KEGG   ENZYME: 3.2.2.1
Entry
EC 3.2.2.1                  Enzyme                                 
Name
purine nucleosidase;
nucleosidase (misleading);
purine beta-ribosidase;
purine nucleoside hydrolase;
purine ribonucleosidase;
ribonucleoside hydrolase (misleading);
nucleoside hydrolase (misleading);
N-ribosyl purine ribohydrolase;
nucleosidase g;
N-D-ribosylpurine ribohydrolase;
inosine-adenosine-guanosine preferring nucleoside hydrolase;
purine-specific nucleoside N-ribohydrolase;
IAG-nucleoside hydrolase;
IAG-NH
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
Sysname
purine-nucleoside ribohydrolase
Reaction(IUBMB)
a purine nucleoside + H2O = D-ribose + a purine base [RN:R02341]
Reaction(KEGG)
Substrate
purine nucleoside [CPD:C15586];
H2O [CPD:C00001]
Product
D-ribose [CPD:C00121];
purine base [CPD:C15587]
Comment
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [6].
History
EC 3.2.2.1 created 1961, modified 2006, modified 2011
Pathway
ec00230  Purine metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K01239  purine nucleosidase
Genes
TBRTb927.3.2960
TBGTbgDal_III3040
TCR508153.640 510315.10
LMALMJF_29_2800
LIFLINJ_29_2910
LDOLDBPK_292910
LMILMXM_08_29_2800
LBZLBRM_29_2850
LPANLPMP_292810
PAZOAYR47_21590
PFWPF1751_v1c24650
PFFPFLUOLIPICF724405
PFXA7318_11855
PLULFOB45_04345
PANRA7J50_2711
PYMAK972_2669
PVWHU752_024340
GAIIMCC3135_31495(rihB_2)
BUGBC1001_5650
BGFBC1003_5748
BGDbgla_1g02480
BGOBM43_1629
BUQAC233_30665
BUMAXG89_38745
BUIAX768_21595
BGEBC1002_3730
BPXBUPH_06110
BPYBphyt_4161 Bphyt_4926
BFNOI25_4158 OI25_6672
BCAIK788_0001662
PSPWBJG93_16935
PHSC2L64_38500
PTERC2L65_34500
PGPCUJ91_25535
PCJCUJ87_19760
PTSCUJ90_18535
PMEGFNZ07_10215 FNZ07_17155
PGISI6I06_21330
PEWKZJ38_18480
PDIOPDMSB3_2824.1
BUOBRPE64_CCDS00930
RHYRD110_00760
VAPVapar_0152
VPEVarpa_0177
VPDVAPA_1c01850
VBOCKY39_00965
VAMC4F17_18090
CARELT85_4668
MESPC1M53_29500
OPSA8A54_20110
OCHCES85_4910
LNEFZC33_25635
BANBA_2888 BA_3606 BA_5338
BARGBAA_2888 GBAA_3606 GBAA_5338
BATBAS2693 BAS3345 BAS4961
BAHBAMEG_1024 BAMEG_1711 BAMEG_5393
BAIBAA_2947 BAA_3635 BAA_5370
BAXH9401_2756 H9401_3429 H9401_5093
BANTA16_29180 A16_36130 A16_53530
BANRA16R_29630 A16R_36590 A16R_54160
BANSBAPAT_2774 BAPAT_3454 BAPAT_5120
BANHHYU01_14310 HYU01_17645 HYU01_26095
BANVDJ46_1658 DJ46_2326 DJ46_4006
BCEBC2889 BC3552 BC5134
BCABCE_2927 BCE_3566 BCE_5237(iunH) BCE_A0076
BCZBCE33L2619(iunH) BCE33L3260 BCE33L4823
BCRBCAH187_A2939 BCAH187_A3569 BCAH187_A5283 BCAH187_C0090
BCBBCB4264_A2614 BCB4264_A2904 BCB4264_A3659 BCB4264_A5248
BCUBCAH820_2891 BCAH820_3559 BCAH820_5219 BCAH820_B0062
BCGBCG9842_B1658 BCG9842_B2170 BCG9842_B2341 BCG9842_B5704
