KEGG   ENZYME: 2.4.1.256
Entry
EC 2.4.1.256                Enzyme                                 
Name
dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase;
ALG10;
Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase;
dolichyl beta-D-glucosyl phosphate:D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol 2-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dolichyl beta-D-glucosyl-phosphate:alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol alpha-1,2-glucosyltransferase (configuration-retaining)
Reaction(IUBMB)
dolichyl beta-D-glucosyl phosphate + alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = dolichyl phosphate + alpha-D-Glc-(1->2)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol [RN:R06264]
Reaction(KEGG)
R06264(G)
Substrate
dolichyl beta-D-glucosyl phosphate [CPD:C01246];
alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
dolichyl phosphate [CPD:C00110];
alpha-D-Glc-(1->2)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Comment
This eukaryotic enzyme performs the final step in the synthesis of the lipid-linked oligosaccharide, attaching D-glucose in an alpha-1,2-linkage to the outermost D-glucose in the long branch. The lipid-linked oligosaccharide is involved in N-linked protein glycosylation of selected asparagine residues of nascent polypeptide chains in eukaryotic cells.
History
EC 2.4.1.256 created 2011, modified 2012
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K03850  alpha-1,2-glucosyltransferase
Genes
HSA144245(ALG10B) 84920(ALG10)
PTR451831 735940(ALG10)
PPS100971669 103784887
GGO101124291(ALG10B) 115930196
PON100459566(ALG10) 100462149
NLE100581890 100591198
MCC696470
MCF102129056
CSAB103218846
CATY105591351
PANU101022253
TGE112634711
RRO104680792(ALG10)
RBB108519010
TFN117090304(ALG10)
PTEH111540579
CJC100414177(ALG10)
SBQ101043301(ALG10)
CSYR103264238
MMUR105881001
LCAT123639860
OGA100965002(ALG10)
MMU380959(Alg10b)
MCAL110310698(Alg10)
MPAH110334834(Alg10)
RNO245960(Alg10)
MCOC116078139
MUN110553681
CGE100753866
MAUA101841249
PLEU114693038
MORG121461198
AAMP119823131(Alg10)
NGI103731291(Alg10)
HGL101710247(Alg10)
CPOC100727296
CCAN109698292
DORD105998696
DSP122106278(Alg10b)
NCAR124983720
OCU100338055(ALG10)
OPI101525145 101536621
TUP102499471(ALG10)
CFA477647
VVP112921049
VLG121479691
AML100480278
UMR103675386(ALG10)
UAH113257737
UAR123778865
ELK111161353
LLV125107652
MPUF101680548
ORO101366944(ALG10)
EJU114216559
ZCA113923094
MLX118025515
FCA101092445(ALG10)
PYU121038735
PBG122473124
PTG102958960(ALG10)
PPAD109268819(ALG10)
AJU106977425
HHV120226246
BTA539837(ALG10)
BOM102275661(ALG10)
BIU109559540
BBUB102406990(ALG10)
CHX102179173(ALG10)
OAS101104224
ODA120858550 120861172
CCAD122436496
