Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
type II arabinogalactan exo alpha-(1,3)-[beta-L-arabinopyranosyl-(1->3)-L-arabinofuranose] hydrolase (non-reducing end)
Reaction(IUBMB)
Hydrolysis of beta-L-Arap-(1->3)-L-Araf disaccharides from non-reducing terminals in branches of type II arabinogalactan attached to proteins.
Comment
The enzyme, characterized from the bacterium Bifidobacterium pseudocatenulatum, specifically hydrolyses beta;-L-Arap-(1->3)-L-Araf disaccharides from the non-reducing terminal of arabinogalactan using an exo mode of action. It is active with arabinogalactan-proteins (AGPs) containing type II arabinogalactans such as gum arabic AGP and larch AGP. The enzyme can also hydrolyse alpha-D-Galp-(1->3)-L-Araf disaccharides (cf. EC 3.2.1.215) with a much lower activity.
History
EC 3.2.1.223 created 2023
Reference
1
Authors
Sasaki, Y., Yanagita, M., Hashiguchi, M., Horigome, A., Xiao, J. Z., Odamaki, T., Kitahara, K. and Fujita, K.
Title
Assimilation of arabinogalactan side chains with novel 3-O-beta-L-arabinopyranosyl-alpha-L-arabinofuranosidase in Bifidobacterium pseudocatenulatum.