KEGG ENZYME: 3.4.21.103

ENZYME: 3.4.21.103

Entry

EC 3.4.21.103               Enzyme

Name

physarolisin;
Dictyostelium discoideum aspartic proteinase;
Dictyostelium discoideum aspartic proteinase E;
Physarum flavicomum aspartic proteinase;
Physarum polycephalum acid proteinase;
Physarum aspartic proteinase;
physaropepsin

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Milk clotting activity. Preferential cleavage of Gly8!Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19!Gly and Phe24!Phe. No action on Ac-Phe-Tyr(I)2.

Comment

Belongs in peptidase family S53. From the slime mold Physarum polycephalum. Is not inhibited by pepstatin, but is blocked by methyl 2-diazoacetamidohexanoate. Closely similar enzymes are found in Dictyostelium discoideum and P. flavicomum. Formerly included in EC 3.4.23.6.

History

EC 3.4.21.103 created 1992 as EC 3.4.23.27 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992), transferred 2003 to EC 3.4.21.103

Orthology

K20756

physarolisin

Genes

DDI:	DDB_G0290333

DPP:	DICPUDRAFT_154796

DFA:

DFA_01300

Reference

Authors

Henney HR, Tavana G.

Title

Purification and some properties of an intracellular acid (carboxyl) proteinase from differentiating haploid cells of Physarum flavicomum.

Journal

Exp Mycol 6:161-170 (1982)

Reference

Authors

Murakami-Murofushi K, Hiratsuka A, Ohta J.

Title

A novel acid protease from haploid amoebae of Physarum polycephalum, and its changes during mating and subsequent differentiation into diploid plasmodia.

Journal

Cell Struct Funct 9:311-315 (1984)

Reference

Authors

North MJ, Whyte A.

Title

Purification and characterization of two acid proteinases from Dictyostelium discoideum.

Journal

J Gen Microbiol 130:123-134 (1984)

Reference

4 [PMID:12673349]

Authors

Wlodawer A, Li M, Gustchina A, Oyama H, Dunn BM, Oda K.

Title

Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases.

Journal

Acta Biochim Pol 50:81-102 (2003)

Reference

5 [PMID:12589815]

Authors

Nishii W, Ueki T, Miyashita R, Kojima M, Kim YT, Sasaki N, Murakami-Murofushi K, Takahashi K

Title

Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase.

Journal

Biochem Biophys Res Commun 301:1023-9 (2003)
DOI:10.1016/S0006-291X(03)00083-4

Sequence

[ag:AAM27198]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.103

IUBMB Enzyme Nomenclature:

3.4.21.103

ExPASy - ENZYME nomenclature database:

3.4.21.103

BRENDA, the Enzyme Database:

3.4.21.103

CAS:	94949-28-7

LinkDB

DBGET integrated database retrieval system