SSC100525643(ALG10)
CFR102517383(ALG10)
CBAI105068267(ALG10)
CDK105090579(ALG10)
VPC102540783
BACU103014173(ALG10)
LVE103070115(ALG10)
OOR101285322(ALG10)
DLE111176903
PCAD102993649(ALG10)
PSIU116760799
ECB100064952
EPZ103561030
EAI106833444
MYB102241433(ALG10)
MYD102764376(ALG10)
MMYO118650960
MLF102442124(ALG10)
MNA107528375(ALG10)
PKL118729328
HAI109374405
DRO112318398
SHON118995213
AJM119045097
PDIC114513408
PHAS123812899
MMF118624357
RFQ117028609 117033946
PALE102879937
PGIG120618281
PVP105294101(ALG10)
RAY107498428
MJV108398423
TOD119247118
SARA101548674(ALG10)
TMU101348246
MDO100023650(ALG10)
GAS123249293
SHR100934370
PCW110199044(ALG10)
OAA100073466
GGA426734(ALG10)
PCOC116234298
MGP100550477
CJO107308112
NMEL110392405
AFUL116491895
LSR110467627
SCAN103813504
PMOA120510659
OTC121336372
PRUF121357383
GFR102034176(ALG10B)
FAB101806662(ALG10B)
PHI102106098(ALG10B)
PMAJ107204775(ALG10)
CCAE111923022(ALG10)
CCW104686912
ETL114060537
ZAB102073170
FCH102052568
CLV102097373
EGZ104123800(ALG10)
NNI104010788(ALG10)
PLET104614469
ACUN113491353
PADL103917275
ACHC115341947
AAM106487727(ALG10B)
AROW112974476
NPD112953316
DNE112980476
ASN102373115
AMJ102575178(ALG10B)
CPOO109315700
GGN109292115(ALG10B)
PSS102460425
CMY102934644
CPIC101950653
TST117875565
CABI116820936
MRV120391712
ACS100563081(alg10)
PVT110080795
SUND121931247
PBI103065196
TSR106550290
PGUT117679820
VKO123020061
PMUA114605086
ZVI118090609
GJA107113945
STOW125434893(ALG10)
XLA108711169(alg10.L) 432047(alg10.S)
XTR548946(alg10)
NPR108783835(ALG10)
RTEM120931798(ALG10)
BBUF120982280
BGAR122927895
DRE100331575(alg10)
SRX107721156 107726832
SANH107666210 107689747
SGH107569225 107600015
CCAR109069232 109072923
CAUA113057842 113098992
PPRM120477089(alg10)
MAMB125245521(alg10)
IPU108279920(alg10)
PHYP113532360
SMEO124401789(alg10)
TFD113645926(alg10)
AMEX103027857(alg10)
EEE113580192
TRU101071925(alg10)
TNGGSTEN00019842G001
LCO104938651
NCC104959434
CGOB115028281(alg10)
ELY117249306(alg10)
PLEP121961269(alg10)
SLUC116039778(alg10)
ECRA117938415(alg10)
PFLV114549935(alg10)
GAT120817839(alg10)
PPUG119210823(alg10)
MSAM119918097(alg10)
CUD121505231(alg10)
ALAT119032376(alg10)
MZE101470227
ONL100692975
OAU116334943(alg10)
OLA101157993(alg10)
OML112157106(alg10)
XMA102218101(alg10)
XCO114147003
XHE116736884
PRET103459531(alg10)
PFOR103155884(alg10)
PLAI106956515
PMEI106930280
GAF122826449(alg10)
CVG107099795
CTUL119776243(alg10)
GMU124882827(alg10)
NFU107383719
KMR108244056(alg10)
ALIM106524852
NWH119428006(alg10)
AOCE111582869
CSEM103382932(alg10)
POV109632917
SSEN122765817(alg10)
HHIP117757389(alg10)
HSP118101512(alg10)
LCF108899835(alg10)
SDU111224975
SLAL111667278
XGL120799478(alg10)
HCQ109520135(alg10)
BPEC110161661 110173956
