KEGG ENZYME: 2.4.1.219

ENZYME: 2.4.1.219

Entry

EC 2.4.1.219                Enzyme

Name

vomilenine glucosyltransferase;
UDPG:vomilenine 21-beta-D-glucosyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-glucose:vomilenine 21-O-beta-D-glucosyltransferase

Reaction(IUBMB)

UDP-glucose + vomilenine = UDP + raucaffricine [RN:R05882]

Reaction(KEGG)

R05882

Substrate

UDP-glucose [CPD:C00029];
vomilenine [CPD:C01761]

Product

UDP [CPD:C00015];
raucaffricine [CPD:C02074]

Comment

The indole alkaloid raucaffricine accumulates during the culture of Rauvolfia cell suspensions.

History

EC 2.4.1.219 created 2002

Pathway

ec00901

Indole alkaloid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K12695

raucaffricine beta-D-glucosidase / vomilenine glucosyltransferase

Genes

SMIL:

131002761

Reference

1 [PMID:10234858]

Authors

Warzecha H, Obitz P, Stockigt J.

Title

Purification, partial amino acid sequence and structure of the product of raucaffricine-O-beta-D-glucosidase from plant cell cultures of Rauwolfia serpentina.

Journal

Phytochemistry 50:1099-109 (1999)
DOI:10.1016/S0031-9422(98)00689-X

Reference

2 [PMID:10975500]

Authors

Warzecha H, Gerasimenko I, Kutchan TM, Stockigt J

Title

Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis.

Journal

Phytochemistry 54:657-66 (2000)
DOI:10.1016/S0031-9422(00)00175-8

Sequence

[ag:AAF03675]

Reference

Authors

Ruyter CM, Stockigt J.

Title

Enzymatic formation of raucaffricine, the major indole alkaloid of Rauwolfia serpentina cell-suspension cultures.

Journal

Helv Chim Acta 74:1707-1712 (1991)

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.219

IUBMB Enzyme Nomenclature:

2.4.1.219

ExPASy - ENZYME nomenclature database:

2.4.1.219

BRENDA, the Enzyme Database:

2.4.1.219

LinkDB

DBGET integrated database retrieval system