KEGG   ENZYME: 3.4.24.33
Entry
EC 3.4.24.33                Enzyme                                 
Name
peptidyl-Asp metalloendopeptidase;
endoproteinase Asp-N;
peptidyl-Asp metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Cleavage of Xaa!Asp, Xaa!Glu and Xaa!cysteic acid bonds
Comment
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.
History
EC 3.4.24.33 created 1992
Orthology
K23743  peptidyl-Asp metalloendopeptidase
Genes
RBAD: H2866_19375
PAO: Pat9b_1661
XBV: XBW1_0130
XCC: XCC0955
XCB: XC_3280
XCA: xcc-b100_3396
XCP: XCR_1151
XCV: XCV1064 XCV3406
XAX: XACM_1015
XAC: XAC1034
XCI: XCAW_03548
XCT: J151_01160
XCJ: J158_01157
XCU: J159_01157
XCN: J169_01156
XCW: J162_01156
XCR: J163_01156
XCM: J164_01156
XCF: J172_01151
XFU: XFF4834R_pla00230
XAO: XAC29_05235
XSA: SB85_07835 SB85_08055
XTN: FD63_05115
XFR: BER92_13795 BER92_15345
XVE: BJD12_01905 BJD12_18125
XPE: BJD13_11595 BJD13_23105
XHR: XJ27_00860 XJ27_10265
XGA: BI317_05960 BI317_16060
XPH: XppCFBP6546_22875(XppCFBP6546P_22875)
XVA: C7V42_05585 C7V42_14540
XAN: AC801_22495
XAR: XB05_21995
XCZ: EBN15_04075
XEU: XSP_001016
XHD: LMG31886_33120
XPR: MUG10_04890
XAG: HEP73_03312
XDY: NYR95_05860
XAS: HEP74_03126
XHA: PG878_04630
XRY: QN244_16380
SML: Smlt0970
SMT: Smal_0815
BUJ: BurJV3_0821
SMZ: SMD_0844
SRH: BAY15_0985
STEN: CCR98_04395
STEM: CLM74_04360
STES: MG068_04075
SINC: DAIF1_08730
SPAQ: STNY_R08790
SGEN: RKE57_03950
SOU: PDM29_11780
PMEX: H4W19_16485
LAB: LA76x_0516 LA76x_1092 LA76x_1409 LA76x_3981(lieA)
LAQ: GLA29479_2060 GLA29479_3573(lieA) GLA29479_4589
LCP: LC55x_0421 LC55x_1143 LC55x_4009(lieA) LC55x_4359
LGU: LG3211_1059 LG3211_2566 LG3211_3821(lieA)
LEZ: GLE_0242 GLE_1028 GLE_1441 GLE_3845
LEM: LEN_1306 LEN_3884 LEN_4700
LMB: C9I47_2626
LSX: H8B22_09870
LAUX: LA521A_09120
LYA: RDV84_01510 RDV84_05400 RDV84_09105 RDV84_13720 RDV84_24925
LSF: I8J32_001230 I8J32_008260 I8J32_014540
LUG: FPZ22_04995
TCN: H9L16_07690
DYG: DYGSA30_00460
DKO: I596_1765 I596_604 I596_650
XBA: C7S18_01130
TAMN: N4264_06440 N4264_10800 N4264_11970 N4264_20215
PPF: Pput_0320
PPG: PputGB1_2257 PputGB1_4897
PPW: PputW619_4908
PPB: PPUBIRD1_0329
PPI: YSA_05605
PPUN: PP4_03260
PSEC: CCOS191_0333
PMAM: KSS90_01620
PBEJ: PSH84_15565
PSKP: KU43P_31400
KAK: Kalk_08295
PSPI: PS2015_1896
MBAH: HYN46_06595 HYN46_06600 HYN46_14700
SWD: Swoo_3429
SBK: SHEWBE_3871
COV: EKO29_13625
THAT: H3N35_11850
TACT: SG35_012465 SG35_019475
TVD: SG34_016995
PSM: PSM_B0114
PIA: PI2015_3157
PPHE: PP2015_3913
PBW: D172_016615 D172_020850
PRR: AT705_23820
PLZ: S4054249_12625
PALN: B0W48_02315 B0W48_03170 B0W48_07280
PPIS: B1L02_21050
