KEGG   ENZYME: 3.4.19.16
Entry
EC 3.4.19.16                Enzyme                                 
Name
glucosinolate gamma-glutamyl hydrolase;
GGP1 (gene name);
GGP3 (gene name)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases
Reaction(IUBMB)
(1) an (E)-1-(glutathion-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine + H2O = an (E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine + L-glutamate [RN:R11792];
(2) (E)-1-(glutathion-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine + H2O = (E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine + L-glutamate [RN:R11793];
(3) (glutathion-S-yl)(1H-indol-3-yl)acetonitrile + H2O = (L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile + L-glutamate [RN:R11794];
(4) (Z)-1-(glutathion-S-yl)-N-hydroxy-2-phenylethan-1-imine + H2O = (Z)-1-(L-cysteinyglycin-S-yl)-N-hydroxy-2-phenylethan-1-imine + L-glutamate [RN:R11795]
Reaction(KEGG)
Substrate
(E)-1-(glutathion-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine;
H2O [CPD:C00001];
(E)-1-(glutathion-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine;
(glutathion-S-yl)(1H-indol-3-yl)acetonitrile;
(Z)-1-(glutathion-S-yl)-N-hydroxy-2-phenylethan-1-imine
Product
(E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-omega-(methylsulfanyl)alkan-1-imine;
L-glutamate [CPD:C00025];
(E)-1-(L-cysteinylglycin-S-yl)-N-hydroxy-2-(1H-indol-3-yl)ethan-1-imine;
(L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile;
(Z)-1-(L-cysteinyglycin-S-yl)-N-hydroxy-2-phenylethan-1-imine
Comment
This enzyme, characterized from the plant Arabidopsis thaliana, participates in the biosynthesis of the plant defense compounds glucosinolates and camalexin. It is the only known plant enzyme capable of hydrolysing the gamma-glutamyl residue of glutathione in the cytosol.
History
EC 3.4.19.16 created 2017
Orthology
K22314  glucosinolate gamma-glutamyl hydrolase
Genes
NVE5515324
EPA110235103 110236718 110255075
ATEN116299149
ADF107348401
AMIL114964847
PDAM113677131
SPIS111327171
DGT114526438
XEN124440256
HMG101240492 105844229
HSY130612435 130622308 130623845 130642202
RES135694783 135694829 135695003
ATHAT2G23960 AT2G23970 AT4G30530(GGP1) AT4G30540 AT4G30550(GGP3)
ALY9303414 9305443 9305444 9316603
CRB17878381 17878489 17880012 17880488 17890112
CSAT104702915 104713836 104717139 104717141 104717142 104721790 104721793 104721794 104730255 104730257 104730258 104752025 104774366
EUSEUTSA_v10000299mg EUTSA_v10000640mg EUTSA_v10026067mg EUTSA_v10026083mg EUTSA_v10027000mg
BRP103834667 103834668 103842392 103842394 103852592 103861974
BNA106365159 106368635 106392894 106400381 106408952 106414873 106416083 106424103 106424211 125583484 125583485 125590147 125609478
BOE106306103 106306562 106310578 106312467 106329223 106329266
RSZ108809618 108813611 108816757 108831369 108833915 108833916 108851551 130501945 130504970 130505449 130505658 130508167
THJ104803534 104803536 104803539 104803921 104807184 104819995
CPAP110819018
CIT102624397 102629214
CICCICLE_v10009284mg CICLE_v10029932mg
PVY116121936 116121937 116142064 116143811
MINC123194511 123195200 123199885 123201146 123201149 123213117
TCC18589037 18608189
GRA105779911 105782861 105791012 105795123
GHI107886999 107894173 107898771 107907726 107920347 107945175 121204599 121225719
GAB108459694 108461703 108474421 108482468
HSYR120115880 120116496 120120593 120134194 120145170 120149827 120171611 120177480 120177953 120210568 120212013 120214909
DZI111282511 111282881 111285067 111295545 111304248 111309066
EGR104419874 104419875 104436013 104436014
GMX100777176 100794668 100808286 100813163
GSJ114375643 114393199 114419424 114422977
PVUPHAVU_002G214100g PHAVU_003G153000g PHAVU_006G201200g
VRA106767438 106767996 106776292
VAR108320294 108341076 108341427
VUN114175230 114179829 114187141 114188220
VUM124823192 124831135 124836648
CCAJ109803173 109804642
APRC113847132 113852959 113859095
MTR11407738 11431148 11433871
TPRA123910556 123911308 123912780 123922353
CAM101488889 101497879 101509568 101509890
PSAT127084034 127106184
VVO131604850 131624892 131631347 131633881
LJALj4g3v0386190.1(Lj4g3v0386190.1) Lj4g3v2016180.1(Lj4g3v2016180.1) Lj6g3v2192310.1(Lj6g3v2192310.1)
ADU107459585 107468739 107480452
AIP107624114 107631057 107641548
AHF112699490 112714793 112723474 112733382 112784254 112791316
LANG109326195 109329052 109332015 109334535 109349298
PCIN129285814 129305370 129306780 129307425 129307877
QSAO6P43_012441 O6P43_022235 O6P43_031678
FVE101306191 101310360
RCN112171161 112200289
PPER18767070 18792319
PMUM103323428 103335981
PAVI110759102 110764109
PDUL117612402 117615658
MDM103412048 103428242 114822053 114824755