BCQBCQ_2726(iunH) BCQ_3345 BCQ_4939
BCXBCA_2968 BCA_3645 BCA_5245
BALBACI_c28560(iunH) BACI_c34950 BACI_c51160
BNCBCN_2749 BCN_3354 BCN_5032 BCN_P075
BCFbcf_14130 bcf_17600 bcf_25600 bcf_28005
BCERBCK_09730 BCK_17505 BCK_20430
BCEFBcrFT9_04110
BCYBcer98_1846 Bcer98_1982 Bcer98_2226
BTKBT9727_2642(iunH) BT9727_3310 BT9727_4813
BTLBALH_2595(iunH) BALH_3191(iunH) BALH_4626(iunH)
BTBBMB171_C2593 BMB171_C3226 BMB171_C4706
BTTHD73_3089 HD73_3823 HD73_5466
BTHRYBT1520_15215 YBT1520_18865 YBT1520_27110
BTHIBTK_15315 BTK_18805 BTK_27080
BTCCT43_CH2882 CT43_CH3495 CT43_CH3774 CT43_CH5162
BTFYBT020_14445 YBT020_17390 YBT020_25350
BTMMC28_2078 MC28_2700 MC28_G096
BTGBTB_c30070(rihA2) BTB_c36270(rihA3) BTB_c39030(rihA4) BTB_c53250(rihA5)
BTIBTG_01625 BTG_04035 BTG_04905 BTG_23065
BTNBTF1_11970 BTF1_12770 BTF1_15510 BTF1_24055
BTHTH175_ch2930 H175_ch3553 H175_ch3834 H175_ch5244
BTHUYBT1518_15920 YBT1518_19480 YBT1518_28570
BTWBF38_4050 BF38_4725 BF38_862
BTHYAQ980_03960 AQ980_12205 AQ980_15705
BWEBcerKBAB4_1745 BcerKBAB4_2695 BcerKBAB4_3254 BcerKBAB4_4926
BWWbwei_1388(iunH) bwei_2135(iunH) bwei_3142(iunH) bwei_4664(iunH)
BMYOBG05_2460 BG05_3131 BG05_4102 BG05_924
BTYBtoyo_0175 Btoyo_0764 Btoyo_2335 Btoyo_4749
BMYCDJ92_5166 DJ92_533
BBYCY96_13240 CY96_16750 CY96_24665 CY96_29130
BWDCT694_15405 CT694_18890 CT694_27620 CT694_30660
BTROFJR70_04690 FJR70_10650 FJR70_19765
BMOBMLA2C4_15290 MLA2C4_18435
BNTGSN03_16790 GSN03_24390
BPACLMD38_01330 LMD38_09375 LMD38_12655
BPANNLJ82_13605 NLJ82_16765 NLJ82_25175 NLJ82_28460
BIFN288_15890 N288_16315
BWHA9C19_08040
BACQDOE78_10895
BACAFAY30_15120
BHKB4U37_04505 B4U37_08015 B4U37_12185
BCOHBC6307_09290 BC6307_10885
BFXBC359_12685
PKSIE339_08545
BHABH0417
BCLABC3550 ABC3998
BLEBleG1_3873
BPFBpOF4_09550 BpOF4_19070
BONA361_11340 A361_18380 A361_18780
CGOTJ1899_13560
CSUAIM538_06475
CFIRNAF01_03285 NAF01_16490 NAF01_16900
CSOALIS82_17520
OIHOB2433
LSPBsph_2870
LFUHR49_12380
LYSLBYS11_11080
LYGC1N55_11155
LPAKGDS87_12380
LYCFH508_0010815
LCAPICJ70_12575
HMNHM131_20230
HLIHLI_03225 HLI_04125 HLI_05245
VIRX953_13785
VHLBME96_13150
VIGBKP57_17960
VILCFK37_11700
VNECFK40_06945
VPTKBP50_11760
LAOAOX59_18630
FPNABE65_019585
FARABE41_003215 ABE41_005680 ABE41_018645
PSYHD0S48_04650
POFGS400_09900
MEKUHUW50_25725
MSEMGMB29_15990
MDGK8L98_05635 K8L98_07305
MLITKDJ21_015255
BVQFHE72_03630 FHE72_13490 FHE72_22770
RMFD5E69_08865
BVJI5776_09540
BOUI5818_06375 I5818_10850
MCUIG8O30_06410 G8O30_11825
GAJMY490_08685
MJOFOF60_12610 FOF60_15450
SPSYAZE41_14320
AFNAcfer_0833
LPILLIP_2236
MTURv3393(iunH)
MTVRVBD_3393
MTCMT3500
MRAMRA_3432(iunH)
MTFTBFG_13427
MTBTBMG_03443
MTKTBSG_03465
MTZTBXG_003418
