linear (1->4)-alpha-D-glucan:(1->4)/(1->6)-alpha-D-glucan 6-alpha-D-glucosyltransferase
Reaction(IUBMB)
formation of a linear isomalto/malto-polysaccharide from linear malto-oligosaccharides
Comment
The enzyme, originally discovered in lactic acid bacteria but later found in other organisms, is similar to EC 2.4.1.395, reuteransucrase, yet is not able to act on sucrose. The enzyme, which belongs to the glycoside hydrolase 70 (GH70) family, possesses both hydrolase and transglycosylase activities, cleaving alpha(1->4) linkages from the non-reducing end of linear maltooligosaccharides and synthesizing linear alpha(1->6)-glucan chains. It also possesses an endo-alpha(1->4)-glycosidase activity. Due to its narrow binding groove, it is not able to act on branched substrates. cf. EC 2.4.1.396, 4,6-alpha-glucanotransferase (linear and branched substrates, branched products).
Kralj S, van Geel-Schutten GH, Dondorff MMG, Kirsanovs S, van der Maarel MJEC, Dijkhuizen L.
Title
Glucan synthesis in the genus Lactobacillus: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains.
Kralj S, Grijpstra P, van Leeuwen SS, Leemhuis H, Dobruchowska JM, van der Kaaij RM, Malik A, Oetari A, Kamerling JP, Dijkhuizen L.
Title
4,6-alpha-glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families 13 and 70.
Structural characterization of linear isomalto-/malto-oligomer products synthesized by the novel GTFB 4,6-alpha-glucanotransferase enzyme from Lactobacillus reuteri 121.
Te Poele EM, van der Hoek SE, Chatziioannou AC, Gerwig GJ, Duisterwinkel WJ, Oudhuis LAACM, Gangoiti J, Dijkhuizen L, Leemhuis H.
Title
GtfC Enzyme of Geobacillus sp. 12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-alpha-Glucanotransferase That Synthesizes a Linear Alternating (alpha1 --> 6)/(alpha1 --> 4) alpha-Glucan and Delays Bread Staling.