MSYL126585490 126597077 126607360 126620703
PXB103929232 103935733 103942059 103950509 103956111 103957818
ZJU107425741 107426808
MNT21391927 21397902
CSAV115694907 115719792 115724737
CSV101205790 101206066 101215620
CMO103494858 103495371
BHJ120077669 120091938
MCHA111007348 111008451 111017631
CMAX111470593 111482610 111487691
CMOS111430531 111448037 111455260
CPEP111787978 111810368 111811902
RCU8265758 8279073
JCU105633567 105633617 105641450
HBR110635404 110638732 110647629 110648296
MESC110616952 110618732 110623930
POP7454830 7493370
PEU105110012 105121222
PALZ118027613 118034263
JRE108983914 108989512 108993096 108995673
CILL122297206 122299526 122309085 122312454
CAVE132175378 132178807 132185614
QSU112009412 112037074
QLO115958950 115967436
TWL119982074 119984548 119993286
VVI100259085 100267393
VRI117910103 117911279 117925440
SLY101254399 101267066
SPEN107009125 107025647
SOT102585222 102585501
SSTN125850894 125877659
SDUL129885711 129893111
CANN107840110 107854225
LBB132598855 132602901
NTA107765602 107772668 107794450 107809941 107830013
NSY104212375 104218893 104249304
NTO104110542 104111543
NAU109221059 109229136
INI109161240 109165058 109166351
ITR116026325 116028479 116033482
SIND105155619 105169292 105172639
OEU111374295 111374296 111380734 111386638 111389020 111399606 111410202 111410677
EGT105949599 105956101 105958351
SSPL121755386 121758298 121803355 121806373
SMIL131005034 131005171
SHIS125193226 125223422
APAN127251472 127253509 127262449 127264719
HAN110917519 110929502 110940260
ECAD122593758 122594670 122598474 122600190
LSV111899051 111906921 111913274
CCAV112500408 112507411 112517466
DCR108211617 108221955 108225446
CSIN114282624 114288256 114296361
RVL131308059 131320358 131325453
AEW130752680 130754956 130777397 130778972
BVG104901749 104904768
SOE110801426 110805905
CQI110685084 110715792 110721737 110740064
ATRI130797458 130806638
MOF131157351 131157609 131157721 131157965 131164769
NNU104589638 104589829 104589830 104589831 104590921 104595528 104605395 104605396 104605398 104605399 104605400 104605401 104605402 104605403 104611953
MING122071598 122071696 122073589 122074705 122075703 122080199 122080785 122080838 122080883 122083572 122088717 122089695
TSS122644724 122657645 122659748 122660540 122660541 122661131 122662310 122662381 122662395 122663021
PSOM113349692 113357469
OSA4328499 4333748 4347102
DOSAOs02t0179200-01(Os02g0179200) Os03t0685300-01(Os03g0685300) Os09t0424200-01(Os09g0424200)
OBR102702165 102712010 102714995
OGL127762879 127768963 127784692
BDI100824869 100831672 100832775
ATS109745571 109750656 109750657 109750658 109781866
TDC119287046 119294673 119299569 119299571 119309613 119309614 119309615 119316287 119325600
TAES123087255 123090702 123095714 123101359 123101360 123114806 123114807 123114808 123120582 123120583 123120584 123120585 123120586 123130467 123133956 123141333
TUA125507075 125507076 125507077 125513826
LPER127293413 127298152 127308077
LRD124651489 124651522 124687988 124689810 124697093 124700490
SBI8055997 8067265 8077619 8079398
ZMA100284196 100284640 103647442 103654868
SITA101760989 101761784 101762195 101775351 101784304
SVS117838578 117841738 117844244 117845678 117864371
PVIR120648314 120653915 120654837 120658852 120672002 120691003 120695695
PHAI112872898 112877917 112881086 112902340
PDA103707253 103709804 103710194 120107775
EGU105034878 105039558 105049884 105053909
MUS103981974 103985479 103989430 103994631 103999020
ZOF121969220 121990468 121994285 122008465 122017323 122027347
DCT110102497 110103081 110113354
PEQ110020638 110023481
AOF109832231 109833835 109842305
MSIN131218713 131229409 131256889
NCOL116260043 116260534 116261033
ATR110007822 18435338 18440851 18440856 18440857
SMOSELMODRAFT_110438 SELMODRAFT_128284
PPP112285930
CSLCOCSUDRAFT_53611 COCSUDRAFT_61836
CVRCHLNCDRAFT_141352
PLJVFPFJ_11004
PFYPFICI_02363 PFICI_15047
PSCOLY89DRAFT_719534
GLZGLAREA_00276
AORAO090011000566
PCSN7525_000317 N7525_007826
NPAUCRNP2_3721
WSEWALSEDRAFT_13494
WICJ056_004110
PFPPFL1_06599
ACANACA1_264000
TETTTHERM_00242040
PTMGSPATT00001723001 GSPATT00009055001 GSPATT00025206001
DSADesal_3256
 » show all
Reference
1  [PMID:21712415]
  Authors
Geu-Flores F, Moldrup ME, Bottcher C, Olsen CE, Scheel D, Halkier BA
  Title
Cytosolic gamma-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis.
  Journal
Plant Cell 23:2456-69 (2011)
DOI:10.1105/tpc.111.083998
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.4.19.16
IUBMB Enzyme Nomenclature: 3.4.19.16
ExPASy - ENZYME nomenclature database: 3.4.19.16
BRENDA, the Enzyme Database: 3.4.19.16
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