MTGMRGA327_20945
MTIMRGA423_21380
MTURCFBS_3596(iunH)
MTLCCDC5180_3091
MTOMTCTRI2_3464(iunH)
MTDUDA_3393(iunH)
MTNERDMAN_3712(iunH)
MTJJ112_18260
MTUBMT7199_3439
MTUEJ114_18180
MTXM943_17490
MTUHI917_23845
MTULTBHG_03329
MTUTHKBT1_3583(iunH)
MTUUHKBT2_3590(iunH)
MTQHKBS1_3593(iunH)
MBOBQ2027_MB3425(iunH)
MBBBCG_3462(iunH)
MBTJTY_3462(iunH)
MBMBCGMEX_3460(iunH)
MBKK60_035300
MBXBCGT_3265
MAFMAF_34060(iunH)
MMICRN08_3743
MCEMCAN_34161(iunH)
MCQBN44_80050(iunH)
MCVBN43_70045(iunH)
MCXBN42_50050(iunH)
MCZBN45_70035(iunH)
MPAMAP_3484(iunH)
MAOMAP4_0293
MAVIRC58_01385
MAVURE97_01395
MAVMAV_4342
MITOCO_42070
MIAOCU_41990
MIDMIP_06341
MYOOEM_42420
MCHIAN480_22060
MIROCQ_43340
MMALCKJ54_20105
MMANMMAN_30170
MSAMycsm_01150
MMIMMAR_1152(iunH)
MMAEMMARE11_11120(iunH)
MLIMULP_01294(iunH)
MPSEMPSD_12970(iunH)
MSHOMSHO_29870(iunH)
MMCMmcs_1178
MKMMkms_1195
MJLMjls_1205
MMMW7S_21045
MKNMKAN_20405
MYVG155_08255
MYEAB431_07075
MGOAFA91_30820
MHADB586_18110
MDXBTO20_27260
MSHGMSG_01054
MFJMFLOJ_23310
MSTOMSTO_27270
MSIMMSIM_14490
MSAKMSAS_46650(iunH)
MKUI2456_05875
MGROFZ046_12020
MXEMYXE_38400
MNVMNVI_26860
MPAGC0J29_06430
MNMMNVM_28550(iunH)
MGORH0P51_05720
MCOOMCOO_43490
MBAIMB901379_01017(rihA)
MSEOMSEO_49850
MHEKJMUB5695_03772
MGAUMGALJ_56450
MLJMLAC_33430(iunH)
MBRDMBRA_04900
MSHJMSHI_26270
MKRMKOR_21510
MDFK0O62_07150
MMAMK3U93_04945
MHOLK3U96_20210
MHERK3U94_18095
MSENK3U95_07645
MPAEK0O64_06730
MSPGF6B93_04970
MOTLTS72_19810
MVMMJO54_18265
MRFMJO55_06260
MPAAMKK62_19280
MCROMI149_07540
MSMMSMEG_1621
MSGMSMEI_1584(iunH)
MSBLJ00_08105
MSNLI99_08105
MSHLI98_08105
MVAMvan_1526
MGIMflv_4897
MSPMspyr1_43140
MCBMycch_1128
MNED174_07585
MYNMyAD_07450
MFTXA26_16460
MPHLMPHLCCUG_01462(rihA)
MVQMYVA_1278
MLLB1R94_22015
MRHMycrhN_0547
MTHN4412656_01075(rihA)
MHASMHAS_00393(rihA)
MDUMDUV_32470
MCHTMCHIJ_51880
MDRMDOR_07650
MAUUNCTC10437_01243(rihA)
MMAGMMAD_12780
MMORMMOR_52670
MFXMFAL_19940
MAICMAIC_11500
MIJMINS_15130
MALVMALV_22620
MTYMTOK_33680
MPSCMPSYJ_52160
MARZMARA_26420
MGADMGAD_26440
MHEVMHEL_34730
MSARMSAR_05610
MANYMANY_51150
MAUBMAUB_19860
MPOFMPOR_17700
MPHUMPHO_50650
MMUCC1S78_021905
MMATMMAGJ_44930
MBOKMBOE_59730
MFGK6L26_24405
MSEIMSEDJ_25240
MFLVNCTC10271_03983(rihA)
MCEEMCEL_21810
MBRML2Z93_003096
MABMAB_3711
MMVMYCMA_2039
MABBMASS_3723
MABLMMASJCM_3711
MCHEBB28_18970
MIZBAB75_21195
MSTEMSTE_03860
MSAOMYCSP_17125
MSALDSM43276_03509(rihA)
MJDJDM601_3215(iunH)
MTER4434518_03202(iunH)
MHIBMHIB_26240(iunH)
ASDAS9A_1939(iunH)
MPAKMIU77_14580
CGLCgl2835(Cgl2835)
CGBcg3137(iunH1)
CGUWA5_2736(IunH1)
CGTcgR_2720
CGSC624_13875
CGGC629_13880
CGMcgp_3137(iunH1)
CGJAR0_13750
CGQCGLAR1_13615
CGXSB89_13345
