KEGG   ENZYME: 3.2.2.8
Entry
EC 3.2.2.8                  Enzyme                                 
Name
ribosylpyrimidine nucleosidase;
N-ribosylpyrimidine nucleosidase;
pyrimidine nucleosidase;
N-ribosylpyrimidine ribohydrolase;
pyrimidine nucleoside hydrolase;
RihB;
YeiK;
nucleoside ribohydrolase
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
Sysname
pyrimidine-nucleoside ribohydrolase
Reaction(IUBMB)
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base [RN:R02172]
Reaction(KEGG)
R02172 > R01080 R02137
Substrate
pyrimidine nucleoside [CPD:C03169];
H2O [CPD:C00001]
Product
D-ribose [CPD:C00121];
pyrimidine base [CPD:C00396]
Comment
Also hydrolyses purine D-ribonucleosides, but much more slowly. 2'-, 3'- and 5'-deoxynucleosides are not substrates [3].
History
EC 3.2.2.8 created 1972
Pathway
ec00240  Pyrimidine metabolism
ec01100  Metabolic pathways
Orthology
K10213  ribosylpyrimidine nucleosidase
Genes
ECOb2162(rihB)
ECJJW2149(rihB)
ECDECDH10B_2319(rihB)
EBWBWG_1944(rihB)
ECOKECMDS42_1731(rihB)
ECEZ3419(yeiK)
ECSECs_3054(rihB)
ECFECH74115_3298
ETWECSP_3040(rihB)
ELXCDCO157_2817(rihB)
EOIECO111_2880(rihB)
EOJECO26_3074(rihB)
EOHECO103_2637(rihB)
ECOOECRM13514_2924(rihB)
ECOHECRM13516_2864(rihB)
ESLO3K_08635(rihB)
ESOO3O_17000(rihB)
ESMO3M_08585(rihB)
ECKEC55989_2415(rihB)
ECGE2348C_2308(rihB)
EOKG2583_2705(rihB)
ELRECO55CA74_13375(rihB)
ELHETEC_2297
ECWEcE24377A_2459
EUNUMNK88_2707
ECPECP_2202
ENAECNA114_2253(yeiK)
ECOSEC958_2498(yeiK)
ECVAPECO1_43912(rihB)
ECOAAPECO78_14530(rihB)
ECXEcHS_A2298
ECMEcSMS35_2309
ECYECSE_2430
ECRECIAI1_2242(rihB)
ECQECED1_2610(rihB)
EUMECUMN_2498(rihB)
ECTECIAI39_2302(rihB)
EOCCE10_2534(rihB)
EBRECB_02091(rihB)
EBLECD_02091(rihB)
EBEB21_02050(rihB)
EBDECBD_1496
ECIUTI89_C2436(yeiK)
EIHECOK1_2394
ECZECS88_2310(rihB)
ECCc2696(yeiK)
ELOEC042_2395(rihB)
ELNNRG857_10975(rihB)
ESEECSF_2043
ECLEcolC_1486
EKOEKO11_1592
EKFKO11_11820(rihB)
EABECABU_c24930(rihB)
EDHEcDH1_1496
EDJECDH1ME8569_2098(rihB)
ELUUM146_05985(rihB)
ELWECW_m2363(rihB)
ELLWFL_11560(rihB)
ELCi14_2497(rihB)
ELDi02_2497(rihB)
ELPP12B_c2256(rihB)
ELFLF82_1884(rihB)
ECOLLY180_11300(rihB)
ECOIECOPMV1_02322(rihB)
ECOJP423_12155(rihB)
EFEEFER_2248(rihB)
EMAC1192_01120(rihB)
SESSARI_00683
SFLSF2247(rihB)
SFXS2376(yeiK)
SFVSFV_2237(yeiK)
SFESFxv_2480(rihB)
SFNSFy_3191
SFSSFyv_3266
SFTNCTC1_02470(rihB)
SSNSSON_2218(yeiK)
SBOSBO_2165(yeiK)
SBCSbBS512_E0804
SDYSDY_2104(yeiK)
SDZAsd1617_02833
SHQA0259_14630(rihB)
KLWDA718_08895
RTGNCTC13098_05754(rihB)
LPNUKQ929_06395(rihB)
EAMEGXP68_16060(rihB)
HAVAT03_11825(rihB)
OPODSM2777_15880(rihB)
LRINCTC12151_01991(rihB)
BFDNCTC4823_01338(rihB_1)
COHNKCTCHS21_58260
CCHLFPL14_10140
LBRLVIS_2188
LBKLVISKB_2248
LROSLROSL1_0565
EGVEGCR1_13640
EAVEH197_07985
CEUA7L45_04060(rihB)
OVAOBV_11660(rihB)
LACYA4V08_10600(rihB)
CSHClosa_3276
LBWC3V36_05240(rihB)
GEKkuro4_06140
TBKHF295_08070
BDEBDP_1920(iunH)
BDNBBDE_1812
BLEMBL8807_02170
BEUBE0216_09410
KBSEPA93_42270
SASSMUG09_05315
BRMBmur_2117
BPJB2904_orf2394
BPWWESB_0409
LBALebu_1571
LEOAMK43_06435(rihB)
STRSterm_3626 Sterm_3734
TAITaci_0564
 » show all
Reference
1  [PMID:12325375]
  Authors
Terada M, Tatibana M, Hayaishi O.
  Title
Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens.
  Journal
J Biol Chem 242:5578-85 (1967)
Reference
2  [PMID:11027694]
  Authors
Petersen C, Moller LB
  Title
The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation.
  Journal
J Biol Chem 276:884-94 (2001)
DOI:10.1074/jbc.M008300200
  Sequence
[eco:b2162]
Reference
3  [PMID:14993681]
  Authors
Giabbai B, Degano M.
  Title
Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK.
  Journal
Acta Crystallogr D Biol Crystallogr 60:524-7 (2004)
DOI:10.1107/S0907444903028488
Reference
4  [PMID:15130467]
  Authors
Giabbai B, Degano M.
  Title
Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy.
  Journal
Structure 12:739-49 (2004)
DOI:10.1016/j.str.2004.03.018
  Sequence
[eco:b2162]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.8
IUBMB Enzyme Nomenclature: 3.2.2.8
ExPASy - ENZYME nomenclature database: 3.2.2.8
BRENDA, the Enzyme Database: 3.2.2.8
CAS: 37288-60-1
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