a protopanaxadiol-type ginsenoside with two glycosyl residues at position 20 + H2O = a protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20 + a monosaccharide
Substrate
protopanaxadiol-type ginsenoside with two glycosyl residues at position 20;
H2O [CPD:C00001]
Product
protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20;
monosaccharide
Comment
The 20-O-multi-glycoside ginsenosidase catalyses the hydrolysis of the 20-O-alpha-(1->6)-glycosidic bond and the 20-O-beta-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20, although it can cleave the remaining glucosyl residue with a lower efficiency. Starting with a ginsenoside that is glycosylated at positions 3 and 20, such as ginsenosides Rb1, Rb2, Rb3 and Rc, the most common product is ginsenoside Rd, with a low amount of ginsenoside Rg3 also formed.
History
EC 3.2.1.195 created 2014
Reference
1
Authors
Yu H, Liu Q, Zhang C, Lu M, Fu Y, Im W-T, Lee S-T, Jin F.
Title
A new ginsenosidase from Aspergillus strain hydrolyzing 20-O-multi-glycoside of PPD ginsenoside.
