KEGG ENZYME: 2.8.2.33

ENZYME: 2.8.2.33

Entry

EC 2.8.2.33                 Enzyme

Name

N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase;
GalNAc4S-6ST;
CHST15 (gene name);
3'-phosphoadenylyl-sulfate:[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine 6-O-sulfotransferase

Class

Transferases;
Transferring sulfur-containing groups;
Sulfotransferases

Sysname

3'-phosphoadenylyl-sulfate:[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine 6-O-sulfonotransferase

Reaction(IUBMB)

(1) 3'-phospho-5'-adenylyl sulfate + [dermatan]-4-O-sulfo-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine [RN:R10868];
(2) 3'-phospho-5'-adenylyl sulfate + [chondroitin]-4-O-sulfo-N-acetyl-D-galactosamine = adenosine 3',5'-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine [RN:R10869]

Reaction(KEGG)

R10868 R10869

Substrate

3'-phospho-5'-adenylyl sulfate [CPD:C00053];
[dermatan]-4-O-sulfo-N-acetyl-D-galactosamine;
[chondroitin]-4-O-sulfo-N-acetyl-D-galactosamine

Product

adenosine 3',5'-bisphosphate [CPD:C00054];
[dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine;
[chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine

Comment

The enzyme is activated by divalent cations and reduced glutathione. The enzyme from human transfers sulfate to position 6 of both internal residues and non-reducing terminal GalNAc 4-sulfate residues of chondroitin sulfate and dermatan sulfate. Oligosaccharides derived from chondroitin sulfate also serve as acceptors but chondroitin sulfate E, keratan sulfate and heparan sulfate do not. Differs from EC 2.8.2.17, chondroitin 6-sulfotransferase, in being able to use both chondroitin and dermatan as effective substrates

History

EC 2.8.2.33 created 2005, modified 2010

Pathway

ec00532

Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate

Orthology

K08106

N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase

Genes

HSA:	51363(CHST15)

PTR:	466249(CHST15)

PPS:	100988573(CHST15)

GGO:	101139112(CHST15)

PON:	100446124(CHST15)

PPYG:

129006179(CHST15)

NLE:	100593528(CHST15)

HMH:	116482375(CHST15)

SSYN:

129464857(CHST15)

MCC:	693845(CHST15)

MCF:	102140207(CHST15)

MTHB:

126962561

MNI:	105470073(CHST15)

CSAB:

103216659(CHST15)

CATY:

105578010(CHST15)

PANU:

101016703(CHST15)

TGE:	112631386(CHST15)

MLEU:

105544130(CHST15)

RRO:	104657648(CHST15)

RBB:	108519585(CHST15)

TFN:	117065294(CHST15)

PTEH:

111550715(CHST15)

CANG:

105503344(CHST15)

CJC:	100400214(CHST15)

SBQ:	101052289(CHST15)

CIMI:

108282101(CHST15)

ANAN:

105717965(CHST15)

CSYR:

103249734(CHST15)

MMUR:

105880351(CHST15)

LCAT:

123649822(CHST15)

PCOQ:

105808721(CHST15)

OGA:	100951575(CHST15)

MMU:	77590(Chst15)

MCAL:

110298310(Chst15)

MPAH:

110322292(Chst15)

RNO:	286974(Chst15)

MCOC:

116103105(Chst15)

ANU:	117702691(Chst15)

ASYL:

127694988(Chst15)

MUN:	110548350(Chst15)

CGE:	100768085(Chst15)

MAUA:

101835371(Chst15)

PROB:

127228453(Chst15)

PLEU:

114692647(Chst15)

MORG:

121457952(Chst15)

MFOT:

126498563

AAMP:

119826106(Chst15)

NGI:	103729255(Chst15)

HGL:	101726691(Chst15)

CPOC:

100713991(Chst15)

CCAN:

109691645(Chst15)

DORD:

105989753(Chst15)

DSP:	122126867(Chst15)

PLOP:

125345968(Chst15)

NCAR:

124985372

MMMA:

107152847(Chst15)

ITI:	101960546(Chst15)

OCU:

100343176

OPI:	101520239(CHST15)

TUP:	102470510(CHST15)

GVR:

103606991

CFA:	486928(CHST15)

CLUD:

112672462(CHST15)

VVP:	112928825(CHST15)

VLG:	121484521(CHST15)

NPO:	129493599(CHST15)

AML:	100472799(CHST15)

UMR:	103658000(CHST15)

UAH:	113252027(CHST15)

UAR:	123799992(CHST15)

ELK:

111145346

LLV:

125084758

MPUF:

101670516(CHST15)

MNP:	132016855(CHST15)

MLK:	131830578(CHST15)

NVS:	122901194(CHST15)

ORO:	101386186(CHST15)

EJU:	114221788(CHST15)

ZCA:	113922968(CHST15)

MLX:	118027443(CHST15)

NSU:	110592710(CHST15)

LWW:	102735859(CHST15)

FCA:	101098166(CHST15)

PYU:	121037346(CHST15)

PCOO:

112854690(CHST15)

PBG:	122493350(CHST15)

PVIV:

125147486(CHST15)

LRUF:

124507205

PTG:	102959008(CHST15)

PPAD:

109252307(CHST15)

PUC:

125933473

AJU:

106971732

HHV:	120235557(CHST15)

BTA:	534506(CHST15)

BOM:	102270502(CHST15)

BIU:	109553118(CHST15)

BBUB:

102401618(CHST15)

BBIS:

105002533(CHST15)

CHX:	102179262(CHST15)

OAS:	101113976(CHST15)

BTAX:

128067790(CHST15)

CSUM:

138070151(CHST15)

ODA:	120873936(CHST15)

CCAD:

122445840(CHST15)

MREE:

136160192(CHST15)

MBEZ:

129555269(CHST15)

SSC:	100152393(CHST15)

CFR:	102518619(CHST15)

CBAI:

105067361(CHST15)

CDK:	105100278(CHST15)

VPC:	102531223(CHST15)

BACU:

103000709(CHST15)

BMUS:

118882958(CHST15)

LVE:	103088154(CHST15)

OOR:	101287863(CHST15)

DLE:	111172221(CHST15)

PCAD:

102980366(CHST15)

PSIU:

116741989(CHST15)

NASI:

112403842(CHST15)

ECB:	100057594(CHST15)

EPZ:	103548446(CHST15)

EAI:	106847753(CHST15)

MYB:	102256050(CHST15)

MYD:	102768574(CHST15)

MMYO:

118669623(CHST15)

MDT:	132214468(CHST15)

PKL:	118719607(CHST15)

EFUS:

103299330(CHST15)

MNA:	107532673(CHST15)

DRO:	112300259(CHST15)

SHON:

118990538(CHST15)

AJM:	119059523(CHST15)

PDIC:

114497466(CHST15)

PHAS:

123828781(CHST15)

MMF:	118632336(CHST15)

PPAM:

129077240(CHST15)

HAI:	109396924(CHST15)

RFQ:	117036007(CHST15)

PALE:

102882217(CHST15)

PGIG:

120608834(CHST15)

PVP:	105295696(CHST15)

RAY:	107515914(CHST15)

MJV:	108407072(CHST15)

TOD:	119234800(CHST15)

SARA:

101553025(CHST15)

SFUM:

130021493(CHST15)

SETR:

125994909(CHST15)

LAV:	100659572(CHST15)

TMU:

101354169

ETF:	101642433(CHST15)

DNM:	101429956(CHST15)

MDO:	100025063(CHST15)

GAS:	123238209(CHST15)

SHR:	100913832(CHST15)

AFZ:

127551673

PCW:	110192729(CHST15)

