The enzyme from the shark Heterodontus portusjacksoni is able to sulfate the C27 bile salts 5beta-scymnol (the natural bile salt) and 5alpha-cyprinol (the carp bile salt). Enzyme activity is activated by Mg2+ but inhibited by the product 5beta-scymnol sulfate.
Enzymic sulfation of bile salts. Partial purification and characterization of an enzyme from the liver of the shark Heterodontus portusjacksoni that catalyses the sulfation of the shark bile steroid 5 beta-scymnol.