KEGG   ENZYME: 3.5.1.138
Entry
EC 3.5.1.138                Enzyme                                 
Name
lipoamidase;
pyruvate dehydrogenase inactivase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
[lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine amidohydrolase
Reaction(IUBMB)
a [lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine + H2O = a [lipoyl-carrier protein]-L-lysine + (R)-lipoate
Substrate
[lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine;
H2O [CPD:C00001]
Product
[lipoyl-carrier protein]-L-lysine [CPD:C16240];
(R)-lipoate [CPD:C16241]
Comment
The enzyme, characterized from the bacterium Enterococcus faecalis, is a member of the Ser-Ser-Lys triad amidohydrolase family. cf. EC 2.3.1.313, NAD-dependent lipoamidase.
History
EC 3.5.1.138 created 2023
Orthology
K26817  lipoamidase
Genes
LNA: RIN67_08995
EFA: EF1033
EFL: EF62_1466
EFI: OG1RF_10766
EFD: EFD32_0845
EFS: EFS1_0861
EFN: DENG_01166
EFQ: DR75_92
ENE: ENT_30580
HOV: EQF90_004370
Reference
1  [PMID:13549405]
  Authors
REED LJ, KOIKE M, LEVITCH ME, LEACH FR.
  Title
Studies on the nature and reactions of protein-bound lipoic acid.
  Journal
J Biol Chem 232:143-58 (1958)
Reference
2  [PMID:14086741]
  Authors
SUZUKI K, REED LJ.
  Title
LIPOAMIDASE.
  Journal
J Biol Chem 238:4021-5 (1963)
Reference
3  [PMID:15528186]
  Authors
Jiang Y, Cronan JE.
  Title
Expression cloning and demonstration of Enterococcus faecalis lipoamidase (pyruvate dehydrogenase inactivase) as a Ser-Ser-Lys triad amidohydrolase.
  Journal
J Biol Chem 280:2244-56 (2005)
DOI:10.1074/jbc.M408612200
  Sequence
[efs:EFS1_0861]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.138
IUBMB Enzyme Nomenclature: 3.5.1.138
ExPASy - ENZYME nomenclature database: 3.5.1.138
BRENDA, the Enzyme Database: 3.5.1.138
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