TVP:	118828600(CHST15)

PBRV:

138169588(CHST15)

OAA:	100086938(CHST15)

GGA:	423952(CHST15)

PCOC:

116231547(CHST15)

MGP:	100542904(CHST15)

CJO:	107316272(CHST15)

TPAI:

128077535(CHST15)

LMUT:

125693303(CHST15)

NMEL:

110399495(CHST15)

APLA:

101792968(CHST15)

ACYG:

106038980(CHST15)

CATA:

118248700(CHST15)

AFUL:

116491465(CHST15)

TGU:	100221434(CHST15)

LSR:	110468429(CHST15)

SCAN:

103813773(CHST15)

PMOA:

120500013(CHST15)

OTC:	121341940(CHST15)

PRUF:

121350356(CHST15)

GFR:	102041118(CHST15)

FAB:	101819890(CHST15)

OMA:	130254866(CHST15)

PHI:	102110600(CHST15)

PMAJ:

107207093(CHST15)

CCAE:

111931391(CHST15)

CCW:	104694538(CHST15)

CBRC:

103621479(CHST15)

ACOE:

138112710(CHST15)

ETL:	114061005(CHST15)

ZAB:	102075205(CHST15)

ZLE:	135449858(CHST15)

ACHL:

103812243(CHST15)

SVG:	106855181(CHST15)

MMEA:

130582480(CHST15)

HRT:	120756170(CHST15)

SATI:

136363962(CHST15)

FPG:	101915195(CHST15)

FCH:	102046885(CHST15)

CCRI:

104156587(CHST15)

NNT:	104412285(CHST15)

SHAB:

115608842(CHST15)

ACUN:

113481638(CHST15)

TALA:

104360048(CHST15)

ACHC:

115348291(CHST15)

HALD:

104319860(CHST15)

HLE:	104840778(CHST15)

AGEN:

126043191

HHAR:

128147153(CHST15)

GCL:

127018044

CSTI:

104551196

LDI:	104349823(CHST15)

MNB:	103770195(CHST15)

DPUB:

104309134(CHST15)

PPUS:

135188648(CHST15)

AVIT:

104279428(CHST15)

BRHI:

104501097(CHST15)

EGZ:	104128314(CHST15)

NNI:	104022090(CHST15)

PCRI:

104029970(CHST15)

PCAO:

104048107(CHST15)

PADL:

103918531(CHST15)

AFOR:

103897782(CHST15)

FGA:	104077100(CHST15)

GSTE:

104252182(CHST15)

CLV:	102094121(CHST15)

MUI:

104539290

PGUU:

104465619(CHST15)

PLET:

104622666(CHST15)

EHS:	104511982(CHST15)

CMAC:

104483774(CHST15)

CUCA:

104058553(CHST15)

TEO:	104377049(CHST15)

BREG:

104638479(CHST15)

OHA:	104329472(CHST15)

ACAR:

104519062(CHST15)

CPEA:

104398672(CHST15)

CVF:	104293236(CHST15)

RTD:	128911514(CHST15)

AAM:	106494366(CHST15)

AROW:

112966021(CHST15)

NPD:	112947800(CHST15)

TGT:	104575021(CHST15)

DNE:	112992680(CHST15)

SCAM:

104141307(CHST15)

ASN:	102381919(CHST15)

AMJ:	102565249(CHST15)

CPOO:

109324158(CHST15)

GGN:	109287693(CHST15)

PSS:	102454589(CHST15)

CMY:	102936402(CHST15)

CCAY:

125640111(CHST15)

DCC:	119859084(CHST15)

CPIC:

101935858(CHST15)

TST:	117879803(CHST15)

CABI:

116834427(CHST15)

MRV:	120409583(CHST15)

ACS:	100557550(chst15)

ASAO:

132771037(CHST15)

PVT:	110070375(CHST15)

SUND:

121925611(CHST15)

PBI:	103065969(CHST15)