MALB109960941(alg10)
BSPL114863080(alg10)
SASA106575988 106609113
OTW112214420 112216782(alg10)
OMY110490168(alg10) 110500280
OGO123994723(alg10) 124014144
ONE115106868 115133741
SALP111951196 111951453
SNH120031965(alg10) 120049317
ELS105028210(alg10)
SFM108920848
PKI111843423
AANG118232650(alg10)
LOC102683387(alg10)
PSPA121318335 121319760
ARUT117415264(alg10) 117419566
LCM102346649(ALG10B)
CMK103188282(alg10)
RTP109917198
BBEL109477306
CIN100184563
SCLV120339581
SPU590617
APLC110974016
DMEDmel_CG32076(Alg10)
DER6543808
DSE6605240
DSIDsimw501_GD14291(Dsim_GD14291)
DYADyak_GE21795
DAN6507873
DSR110190249
DPODpse_GA16660
DPE6598973
DMN108151774
DWI6645864
DGR6558396
DMODmoj_GI13687
DAZ108612615
DNV108652310
DHE111604477
DVI6623132
CCAT101463099
BOD106619133
MDE101892420
SCAC106090279
LCQ111686476
LSQ119602958 119610100
AGAAgaP_AGAP002420
ACOZ120948993
AARA120896014
ASTE118506924
AAG5569660
AALB109401118 115260171
CQUCpipJ_CPIJ009479
CPII120414421
CNS116342532
BCOO119074883 119084462
AME102655688
ACER107995976
ALAB122717913
BIM100744021
BBIF117214044
BVK117236308
BVAN117166803
BTER100648794
BPYO122572918
CCAL108624102
OBB114877717
MGEN117229717
NMEA116434760
CGIG122397558
SOC105204467
MPHA105837462
AEC105154554
ACEP105621143
PBAR105428180
VEM105563132
HST105188845
DQU106744200
CFO105248857
FEX115238700
LHU105668917
PGC109854307
OBO105277645
PCF106786294
PFUC122519537
VPS122630711
NVI103316515
CSOL105367866
TPRE106651400
MDL103568569
CGLO123260035
FAS105267612
DAM107046715
AGIF122858116
CCIN107269669
TCA661882
DPA109532867
SOY115889138
ATD109598081
CSET123321282
AGB108907485
NVL108567728
APLN108737554
PPYR116171116
OTU111425310
BMOR101745380
BMAN114246169
MSEX115450758
DPLKGM_215114
BANY112045717
PMAC106714301
PPOT106107541
PXU106115338
PRAP110993707
ZCE119839060
HAW110377221 110381419
TNL113505579
SLIU111351368
OFU114363192
PXY105383523 105387794
API100573721
AGS114125421
RMD113557253
DCI103507241
CLEC106666658
HHAL106684209
NLU111059688
PHUPhum_PHUM022240
FOC113209165
TPAL117651570
CSEC111872024
FCD110841871
DPXDAPPUDRAFT_127260
DMK116920788
PVM113822748
PJA122252983
PCHN125034108
HAME121869020
PCLA123771132
PTRU123501100
HAZT108672751
EAF111705132 111715534
LSM121127843
PPOI119089901 119090622
ISCIscW_ISCW008996
DSV119435042 119454043
RSAN119394453
RMP119173008 119185782
VDE111245947
VJA111271529
TUT107367937
DPTE113797750
DFR124499645
CSCU111632263
PTEP107437241
SDM118190831
CELCELE_T24D1.4(algn-10)
CBRCBG_03825(Cbr-tag-179)
BMYBM_BM5986(Bm5986)
LOALOAG_10306
NAINECAME_10107
HROHELRODRAFT_76765
LGILOTGIDRAFT_223840
PCAN112571593
BGT106052134
GAE121369480
HRF124136088
CRG105332981
MYI110461259
PMAX117330508
MMER123528693
OBI106876907
OSN115211841
LAK106172617
