KEGG   ENZYME: 3.2.1.165
Entry
EC 3.2.1.165                Enzyme                                 
Name
exo-1,4-beta-D-glucosaminidase;
CsxA;
GlcNase;
exochitosanase;
GlmA;
exo-beta-D-glucosaminidase;
chitosan exo-1,4-beta-D-glucosaminidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
chitosan exo-(1->4)-beta-D-glucosaminidase
Reaction(IUBMB)
Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini [RN:R06225 R08715]
Reaction(KEGG)
R06225(G) R08715 > R01966 R06225(G)
Comment
Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi and comprises D-glucosamine residues with a variable content of GlcNAc residues [4]. Acts specifically on chitooligosaccharides and chitosan, having maximal activity on chitotetraose, chitopentaose and their corresponding alcohols [1]. The enzyme can degrade GlcN-GlcNAc but not GlcNAc-GlcNAc [3]. A member of the glycoside hydrolase family 2 (GH-2) [4].
History
EC 3.2.1.165 created 2008
Pathway
ec00520  Amino sugar and nucleotide sugar metabolism
ec01100  Metabolic pathways
Orthology
K15855  exo-1,4-beta-D-glucosaminidase
Genes
ZJU107408972
NCRNCU09042
NTENEUTE1DRAFT130945(NEUTE1DRAFT_130945)
SMPSMAC_05835
PANPODANSg2615
TTTTHITE_2049779
MTMMYCTH_54601
CTHRCTHT_0049390
MGRMGG_05864
PPEIPpBr36_02687
SSCKSPSK_06994
FGRFGSG_02314
FPUFPSE_03809
FVRFVEG_17613
TRETRIREDRAFT_77299
TRRM419DRAFT_24455
MAWMAC_05140
MAJMAA_09634
CMTCCM_04923
PLJVFPFJ_09576 VFPFJ_10734
ELAUCREL1_5242
PFYPFICI_01243
ANIAN2824.2
AFMAFUA_4G00390
ACTACLA_078510
NFINFIA_045250
AORAO090003001410
ANGANI_1_2638014(An01g06630)
AFVAFLA_000268
ALUCAKAW2_20753S
APUUAPUU_81022S
PCSPc12g13420
PDPPDIP_77980
TMFPMAA_016770
TRGTRUGW13939_11757
UREUREG_02485
ABEARB_07888
TVETRV_00950
PNOSNOG_15490
PTEPTT_07728
BZECOCCADRAFT_5109
BSCCOCSADRAFT_136281
BORCOCMIDRAFT_25729
PFJMYCFIDRAFT_86684
TASAA1Q1_02923
RGLCS053_09885
DJICH75_09920
DTLH8F01_03210
DTADYST_00557
LPYFIV34_02685
LANHKR767_07885
AGEAA314_04805
HPKHprae_1313
MSAMycsm_03422
MYVG155_16065
MYEAB431_16420
MGOAFA91_22240
MDXBTO20_16235
MKUI2456_16720
MGROFZ046_20445
MPAGC0J29_19915
MSENK3U95_13525
MPAEK0O64_15515
MRFMJO55_14580
MCROMI149_16475
MCBMycch_2879
MFTXA26_32930
MLLB1R94_13275
MMAGMMAD_29410
MFXMFAL_08710
MAICMAIC_28430
MIJMINS_27820
MALVMALV_07040
MPSCMPSYJ_35140
MARZMARA_08700
MHEVMHEL_52080
MSARMSAR_39990
MANYMANY_14110
MAUBMAUB_06510
MPHUMPHO_06090
MMUCC1S78_015285
MMATMMAGJ_65440
MBOKMBOE_41550
MFGK6L26_15840
MSEIMSEDJ_43030
SRTSrot_2920
SMASAVERM_1223(csxA)
SVLStrvi_3876
SHYSHJG_2189
SHOSHJGH_1954
SRCM271_06075
SMALSMALA_7195
SSOII1A49_39755
SBHSBI_00935(bga4)
SCIB446_04595
SCXAS200_40475
SRWTUE45_00966(csxA_1)
SGSAVL59_27620
SCADDN051_02655
