111150946

LLV:

125107947

MPUF:

101692691(MGAT4C)

ORO:	101369336(MGAT4C)

EJU:	114211065(MGAT4C)

ZCA:	113923149(MGAT4C)

MLX:	117998923(MGAT4C)

FCA:	109501701(MGAT4C)

PYU:	121040665(MGAT4C)

PBG:	122472359(MGAT4C)

PTG:	102971513(MGAT4C)

PPAD:

109258125(MGAT4C)

AJU:	106976669(MGAT4C)

HHV:	120243391(MGAT4C)

BTA:	539661(MGAT4C)

BOM:	102269061(MGAT4C)

BIU:	109558955(MGAT4C)

BBUB:

102410250(MGAT4C)

CHX:	102169257(MGAT4C) 102172500

OAS:	101108733(MGAT4C)

ODA:	120855467 120858418(MGAT4C)

CCAD:

122427462(MGAT4C)

SSC:	414851(MGAT4C)

CFR:	102503714(MGAT4C)

CBAI:

105072000(MGAT4C)

CDK:	105084877(MGAT4C)

VPC:	102544620(MGAT4C)

BACU:

103004161(MGAT4C)

LVE:	103080459(MGAT4C)

OOR:	101281932(MGAT4C)

DLE:	111163584(MGAT4C)

PCAD:

102984869(MGAT4C)

PSIU:

116760966(MGAT4C)

ECB:	100051064(MGAT4C) 100065011

EPZ:	103555117 103558210(MGAT4C)

EAI:	106838333(MGAT4C) 106840148

MYB:	102240623 102253362(MGAT4C)

MYD:	102757109 102769462(MGAT4C)

MMYO:

118651197(MGAT4C)

MLF:	102439378(MGAT4C)

MNA:	107526839(MGAT4C) 107536007

PKL:	118729987(MGAT4C)

HAI:	109387992(MGAT4C)

DRO:	112304367(MGAT4C) 112318900

SHON:

118974878(MGAT4C) 119003929

AJM:	119059021(MGAT4C) 119060798

PDIC:

114514085(MGAT4C)

PHAS:

123813526(MGAT4C)

MMF:	118624483(MGAT4C)

RFQ:	117028036(MGAT4C)

PALE:

102882602(MGAT4C)

PGIG:

120607266(MGAT4C)

PVP:	105293222(MGAT4C)

RAY:	107508696(MGAT4C)

MJV:	108404809(MGAT4C)

TOD:	119248274(MGAT4C)

SARA:

101538424(MGAT4C)

LAV:	100654846(MGAT4C) 104845928

TMU:	101341397 101345845

DNM:	101433730(MGAT4C)

MDO:	100015496 100020942(MGAT4C) 103102801

GAS:	123247432 123250138(MGAT4C)

SHR:	100932381(MGAT4C) 105750316

PCW:	110213170(MGAT4C) 110219622

OAA:	100081053(MGAT4C) 100091422

GGA:	395415(MGAT4C) 427873(MGAT4C)

PCOC:

116226659(MGAT4C) 116238699

MGP:	100541955(MGAT4C) 100550363

CJO:	107311189(MGAT4C) 107324722

NMEL:

110388150 110397617(MGAT4C)

APLA:

101791726(MGAT4C) 101799353

ACYG:

106041520(MGAT4C) 106045877

AFUL:

116498846 116502268(MGAT4C)

TGU:	100228531(MGAT4C)

LSR:	110469164 110469371(MGAT4C)

SCAN:

103813418(MGAT4C) 103822368

PMOA:

120497272 120499839(MGAT4C)

OTC:	121338661(MGAT4C) 121345499

PRUF:

121357264(MGAT4C) 121365391

GFR:	102034112(MGAT4C) 102043367

FAB:	101809036(MGAT4C) 101815032

PHI:	102103698 102110314(MGAT4C)

PMAJ:

107205204(MGAT4C) 107214888

CCAE:

111933425(MGAT4C) 111939621

CCW:	104692465 104698425(MGAT4C)

ETL:	114064493(MGAT4C) 114068544

ZAB:	102060851(MGAT4C) 102065237

FPG:	101914300(MGAT4C) 101916943

FCH:	102045721(MGAT4C) 102049618

CLV:	102088784(MGAT4C) 102089679

EGZ:	104121996 104132945(MGAT4C)

NNI:	104011085 104022863(MGAT4C)

ACUN:

113488971 113491133(MGAT4C)

TALA:

104358634(MGAT4C) 104368412

PADL:

103916497 103918099(MGAT4C)

ACHC:

115334914 115336341(MGAT4C)

AAM:	106485644 106488510(MGAT4C)

AROW:

112965031(MGAT4C) 112965061

NPD:	112956234(MGAT4C) 112959176

DNE:	112981455(MGAT4C) 112983858

ASN:	102374657(MGAT4C) 102376706 102378177

AMJ:	102560247 102563452 102575494(MGAT4C)

CPOO:

109315437(MGAT4C) 109317586 109320931

GGN:	109295775(MGAT4C) 109299068

PSS:	102446260(MGAT4C) 102460254 102462087

CMY:	102934016 102938060 102941733(MGAT4C)

CPIC:

101936161 101937266 101947319(MGAT4C)

TST:	117869141(MGAT4C) 117876834

CABI:

116831643 116837314 116838998(MGAT4C)

MRV:	120395885(MGAT4C) 120404645 120405061

ACS:	100556708 100561638(mgat4c) 100562892

PVT:	110070064(MGAT4C) 110073381 110080923

SUND:

121919615 121927656 121930305(MGAT4C)

PBI:	103052083(MGAT4C) 103056592 103067082

PMUR:

107289197(MGAT4C) 107289270 107291092

TSR:	106543329 106545375 106548707(MGAT4C)

PGUT:

117655823(MGAT4C) 117663738 117665346

VKO:	123021414 123031944 123036175(MGAT4C)

PMUA:

114599446 114605745(MGAT4C)

ZVI:	118087646 118089492 118091132(MGAT4C)

GJA:	107107839 107111720 107123105(MGAT4C)

XLA:	108710250(mgat4c.L) 108713740(mgat4g.L) 108715280(mgat4g.S)

XTR:	100486336(mgat4c) 100489718

NPR:	108785666 108792416(MGAT4C)

RTEM:

120916984 120932064(MGAT4C)

BBUF:

120980409 120983210(MGAT4C)

BGAR:

122920881 122925844(MGAT4C)

DRE:	100333333(mgat4c) 492796(zgc:101663)

SRX:	107709235 107730506(mgat4c) 107740326 107746337

SANH:

107672194 107673483 107686247(mgat4c) 107697032

SGH:	107553950 107554219 107566074 107583810

CCAR:

109076300 109099101 109100200 109109693

CAUA:

113041006 113061513 113094049 113118293

PPRM:

120473933(zgc:101663) 120493289(mgat4c)

IPU:	108263961 108275769(zgc:101663) 124627963

PHYP:

113534472(mgat4c) 113545614

SMEO:

124395386(mgat4c) 124399071(zgc:101663)

TFD:	113650708(zgc:101663) 113652306(mgat4c)

AMEX:

103027198(mgat4c) 103036431

EEE:	113587801 113592123(mgat4c)

TRU:	101064767 101066579(mgat4c) 105418847

TNG:	GSTEN00012385G001 GSTEN00025653G001 GSTEN00025676G001

LCO:	104922610(mgat4c) 104925119 104933748

NCC:	104945386 104948068

CGOB:

115009692 115010151(mgat4c) 115010597

ELY:	117258290(zgc:101663) 117262063 117262816(mgat4c)

PLEP:

121946530(zgc:101663) 121950129 121950657(mgat4c)

SLUC:

116046844(mgat4c) 116046857 116058000

ECRA:

117947186(zgc:101663) 117949041(mgat4c)

PFLV:

114558668 114560033 114560790(mgat4c)

GAT:	120809353 120809461(mgat4c) 120828980

PPUG:

119196245(mgat4c) 119196346 119223297

MSAM:

119894451(mgat4c) 119905770

CUD:	121515449(mgat4c) 121515710 121516959

MZE:	101472188 101483483 106675125(mgat4c)

ONL:	100693195 100694611 100704471

OAU:	116314008 116314030 116322804(zgc:101663)

OLA:	101156719(mgat4c) 101173858 101175155

OML:	112152399 112152418 112159708(zgc:101663)

XMA:	102218707 102222649(mgat4c) 102226325

XCO:	114146072 114150652 114162050(mgat4c)

XHE:	116708141(mgat4c) 116724208 116737181

PRET:

103465731 103465750(mgat4c) 103466943

PFOR:

103147598(mgat4c) 103147605 103150232

PLAI:

106946939(mgat4c) 106952455 106955720

PMEI:

106920731 106930831(mgat4c) 106932588

GAF:	122831579 122835165(mgat4c) 122835691

CVG:	107093796 107099730(mgat4c) 107100989

CTUL:

119775468 119798056(mgat4c) 119799214

GMU:	124858877(mgat4c) 124876318 124879350

NFU:	107388342(mgat4c) 107391540 107395515

KMR:	108244220 108244231(mgat4c) 108249187(zgc:101663)

ALIM:

106523019 106526858(mgat4c) 106529854

NWH:	119415392 119415443(mgat4c) 119417207(zgc:101663)

AOCE:

111571046(mgat4c) 111576346 111582021

CSEM:

103380004 103384716 103398884(mgat4c)

POV:	109623979 109624028(mgat4c) 109630602

SSEN:

122775946 122776438(mgat4c) 122776933

HHIP:

117762789 117763482(mgat4c) 117765390

LCF:	108880860 108880875 108899620

SDU:	111222525(mgat4c) 111229827 111237016

SLAL:

111647753 111659931 111672170(mgat4c)

XGL:	120793480 120793483 120803652

HCQ:	109528599 109530880 109530900(mgat4c)

BPEC:

110155136(mgat4c) 110162118

MALB:

109956382 109956477(mgat4c) 109969969

SASA:

106561603 106573111 106573155

OTW:	112218868(mgat4c) 112219151 112221479 112252660 112253389

OMY:	110492713 110499683(mgat4c) 110499798 110524434 110524488

OGO:	123997662 124004004 124018217 124018671 124033449

ONE:	115108992 115109671 115115583 115122670 115133953

SALP:

111952907 111952978 111962732 111962745

SNH:	120053306 120053529 120061718 120065888 120065895

ELS:	105007116 105026418 105030750

SFM:	108918691 108928190 108931472

PKI:	111835193(mgat4c) 111855285

AANG:

118207630 118215556 118218736 118232199(mgat4c)

LOC:	102687023(mgat4c) 102690465

PSPA:

121299730 121300191 121318495(mgat4c) 121320295 121330096

ARUT:

117414479 117419564(mgat4c) 117428212 117965983

LCM:	102346343 102347820(MGAT4C)

CMK:	103175065 103179072 103185333(mgat4c)

RTP:	109910955(mgat4c) 109914242 109915501

BFO:	118413034 118413212 118413214

BBEL:

109469451 109469461 109469462 109474455 109474456 109474458 109474568

SPU:	105441386 580331

APLC:

110974061 110984449 110984711 110984722 110985805 110985807 110985808

SKO:

100372236

FCD:

110856945

PJA:

122265764

PCHN:

125044079

HAME:

121868829

PCLA:

123768461

PTRU:

123512426

HAZT:

108665683

CSCU:

111636137

SDM:

118180358

LGI:	LOTGIDRAFT_172688

PCAN:

112559553 112575653

GAE:	121382652 121383354

HRF:	124126230 124152739 124152785

HRJ:	124255965 124255977 124262739

CRG:	105320882 105324375 105324376 105325491 105330470 105338504 105338576 105340894 105342679 117680994 117683793 117683794 117683795 117683796

MYI:	110443458 110447857 110453151 110454675 110459894 110463378 110463896

PMAX:

117329199 117335820 117335983 117336373 117336392 117336407 117336523 117336524 117341467

MMER:

123535723 123536079 123536118 123536693 123536846 123537118

LAK:	106152265 106160495 106164659 106172365 106172639 106174249 106176399

NVE:	5503815 5513696 5517229 5517300 5521811

EPA:	110232308 110243200 110244311

ATEN:

116288335 116305189 116305197

ADF:	107328532 107333160 107333161 107336078 107344550 107347979 107348679 107352963

AMIL:

114947664 114949509 114959622 114969084 114969085 114969910 114971341

PDAM:

113678682 113681400 113681963 113682042 113682084 113686241

SPIS:

111319180 111325502 111325529 111328500 111328729

DGT:	114517430 114519996 114519998 114524837 114527166 114542530

XEN:	124441719 124441720 124442975 124443219 124443220 124443221 124446286

TAD:	TRIADDRAFT_32367 TRIADDRAFT_51821 TRIADDRAFT_55928 TRIADDRAFT_61341

APRO:

F751_5773

MBR:	MONBRDRAFT_30843

SRE:	PTSG_02232

SMIN:

v1.2.033022.t1(symbB.v1.2.033022.t1)

» show all

Reference

1 [PMID:2525556]

Authors

Brockhausen I, Hull E, Hindsgaul O, Schachter H, Shah RN, Michnick SW, Carver JP.

Title

Control of glycoprotein synthesis. Detection and characterization of a novel branching enzyme from hen oviduct, UDP-N-acetylglucosamine:GlcNAc beta 1-6 (GlcNAc beta 1-2)Man alpha-R (GlcNAc to Man) beta-4-N-acetylglucosaminyltransferase VI.

Journal

J Biol Chem 264:11211-21 (1989)

Reference

2 [PMID:10903319]

Authors

Taguchi T, Ogawa T, Inoue S, Inoue Y, Sakamoto Y, Korekane H, Taniguchi N

Title

Purification and characterization of UDP-GlcNAc: GlcNAcbeta 1-6(GlcNAcbeta 1-2)Manalpha 1-R [GlcNAc to Man]-beta 1, 4-N-acetylglucosaminyltransferase VI from hen oviduct.

Journal

J Biol Chem 275:32598-602 (2000)
DOI:10.1074/jbc.M004673200

Sequence

[gga:395415]

Reference

3 [PMID:10962001]

Authors

Sakamoto Y, Taguchi T, Honke K, Korekane H, Watanabe H, Tano Y, Dohmae N, Takio K, Horii A, Taniguchi N

Title

Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)- manalpha 1-R[GlcNAc to man]beta 1,4N-acetylglucosaminyltransferase VI.

Journal

J Biol Chem 275:36029-34 (2000)
DOI:10.1074/jbc.M005860200

Sequence

[gga:395415]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.201

IUBMB Enzyme Nomenclature:

2.4.1.201

ExPASy - ENZYME nomenclature database:

2.4.1.201

BRENDA, the Enzyme Database:

2.4.1.201

CAS:	119699-68-2

LinkDB

DBGET integrated database retrieval system