EGLEGR_06905
NVE5511131
EPA110243305
ATEN116296660
ADF107340959
AMIL114969054
PDAM113667013
SPIS111339426
DGT114521700
XEN124434258 124435361
HMG100197595
TADTRIADDRAFT_28403
AQU100637452
ATHAT5G02410(ALG10)
ALY9307041
CRB17883736
CSAT104708910 104734248 104768404
EUSEUTSA_v10013300mg
BRP103855464
BNA106429035 106433531 106433532 106448826 106448834
BOE106315969 106329468
RSZ108844297
THJ104809903
CPAP110825892
CIT102613758
CICCICLE_v10011519mg
PVY116110523 116121166
MINC123218268
TCC18597765
GRA105799851
GHI107889692 107891052
GAB108454810
DZI111295358
EGR104426472
GMX100792228 100792293
GSJ114396103 114418923
PVUPHAVU_008G100900g
VRA106754554
VAR108329529
VUN114183103
CCAJ109791189
APRC113863054
MTRMTR_7g060680
CAM101502941
LJALj0g3v0182329.1(Lj0g3v0182329.1) Lj0g3v0182329.2(Lj0g3v0182329.2)
ADU107474865
AIP107625566
AHF112727758 112747851
LANG109361297
FVE101293604
RCN112192458
PPER18772348
PMUM103339514
PAVI110761035
PDUL117631448
MDM103427573 103430439
ZJU107430592
MNT21404159
CSV101214003
CMO103490227
BHJ120069892
MCHA111013674
CMAX111466254
CMOS111438157
CPEP111782301
RCU8258446
JCU105649562
HBR110631521
MESC110605349
POP18099975
PEU105122465
PALZ118053372
JRE108999020
QSU111985375 111987350 112003757 112019671
QLO115969404 115995298
TWL119988813
VVI100249516
VRI117921241
SLY101252805
SPEN107029414
SOT102605691
CANN107842996
NTA107796503 107825705 107826089
NSY104223128
NTO104102446
NAU109237223
INI109159141
ITR116028526
SIND105174445
OEU111410869
EGT105952955
SSPL121795412 121798956
HAN110915476
ECAD122606228
LSV111887176
CCAV112522659
DCR108215959 108218658
CSIN114297073
BVG104889508
SOE110775635
CQI110710652
NNU104592573
MING122067477 122082087
TSS122645826
PSOM113309673 113344964
NCOL116254944 116268050
OSA4336325
DOSAOs04t0503700-01(Os04g0503700)
OBR102720597
BDI100827541
ATS109781116
TDC119355652 119364824
TAES123045956 123053822 123189691
TUA125538893
SBI110436308
ZMA100384825
SITA101753177
SVS117865864
PVIR120642432 120681852
PHAI112898931
PDA103708230
EGU105040462
MUS103991712
DCT110105454
PEQ110028102
AOF109846602
ATR18437339
SMOSELMODRAFT_96516(GT59A1)
PPP112280910
MNGMNEG_0009
CSLCOCSUDRAFT_28810
CVRCHLNCDRAFT_136022
APROF751_1473
CMECYME_CMS117C
GSLGasu_44200
CCPCHC_T00009283001
SCEYGR227W(DIE2)
SEUBDI49_2191
SPAOSPAR_G04380
AGOAGOS_ADL138C
ERCEcym_2078
KLAKLLA0_E15797g
KMXKLMA_60055(ALG10)
LTHKLTH0C01782g
VPOKpol_1050p59
ZROZYRO0B15202g
CGRCAGL0L06556g
NCSNCAS_0F00630(NCAS0F00630)
NDINDAI_0D02500(NDAI0D02500)
TPFTPHA_0A05070(TPHA0A05070)
TBLTBLA_0B07410(TBLA0B07410)
TDLTDEL_0G01240(TDEL0G01240)
TGBHG536_0A01240
KAFKAFR_0B04220(KAFR0B04220)
KNGKNAG_0A01600(KNAG0A01600)
ZMKHG535_0E01190
PPAPAS_chr1-4_0475
DHADEHA2B14520g
PICPICST_43096(DIE2)