SAUOBV401_39605
SPUNBFF78_38835
SLXSLAV_03175(csxA)
SRJSRO_6680
SVNCP980_01980
SGFHEP81_07022
SSEOD0Z67_00815
SNFJYK04_00492(csxA)
SBROGQF42_06070
SCHFIPT68_01635
SHUNDWB77_01272(csxA_2)
SCYGS1361_03420(csxA)
SDDD9753_32935
KQIF1D05_31905
AMDAMED_3420 AMED_5067 AMED_6094
AMNRAM_17400 RAM_25805 RAM_31240
AMMAMES_3381 AMES_5007 AMES_6008
AMZB737_3381 B737_5007 B737_6008
AOIAORI_5124
AJAAJAP_13860(csxA)
AMYCCU254_13085 CU254_22695
AMYBBKN51_18055
AMYYYIM_31765(csxA2)
AORISD37_25235
AACDLWP59_02905
APRTMUY14_05400 MUY14_18295
SESPBN6_82410(csxA)
KALKALB_8547 KALB_8660
KPHYAOZ06_13880 AOZ06_38310 AOZ06_47505
LEDBBK82_15950
MTEMGCE86_09470
CAICaci_7497
OCKEXM22_08615
ABAAcid345_1359
ACAACP_1263
TRSTerro_3039
ABASACPOL_4897
ADINH7849_25270
SUSAcid_7527
PFERIRI77_05355
GBAJ421_6020
BCELBcellWH2_01738(csxA_2)
PSACPSM36_0032
PRUPRU_0394
MCOSGM418_25040
ASXCDL62_00075
CBAECOR50_19985
AGIFSB73_19960
ATEEK9M52_18285
PGINFRZ67_21820
SHGSph21_1908
MUCMuYL_2241
MUHHYN43_011735
MGINFRZ54_02725
MRUBDEO27_012770
OLIFKG96_06465
RSIRunsl_2770
RUNDR864_11915
ALSDJ013_10140
ZPRZPR_2497
AALGAREALGSMS7_01260(csxA)
EMARD1013_15210
SHYRLA303_00705
CABYCabys_1956
 » show all
Reference
1  [PMID:2351651]
  Authors
Nanjo F, Katsumi R, Sakai K.
  Title
Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis.
  Journal
J Biol Chem 265:10088-94 (1990)
  Sequence
Reference
2  [PMID:16349528]
  Authors
Nogawa M, Takahashi H, Kashiwagi A, Ohshima K, Okada H, Morikawa Y.
  Title
Purification and Characterization of Exo-beta-d-Glucosaminidase from a Cellulolytic Fungus, Trichoderma reesei PC-3-7.
  Journal
Appl Environ Microbiol 64:890-895 (1998)
DOI:10.1128/AEM.64.3.890-895.1998
  Sequence
Reference
3  [PMID:16877749]
  Authors
Fukamizo T, Fleury A, Cote N, Mitsutomi M, Brzezinski R.
  Title
Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism.
  Journal
Glycobiology 16:1064-72 (2006)
DOI:10.1093/glycob/cwl026
Reference
4  [PMID:16316314]
  Authors
Cote N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R
  Title
Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases.
  Journal
Biochem J 394:675-86 (2006)
DOI:10.1042/BJ20051436
  Sequence
Reference
5  [PMID:16636831]
  Authors
Ike M, Isami K, Tanabe Y, Nogawa M, Ogasawara W, Okada H, Morikawa Y
  Title
Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7.
  Journal
Appl Microbiol Biotechnol 72:687-95 (2006)
DOI:10.1007/s00253-006-0320-y
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.165
IUBMB Enzyme Nomenclature: 3.2.1.165
ExPASy - ENZYME nomenclature database: 3.2.1.165
BRENDA, the Enzyme Database: 3.2.1.165
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