KEGG ENZYME: 3.4.23.21

ENZYME: 3.4.23.21

Entry

EC 3.4.23.21                Enzyme

Name

rhizopuspepsin;
Rhizopus aspartic proteinase;
neurase;
Rhizopus acid protease;
Rhizopus acid proteinase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases

Reaction(IUBMB)

Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave Gln4-His, but does cleave His10!Leu and Val12!Glu in B chain of insulin

Comment

From the zygomycete fungus Rhizopus chinensis. A similar endopeptidase is found in R. niveus [2]. In peptidase family A1 (pepsin A family).

History

EC 3.4.23.21 created 1992 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992)

Reference

Authors

Tsuru D, Hattori A, Tsuji H, Yamamoto T, Fukumoto J.

Title

Studies on mold proteases. Part II. Substrate specificity of acid protease of Rhizopus chinensis.

Journal

Agric Biol Chem 33:1419-1426 (1969)

Reference

Authors

Kurono Y, Chidimatsu M, Horikoshi K, Ikeda Y.

Title

Isolation of a protease from a Rhizopus product.

Journal

Agric Biol Chem 35:1668-1675 (1971)

Reference

3 [PMID:6751894]

Authors

Ohtsuru M, Tang J, Delaney R.

Title

Purification and characterization of rhizopuspepsin isozymes from a liquid culture of Rhizopus chinensis.

Journal

Int J Biochem 14:925-32 (1982)
DOI:10.1016/0020-711x(82)90077-5

Reference

4 [PMID:3313384]

Authors

Suguna K, Padlan EA, Smith CW, Carlson WD, Davies DR.

Title

Binding of a reduced peptide inhibitor to the aspartic proteinase from Rhizopus chinensis: implications for a mechanism of action.

Journal

Proc Natl Acad Sci U S A 84:7009-13 (1987)
DOI:10.1073/pnas.84.20.7009

Other DBs

ExplorEnz - The Enzyme Database:

3.4.23.21

IUBMB Enzyme Nomenclature:

3.4.23.21

ExPASy - ENZYME nomenclature database:

3.4.23.21

BRENDA, the Enzyme Database:

3.4.23.21

CAS:

9074-09-3

LinkDB

DBGET integrated database retrieval system