PMUR:

107293685(CHST15)

CTIG:

120306571(CHST15)

TSR:	106542215(CHST15)

PGUT:

117676705(CHST15)

APRI:

131200535(CHST15)

PTEX:

113439173(CHST15)

NSS:	113413087(CHST15)

VKO:	123031159(CHST15)

PMUA:

114597401(CHST15)

PRAF:

128414148(CHST15)

ZVI:	118096214(CHST15)

HCG:	128351751(CHST15)

GJA:	107123681(CHST15)

STOW:

125437833(CHST15)

EMC:	129332082(CHST15)

XLA:	108696098(chst15.L) 108697373

XTR:	100487411(chst15)

NPR:	108784782(CHST15)

RTEM:

120946712(CHST15)

BBUF:

121004060(CHST15)

BGAR:

122941653(CHST15)

DRE:	100537640 565523(chst15)

SRX:	107715409 107717116 107733550

SANH:

107663285 107674388(chst15)

SGH:	107553193 107569506(chst15) 107583521

CCAR:

109071133 109108050

CAUA:

113055905 113094577 113117208

CGIB:

127976452 128025594 128031654

PTET:

122333461 122362382(chst15)

LROH:

127173987 127180215(chst15)

OMC:	131522785(chst15) 131551471

PPRM:

120478115(chst15)

RKG:	130070648(chst15)

MAMB:

125255525 125255943 125268788(chst15)

CIDE:

127498666 127523208 127524183

CERY:

137006101(chst15) 137008059 137008288

MASI:

127410842 127423521 127426658 127455079

TROS:

130560817(chst15)

TDW:	130436434(chst15) 130439192

MANU:

129416928(chst15)

IPU:

108273603

IFU:	128616587(chst15)

PHYP:

113527444(chst15)

SMEO:

124388888(chst15)

TVC:

132861915

TRN:	134321152(chst15)

AMEX:

103044555(chst15) 111191342

CMAO:

118808973 118823949(chst15)

EEE:	113581575(chst15) 113591890

CHAR:

105900837(chst15)

TRU:	101062081 101075284(chst15)

TFS:	130528098 130534161

TNG:	GSTEN00024982G001 GSTEN00035920G001

LCO:	104925926(chst15) 104929338

NCC:	104942010(chst15) 104955507 104959243

TBEN:

117473958 117499809

CGOB:

115019915 115025046(chst15)

PGEO:

117459309 117465137

GACU:

117544719 117560954

EMAC:

134859235 134871411

ELY:	117247421 117247834

EFO:	125881109 125903080

PLEP:

121954714 121959321

SLUC:

116055643 116061429 116061431

ECRA:

117936794 117960717

ESP:	116670763(chst15) 116705484

PFLV:

114548070(chst15) 114548386 114572655

GAT:	120819556 120820791

PPUG:

119213271 119214626

AFB:	129091147 129092273

CLUM:

117744455 117749243

PSWI:

130204095 130207462

CANA:

137779913 137789660(chst15)

CVE:	137879696 137892051

MSAM:

119916242 119917226

SCHU:

122867805 122884663

CUD:	121511519 121527867

SPUL:

138588601 138593582

ALAT:

119009549 119034142

ASTR:

137588221 137603695

MZE:	101469675 101474113 112430020

ONL:	100691611(chst15) 100706438

OAU:	116322216 116328824

OLA:	101167818 101174629(chst15)

OML:	112154681 112161398

CSAI:

133418898 133463347

XMA:	102220050(chst15) 102227019

XCO:	114137376 114152214(chst15)

XHE:	116713849 116727402(chst15)

PRET:

103476751 103481828(chst15)

PFOR:

103133574 103150529(chst15)

PLAI:

106947801 106951739(chst15)

PMEI:

106903903 106922404(chst15)

GAF:	122823780 122842744

PPRL:

129359690 129368278

CVG:	107090219(chst15) 107091341

CTUL:

119776011 119783729

GMU:	124858744 124875775

NFU:	107375978 107387792(chst15)

KMR:	108243020 108246576

ALIM:

106512385 106513094 106516595(chst15)

NWH:	119419016 119423515 119423516

AOCE:

111570635 111580094

EJA:	138195263 138204847

BFRE:

138617664 138629405

MCEP:

125019352 125022611

CSEM:

103381963(chst15) 103386296 103386507 103386509 103386852 103390803

POV:	109626592(chst15) 109630365

SSEN:

122759072(chst15) 122767765

HHIP:

117753118 117775723

HSP:	118100984 118119053

PPLT:

128427348(chst15) 128442905

SMAU:

118283890 118287279

LCF:

108899055

SDU:	111220191 111235401

SLAL:

111660226(chst15) 111663760

XGL:	120794385 120796851

HCQ:	109510229 109513397(chst15)

SSCV:

125978929 125991435

SBIA:

133495565 133509898

PEE:	133409679 133417895

PTAO:

133469757 133485371

BPEC:

110165469(chst15)

MALB:

109962329 109962953(chst15)

BSPL:

114842100 114845571

SJO:	128372787 128381401

SSCO:

133991942 134003441

SASA:

106562767 106577042 106592094 106607484(chst15)

STRU:

115153249(chst15) 115190094 115193708

OTW:	112253074 112254813 121843130 121843300

OMY:	110502499 110513623 110518744 110529439

OGO:	124038274 124041223

ONE:	115113200 115135067

OKI:	109889975 109899113(chst15) 116359732

OKE:	118369755 118400013 127928789

SALP:

111952052 111977451(chst15)

SNH:	120017673 120045639

CCLU:

121553426 121583166 123489629

ELS:	105009432(chst15)

SFM:	108932796 108939475(chst15)

PKI:	111853711(chst15)

AANG:

118217845 118221821

CCOG:

133118378 133122693

LOC:	102695459(chst15)

PSPA:

121322441 121325255

ARUT:

117414709 117418037

PSEX:

120539466(chst15)

LCM:	102359407(CHST15)

CMK:	103185442(chst15)

RTP:

125485576

CPLA:

122561159

HOC:	132826270(chst15)

LERI:

129703985(chst15)

PMRN:

116940586(CHST15)

LRJ:	133343427(CHST15)

BFO:	118407732 118408527 118412818 118422193 118422340 118422953

BBEL:

109464225 109469316 109473883 109478427

SPU:	100888984 100890246 100890467 100892392 100892597 100892845 100893068 105437523 105443091 105446947 579940 582046 587805 590634 591575

LPIC:

129257033 129262065 129263903 129272163 129276451 129276646 129276924 129277831 129278972 129280916 129280918 129281267 129281485

APLC:

110977282 110980029 110986590

ARUN:

117288371 117289010 117299494

PVM:

113823241

PJA:

122261983

PCHN:

125041144

PMOO:

119591877

HAME:

121869961

PCLA:

123746156 138357369

CQD:

128685066

PTRU:

123505438

ESN:

127001612

MNZ:

135199948

MRJ:

136828127

PPOI:

119097992

LGI:	LOTGIDRAFT_109056 LOTGIDRAFT_114620 LOTGIDRAFT_156422 LOTGIDRAFT_214000 LOTGIDRAFT_221353

PVUL:

126811134 126815210 126816456 126816713 126817843 126824461

PCAN:

112553135 112553336 112558015 112558089 112558252 112559701 112559704 112559782 112561145 112561743 112563316 112563785 112564319 112564706 112564835 112571727 112571889 112572850

BGT:	106055664 106067553 106071469 106073090 106073097

GAE:	121376772 121379101 121383259 121388893 121389346 121389842

HRF:	124111692 124111819 124113087 124113995 124115126 124116095 124121918 124122990 124122998 124123834 124137248 124137415 124138040 124138558 124139046 124141513 124143399 124147920 124149095 124149650 124149678 124150292 124152807