CDIDIP2340
CDPCD241_2221
CDHCDB402_2199
CDTCDHC01_2220
CDECDHC02_2205
CDRCDHC03_2229
CDACDHC04_2240
CDZCD31A_2362
CDBCDBH8_2336
CDSCDC7B_2313
CDDCDCE8392_2228
CDWCDPW8_2320
CDVCDVA01_2156
CDIPERS451417_02365(rihA_2)
CJKjk0233(iunH1)
CURcu1768
CUACU7111_1706(iunH1)
CKPckrop_1727(iunH)
CRDCRES_0279(iunH)
CVACVAR_0416
CCNH924_11900
CTERA606_01585
CMDB841_05415
CFNCFAL_01415
CCGCCASEI_00855
CVTB843_05465
CGYCGLY_14075
CIICIMIT_04660
CUVCUREI_04815
COADR71_857
CSXCSING_06760(iunH1)
CMQB840_04705
CEICEPID_01635
CLWCLAC_11120
CDXCDES_12700
CSPWM42_0128
CSTACSTAT_00805
CCJZccrud_12775
CPHOCPHO_05930
CFCCFLV_06515
CGVCGLAU_05025(rihA2)
CSTRCBE89_07390
CPEGCPELA_09695(rihA3)
CXEFOB82_04495
CSANE3227_10650
CGKCGERO_09465(rihA3)
CRFFRC0190_02373
CPRECsp1_23270(rihA_2)
CPSOCPPEL_09885(rihA3)
CSURN24_2924
CMATHBA49_11635
CKFI6I12_06560
CANSGP473_01125
CLIAC3E79_04340
CPRPI6I69_07025
CAMCI6I65_06505
CUOCUROG_01475(rihC)
CLIZG7Y31_06035
CTUBI6I74_11255
CLUJIAU68_04675
CQNG7Y29_04150
CCOECETAM_05135(rihA1)
CZOIAU67_01200
CCYCSCMU_18750
CORYFQV43_03620
CMFGWO64_005980
CWKIA203_05150
CUWLH390_04405
CPOYGP475_01195
CACCCACC_06560(rihA1)
CFYI6L56_07625
BFVC628_14095
NFANFA_25490
NFRERS450000_01547(rihA)
NCYNOCYR_2644
NBRO3I_019740
NNONONO_c40980
NSLBOX37_16780
NSRNS506_04843(iunH)
NTPCRH09_21295
NOZDMB37_40425
NODFOH10_14460
NAHF5544_20940
NADNCTC11293_03830(rihA)
NWLNWFMUON74_35490
NIEKV110_20170
NTCKHQ06_26450
NHUH0264_24720
NYALTV02_09305
NGPLTT66_35665
RHARHA1_ro07236(iunH)
RERRER_30920
REYO5Y_14180
REBXU06_14395
RQIC1M55_15420
ROPROP_70200
ROAPd630_LPD03983
RPYY013_13695
RBYCEJ39_01075
RHBNY08_1054
RAVAAT18_04865
RFAA3L23_04302(rihA)
RHSA3Q41_03964(rihA)
RHWBFN03_07735
RRZCS378_20400
RHUA3Q40_01692(rihA)
RHQIM25_05040
RHODAOT96_25625
RRT4535765_04668(iunH)
RCRNCTC10994_02838(iunH)
RTMG4H71_21315
RKOJWS14_37870
RGOKYT97_03725
ROZCBI38_15355
RPSKJWS13_06355
REQREQ_22710
GBRGbro_2431
GPOGPOL_c23670
GORKTR9_2339
GTABCM27_12465
GRUGCWB2_13200(rihA)
GOMD7316_00967(rihA)
GAVC5O27_14885
GODGKZ92_11965
GAMGII34_12005
GPDGII33_11330
GJIH1R19_11665
GOILK459_21650
GHNMVF96_11875
GAMIIHQ52_15700
GMGNWF22_22190
SPINKV203_10075
TPRTpau_2122
TSMASU32_11545
TPULTPB0596_24670
SRTSrot_0415 Srot_0615
DITC3V38_13820
DIZCT688_08005
DPCA6048_08265
DLUA6035_07855
DKNNHB83_07565
SSUBCP968_33480
CHREIE160_10090
ARTArth_1650
ARLAFL94_15755
ARYATC04_02190
ARUASPU41_13535
ARQBWQ92_01710
ACRYAC20117_17675
ARTHC3B78_07825
ARTPE5206_15215
ACITHPK19_24805
AAGINCTC2676_1_00554(rihA)
ARTYAHiyo4_20190
ARIUM93_10245
ACHAchl_1642
APNAsphe3_16230
PSULAU252_02165
PSNINIBR502771_17280
AAIAARI_31690
GARAOZ07_15905
GLUF0M17_15145
GPRJQN66_15745
GMINMP99_15695