PEA: PESP_b0216
PSPO: PSPO_a1218
PART: PARC_b0067
PTD: PTET_b0146
PSEN: PNC201_19910
PAGA: PAGA_b0173
PCAR: PC2016_3768
PVB: J5X90_22990
PDV: FFU37_17645
PFLI: CTT31_21730
PMAZ: R5H13_21780
PSMM: PspMM1_32470
AMAC: MASE_15605
AMG: AMEC673_15900
AMK: AMBLS11_15350
PAEW: KIH87_15410
CJA: CJA_0276(cbp6B) CJA_1294
CEB: B0D95_08930 B0D95_08940
CELL: CBR65_21775 CBR65_21900
CEG: D0C16_00635 D0C16_09470
SDE: Sde_3927(cbm6F)
MII: BTJ40_08990 BTJ40_11780
MICT: FIU95_12240 FIU95_12255
MHYD: GTQ55_02330
MAH: MEALZ_0963
MBUR: EQU24_15580
MMAI: sS8_5288
MSZE: MSZNOR_2114 MSZNOR_2155
MECH: Q9L42_008585
TDU: QJT80_02315
PLEI: Q9312_16675
AHA: AHA_0616
AHY: AHML_03155
AHD: AI20_16255
AHR: V428_03310
AHP: V429_03310
AHJ: V469_20010
AHH: RY45_03520
AHI: VU14_19400
AAJ: BOQ57_02995
ASR: WL1483_1999
ADH: CK627_06890
AEJ: E5E97_07360
CARI: FNU76_03255
ETB: N7L95_02805 N7L95_21425
MEE: DA075_06885
CMET: K6K41_08865 K6K41_08870
PLEO: OHA_1_03599
SAND: H3309_16830
DGG: DGI_1524
BDQ: CIK05_14290
SFI: SFUL_513
SVE: SVEN_7265
SCI: B446_00585 B446_34705
SVU: B1H20_01525
SALF: SMD44_08083
SFK: KY5_4622
SFIC: EIZ62_30920
SSPB: CP982_36325
SFP: QUY26_02875
KAB: B7C62_01350
NSN: EXE58_09945
NOQ: LN652_11260
LEPT: JVX88_05330
PBF: CFX0092_A2517
GFM: Enr17x_36110
KST: KSMBR1_3925
UAM: UABAM_03214
PBAP: Pla133_37950
ABAC: LuPra_04139 LuPra_04140
LUB: TBR22_A17660 TBR22_A30410
PSPC: Strain318_000293
NKO: Niako_0622
DFE: Dfer_2081
DSN: HWI92_18180
IRO: RT717_01015
CAGG: HYG79_15140
CIH: ATE47_12820
CHRY: CEY12_11165
CPIP: CJF12_17550
CHRS: EAG08_07920
CTAI: NCTC12078_02720
CORZ: MTP08_01210
CFLU: ODZ84_11135
CJG: NCTC13459_02463
RMR: Rmar_0099 Rmar_2053
RMG: Rhom172_0097
NMV: NITMOv2_4112
NKF: Nkreftii_003441
NLI: G3M70_00595
NVA: G3M78_04705
 » show all
Reference
1  [PMID:236771]
  Authors
Porzio MA, Pearson AM.
  Title
Isolation of an extracellular neutral proteinase from Pseudomonas fragi.
  Journal
Biochim Biophys Acta 384:235-41 (1975)
DOI:10.1016/0005-2744(75)90112-6
Reference
2  [PMID:7188696]
  Authors
Drapeau GR.
  Title
Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi.
  Journal
J Biol Chem 255:839-40 (1980)
Reference
3  [PMID:2669754]
  Authors
Ingrosso D, Fowler AV, Bleibaum J, Clarke S
  Title
Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins.
  Journal
Biochem Biophys Res Commun 162:1528-34 (1989)
DOI:10.1016/0006-291x(89)90848-6
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.33
IUBMB Enzyme Nomenclature: 3.4.24.33
ExPASy - ENZYME nomenclature database: 3.4.24.33
BRENDA, the Enzyme Database: 3.4.24.33
CAS: 55576-49-3
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