PGUPGUG_02372
SPAASPAPADRAFT_56279(ALG10)
LELLELG_02835
CALCAALFM_C305340WA(DIE2)
CTPCTRG_02349
COTCORT_0C05060
CDUCD36_85320
CTENCANTEDRAFT_99436
YLIYALI0B17226g
CLUCLUG_00384
CLUSA9F13_08g01925
CAURCJI97_000373
PKZC5L36_0D05790
BBRXBRETT_002280
OPAHPODL_04758
SLUDSCDLUD_004496
NCRNCU16845
NTENEUTE1DRAFT128956(NEUTE1DRAFT_128956)
SMPSMAC_03022
PANPODANSg3599
TTTTHITE_2122404
MTMMYCTH_2069347
CTHRCTHT_0037550
MGRMGG_03990
PPEIPpBr36_04467
SSCKSPSK_08598
FGRFGSG_09668
FPUFPSE_04272
FVRFVEG_15829
FOXFOXG_08401
NHENECHADRAFT_32266
TRETRIREDRAFT_56812
TRRM419DRAFT_72561
MAWMAC_03989
MAJMAA_07026
CMTCCM_00015
PLJVFPFJ_06531
VALVDBG_01354
VDAVDAG_07893
CFJCFIO01_03790
CHIGCH63R_13556
SAPOSAPIO_CDS7584
ELAUCREL1_8882
PFYPFICI_04985
SSLSS1G_13971
BFUBCIN_15g04220(Bcdie2)
MBEMBM_06558
PSCOLY89DRAFT_596356
GLZGLAREA_03998
ANIAN5902.2
AFMAFUA_2G11080
ACTACLA_070040
NFINFIA_086370
AORAO090026000548
ANGANI_1_404024(An02g02980)
AFVAFLA_005731
ALUCAKAW2_21364S(ALG10)
ACHEACHE_40624A(ALG10)
APUUAPUU_10922S(ALG10)
PCSPc15g00710
PDPPDIP_10550
TMFPMAA_048310
TRGTRUGW13939_07770
CIMCIMG_12056(CIMG07985)
CPWCPC735_020280
PBLPAAG_06349
PBNPADG_01248
ABEARB_01400
TVETRV_02220
AJEHCAG_07825
BGHBDBG_07360
PNOSNOG_04972
PTEPTT_02199
BZECOCCADRAFT_99407
BSCCOCSADRAFT_175256
BORCOCMIDRAFT_32615
AALTCC77DRAFT_1052072
ZTRMYCGRDRAFT_50788
PFJMYCFIDRAFT_41825
FFUCLAFUR5_13593
BCOMBAUCODRAFT_88813
NPAUCRNP2_2480
TMLGSTUM_00010811001
SPOSPAC56F8.06c(alg10)
TVSTRAVEDRAFT_159711
DSQDICSQDRAFT_75042
PCOPHACADRAFT_84564
SHSSTEHIDRAFT_90298
HIRHETIRDRAFT_59746
PSQPUNSTDRAFT_94897
ADLAURDEDRAFT_78871
FMEFOMMEDRAFT_133672
GTRGLOTRDRAFT_69230
LBCLACBIDRAFT_399396(ALG10)
MRRMoror_12719
MOREE1B28_000830
CCICC1G_13631
SCMSCHCODRAFT_47266
ABPAGABI1DRAFT50659(AGABI1DRAFT_50659)
CPUTCONPUDRAFT_45145
SLASERLADRAFT_367092
UMAUMAG_05547
PFPPFL1_05107
MRTMRET_0177
MSYMMSY001_2039(DIE2)
PGRPGTG_13505
MLRMELLADRAFT_41465
SREPTSG_07662
DDIDDB_G0277817(alg10)
DPPDICPUDRAFT_85245
ACANACA1_006100
TPVTP01_1227
TGOTGME49_263690
TETTTHERM_00316440
PTMGSPATT00015067001
SMINv1.2.021974.t1(symbB.v1.2.021974.t1)
PIFPITG_03060 PITG_21941
PSOJPHYSODRAFT_477641
SPARSPRG_10004
GTTGUITHDRAFT_102443
NGRNAEGRDRAFT_79285
CMAIBFX80_04415
JEUBJP62_11900
DEEHQN60_05330
CHIZHQ393_13865
 » show all
Reference
1  [PMID:9597543]
  Authors
Burda P, Aebi M
  Title
The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation.
  Journal
Glycobiology 8:455-62 (1998)
DOI:10.1093/glycob/8.5.455
  Sequence
[sce:YGR227W]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.256
IUBMB Enzyme Nomenclature: 2.4.1.256
ExPASy - ENZYME nomenclature database: 2.4.1.256
BRENDA, the Enzyme Database: 2.4.1.256
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