GMYXH9_10105
RSARSal33209_0933
MLUMlut_06650
MICKB1A86_00003215
MYUM8233_03060
RTERIDM49_09705
RAMAIDM48_09450
SATKSA2016_1883
PAEYKUF55_06980
BFABfae_23440
BRXBH708_08245
BRVCFK39_04090
BGGCFK41_04800
BRZCFK38_07055
BSAUDWV08_09205
BRRC1N80_07265
BKIM4486_00265
BRHANLU66_07915
BHHBra3105_16115
PSEIGCE65_01600
BLYA2T55_07455
BLINBLSMQ_1587
BCAUI6G59_10575
PACPPA2076
PAKHMPREF0675_5127
PAVTIA2EST22_10080
PAXTIA2EST36_10070
PAZTIA2EST2_10025
PAWPAZ_c21490(rihA)
PADTIIST44_03075
PCNTIB1ST10_10540
PACCPAC1_10510
PACHPAGK_1972
PACNTIA1EST1_10165
CACNRN83_10600
PRAPALO_00695
PRLBCB70_08020
CGRN4412665_00787(rihA)
PFRPFREUD_19310(iunH)
PFRERM25_1839
PACDEGX94_08705
PAUSNCTC13651_02109(rihA_1)
MIKFOE78_01630
MICGGJV80_17250
MGGMPLG2_0284(rihA)
CWOCwoe_3809
SUSAcid_1342
CPORBED41_11800
TOHBCB71_10595
 » show all
Reference
1  [PMID:12999785]
  Authors
HEPPEL LA, HILMOE RJ.
  Title
[Phosphorolysis and hydrolysis of purine ribosides by enzymes from yeast.]
  Journal
J Biol Chem 198:683-94 (1952)
Reference
2
  Authors
Kalckar, H.M.
  Title
Biosynthetic aspects of nucleosides and nucleic acids.
  Journal
Pubbl Staz Zool (Napoli) 23:87-103 (1951)
Reference
3  [PMID:13416219]
  Authors
TAKAGI Y, HORECKER BL.
  Title
Purification and properties of a bacterial riboside hydrolase.
  Journal
J Biol Chem 225:77-86 (1957)
Reference
4  [PMID:14363106]
  Authors
TARR HL.
  Title
Fish muscle riboside hydrolases.
  Journal
Biochem J 59:386-91 (1955)
DOI:10.1042/bj0590386
Reference
5  [PMID:8702965]
  Authors
Parkin DW.
  Title
Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei. Purification, specificity, and kinetic mechanism.
  Journal
J Biol Chem 271:21713-9 (1996)
DOI:10.1074/jbc.271.36.21713
  Sequence
Reference
6  [PMID:11282633]
  Authors
Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu S.
  Title
Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi.
  Journal
Appl Environ Microbiol 67:1783-7 (2001)
DOI:10.1128/AEM.67.4.1783-1787.2001
Reference
7  [PMID:11854281]
  Authors
Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J.
  Title
Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.
  Journal
J Biol Chem 277:15938-46 (2002)
DOI:10.1074/jbc.M111735200
  Sequence
Reference
8  [PMID:15909995]
  Authors
Mazumder-Shivakumar D, Bruice TC.
  Title
Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues.
  Journal
Biochemistry 44:7805-17 (2005)
DOI:10.1021/bi047394h
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.1
IUBMB Enzyme Nomenclature: 3.2.2.1
ExPASy - ENZYME nomenclature database: 3.2.2.1
BRENDA, the Enzyme Database: 3.2.2.1
CAS: 9025-44-9
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