Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases
BRITE hierarchy

(1) glutathione + H2O = L-cysteinylglycine + L-glutamate [RN:R00494];
(2) a glutathione-S-conjugate + H2O = an (L-cysteinylglycine)-S-conjugate + L-glutamate

R00494
Reaction

glutathione [CPD:C00051];
H2O [CPD:C00001];
glutathione-S-conjugate

This is a bifunctional protein that also has the activity of EC 2.3.2.2, gamma-glutamyltransferase. The enzyme binds its substrate by forming an initial gamma-glutamyl-enzyme intermediate, releasing the L-cysteinylglycine part of the molecule. The enzyme then reacts with either a water molecule or a different acceptor substrate (usually an L-amino acid or a dipeptide) to form L-glutamate or a product containing a new gamma-glutamyl isopeptide bond, respectively. The enzyme acts on glutathione, glutathione-S-conjugates, and, at a lower level, on other substrates with an N-terminal L-gamma-glutamyl residue. It plays a crucial part in the glutathione-mediated xenobiotic detoxification pathway. The enzyme consists of two chains that are created by the proteolytic cleavage of a single precursor polypeptide.

EC 3.4.19.13 created 2011, modified 2019