HRJ:	124255203 124255512 124255546 124258009 124258011 124258035 124258036 124260937 124263188 124263708 124264092 124265369 124267300 124267302 124269560 124274899 124281295 124282621 124282822 124283319 124286446 124288781 124292325

HASI:

137258214 137258639 137259859 137260277 137260404 137260406 137260409 137265218 137265599 137272243 137272303 137274715 137276750 137278668 137278678 137278802 137283999 137284000 137285189 137286770 137286886 137287124 137287389 137287419 137287709 137287712 137287713 137287714 137287715 137287716 137287726 137287728 137287729 137288019 137294294 137298427

CRG:	105320560 105320595 105322577 105332349 105335131 105342523 136270210

CANU:

128155651 128159367 128159951 128165559 128168134 128173258 128173285 128181217

CVN:	111112404 111120059 111122371 111122916 111126462 111134518 111137757

OED:	125647882 125648016 125653322 125658007 125671361 125671689 125680313

MYI:	110441338 110446808 110449775 110450314 110451321 110452763 110453040 110455710 110456501 110462999 110464195 110464416 110464506 110464917

PMAX:

117316258 117322177 117325987 117331750 117337784 117339056 117339137 117341000 117341175

AIRR:

138309211 138310063 138310899 138319943 138320585 138322822 138323682 138325489 138333373 138334254 138334432 138334434

MMER:

123523605 123524957 123528855 123529416 123537421 123540782 123541018 123541034 123541384 123542375 123542505 123548273 123549849 123549944 123555499 123555930 123556091 123557160 123557835 123565757 123566046

RPHI:

132717794 132723378 132723379 132729154 132731070 132732235 132733120 132742654 132746000 132746103 132747951 132747967 132749239 132753491 132753565 132754311 132754590 132754638 132754652 132755659 132756621 132758682 132759681 132759682

DPOL:

127831184 127831791 127854184 127857710 127857711 127864817 127869798 127878396 127878397 127881668

MAEA:

128204214 128204400 128204415 128204416 128205134 128205686 128207005 128208538 128212312 128212313 128215387 128215398 128215984 128216455 128218691 128219247 128223179 128223180 128225674 128230974 128232310 128239154

OBI:

106868895

OSN:	115220419 115232487

LJP:	135467275 135467276 135468078 135468165 135468562 135475885

LAK:	106154999 106161352 106167071 106176800 106177814

LLON:

135483891 135484874 135488126 135488252 135494086 135496557 135497157 135499406 135499412 135500788 135502317

AQU:	105314102 105314958

CRE:	CHLRE_04g230732v5 CHLRE_09g387250v5 CHLRE_09g393321v5 CHLRE_17g725950v5

VCN:	VOLCADRAFT_107145 VOLCADRAFT_85926 VOLCADRAFT_92489

» show all

Reference

1 [PMID:10871629]

Authors

Ito Y, Habuchi O.

Title

Purification and characterization of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase from the squid cartilage.

Journal

J Biol Chem 275:34728-36 (2000)
DOI:10.1074/jbc.M909633199

Sequence

[ag:BAB72146]

Reference

2 [PMID:11572857]

Authors

Ohtake S, Ito Y, Fukuta M, Habuchi O

Title

Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene.

Journal

J Biol Chem 276:43894-900 (2001)
DOI:10.1074/jbc.M104922200

Sequence

[hsa:51363] [ag:BAB72146]

Other DBs

ExplorEnz - The Enzyme Database:

2.8.2.33

IUBMB Enzyme Nomenclature:

2.8.2.33

ExPASy - ENZYME nomenclature database:

2.8.2.33

BRENDA, the Enzyme Database:

2.8.2.33

CAS:	242469-38-1

LinkDB

DBGET integrated database retrieval system