KEGG ENZYME: 3.5.1.77

ENZYME: 3.5.1.77

Entry

EC 3.5.1.77                 Enzyme

Name

N-carbamoyl-D-amino-acid hydrolase;
D-N-carbamoylase;
N-carbamoylase (ambiguous);
N-carbamoyl-D-amino acid hydrolase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

N-carbamoyl-D-amino-acid amidohydrolase

Reaction(IUBMB)

an N-carbamoyl-D-amino acid + H2O = a D-amino acid + NH3 + CO2 [RN:R02190]

Reaction(KEGG)

R02190

Substrate

N-carbamoyl-D-amino acid [CPD:C05226];
H2O [CPD:C00001]

Product

D-amino acid [CPD:C00405];
NH3 [CPD:C00014];
CO2 [CPD:C00011]

Comment

This enzyme, along with EC 3.5.1.87 (N-carbamoyl-L-amino-acid hydrolase), EC 5.1.99.5 (hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the "hydantoinase process", which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids [2]. It has strict stereospecificity for N-carbamoyl-D-amino acids and does not act upon the corresponding L-amino acids or on the N-formyl amino acids, N-carbamoyl-sarcosine, -citrulline, -allantoin and -ureidopropanoate, which are substrates for other amidohydrolases.

History

EC 3.5.1.77 created 1999, modified 2008

Orthology

K01459

N-carbamoyl-D-amino-acid hydrolase

Genes

SYM:	K6K13_14705

GQU:	AWC35_02065 AWC35_21320

BGJ:	AWC36_06180 AWC36_10120

BRB:	EH207_07550 EH207_15550

BNG:	EH206_14055 EH206_22905

BIZ:	HC231_12695

LBQ:	CKQ53_07460 CKQ53_16475

LPOP:

I6N93_10735 I6N93_14635

PAO:	Pat9b_4333

PPAO:

K3169_19460

AFY:	BW247_12330

HAA:	A5892_06310

MGEO:

CFI10_17390

RPJ:	N234_35240

CNC:	CNE_BB1p08270

REU:	Reut_B4535

CCUP:

BKK81_02680

CUP:	BKK80_02025

CUU:	BKK79_10250

CPAU:

EHF44_27650

COX:	E0W60_29015 E0W60_32345

CUK:	KB879_35925

CUPP:

CTP10_R41150

PPK:	U875_08630 U875_19865

PPNO:

DA70_01355 DA70_12555

PPNM:

LV28_04990 LV28_18140

PRB:	X636_00975 X636_14310

PSPU:

NA29_16365

PAPI:

SG18_12590

PVE:	UC34_02240

PFG:	AB870_13445

PFIB:

PI93_003720

BUO:	BRPE64_CCDS07700

CABK:

NK8_66600

BPT:

Bpet2330

BAV:

BAV0674

BTRM:

SAMEA390648702057

BBRO:

BAU06_21120

BFZ:	BAU07_05280

BOH:	AKI39_20360

BGM:	CAL15_06705

BOJ:	CBF45_15540

BGV:	CAL12_20635 CAL12_23115

AXY:	AXYL_00271(dcaS1) AXYL_03527(dcaS2)

AXO:	NH44784_019281

AXN:	AX27061_0269

AXX:	ERS451415_00269

ADT:	APT56_20675

AIS:	BUW96_07545 BUW96_27085

ASW:	CVS48_25380

ACHB:

DVB37_19280

ADY:	HLG70_00820 HLG70_05395 HLG70_20395

ACHO:

H4P35_01440 H4P35_05255 H4P35_08235 H4P35_18995

AMUI:

PE062_02275 PE062_05010 PE062_15725 PE062_25405

APES:

FOC84_11505 FOC84_27825

AAEG:

RA224_01295 RA224_15550

ASEL:

RAS12_20255

ACHM:

V6V88_01415 V6V88_05580 V6V88_08335

ACHA:

KYT87_19950

AVH:	WHX56_06215

ACHH:

ABFG95_06415

AFA:	UZ73_18230

AFQ:

AFA_08930

AAQU:

D3M96_10170

ALCN:

MID00_18480

PHN:	PAEH1_03445

BOZ:	DBV39_09420 DBV39_17550

ODH:	DHf2319_02080

PACR:

FXN63_03955 FXN63_08570

KGY:	EHF36_15560

RFR:	Rfer_0149 Rfer_0157

RLH:	MIZ03_1522

POL:

Bpro_1497

PNA:

Pnap_4069

ASAH:

AVXHC19_11700

AAA:

Acav_1139

AMON:

H9L24_16385

CKE:	B5M06_03605

CAQT:

KAQ61_08385 KAQ61_13975

CODO:

LAD35_11860

CTEZ:

CT3_35760

AANT:

HUK68_01705

CENP:

M9799_15170

COMB:

MJ205_05410 MJ205_08415

CSQU:

N4T19_04310 N4T19_07160

RTA:

Rta_13990

HRB:	Hrubri_1289

HFR:	G5S34_07720

GLC:	JQN73_16720

XYK:	GT347_05560 GT347_11180 GT347_14065

XYG:	R9X41_22385

AZR:	CJ010_02050

ACOM:

CEW83_16295

MLN:	A9174_30240

MAMO:

A6B35_10445

MESM:

EJ066_08015

MHUA:

MCHK_0583

MJR:	EB229_30840

MERD:

EB233_27045

MOH:	IHQ72_02920

MESJ:

MJ8_39320

MESO:

BSQ44_08220

RPOD:

E0E05_03520

KMN:	HW532_13320

AVQ:	HRR99_15935

AVV:	RvVAT039_pl03420

AVI:

Avi_7123

ROY:	G3A56_25620

NEN:	NCHU2750_43480

NPM:	QEO92_30500

BJA:	bll7207(bll7207) blr2576(blr2576) blr3330(blr3330)

BJU:	BJ6T_21980 BJ6T_64620

BJP:	RN69_10750 RN69_31320

BRA:	BRADO1782 BRADO1790 BRADO3018

BBT:	BBta_2095 BBta_2106 BBta_5123

BRS:	S23_11230 S23_47040 S23_54090

AOL:	S58_45840 S58_57530 S58_57650

BRAD:

BF49_1415 BF49_2210 BF49_4325

BRO:	BRAD285_2771 BRAD285_5501 BRAD285_5513

BOT:	CIT37_19090 CIT37_37125 CIT37_40810

BRQ:	CIT40_07380 CIT40_10890 CIT40_27350

BGQ:	X265_07140 X265_24710 X265_28160

BGZ:	XH91_06600 XH91_22750 XH91_26140

BSYM:

CIT39_06615 CIT39_09900 CIT39_26325

BBET:

F8237_06985

BARH:

WN72_17995 WN72_19615 WN72_19655

BVZ:	BRAD3257_5411 BRAD3257_6466 BRAD3257_8085

BDG:	LPJ38_19675

BBAN:

J4G43_009285 J4G43_018970

BCAN:

BcanWSM471_11980 BcanWSM471_15640 BcanWSM471_31470

BBRA:

QA636_11695 QA636_11700

BCOU:

IC761_05805 IC761_22955 IC761_26410

BXN:	I3J27_12915 I3J27_31995

BROS:

QUH67_13280 QUH67_13365

BRAZ:

LRP30_10815 LRP30_15400 LRP30_15440

BOY:	LQG66_09465 LQG66_15115 LQG66_15190

BRAY:

ML401_08160

RBK:	E0H22_22820

RHOR:

ONR75_04355 ONR75_10680

VGO:	GJW-30_1_04016

TRB:	HB776_00680

TARD:

RSO67_25120

BVV:	BHK69_12205 BHK69_25495

BVES:

QO058_20090 QO058_24750

BOP:	AXW83_01395 AXW83_10640

BOS:	BSY19_1752 BSY19_3589

BOI:	BLM15_13055

BOF:	FQV39_11400 FQV39_21360

BOW:	NWE53_00320 NWE53_17080

BOSE:

RMR04_08630 RMR04_21880

AZC:	AZC_2017 AZC_2488 AZC_2732

MAQU:

Maq22A_c06080

MEE:	DA075_21910

MTEA:

DK419_15100 DK419_15245

MIND:

mvi_46910 mvi_55840

DEI:	C4375_16580

DNP:	N8A98_21145

DRI:	QOV41_03310 QOV41_03360

PLEO:

OHA_1_03075

HDI:

HDIA_3985

MEY:	TM49_22335

MAAD:

AZF01_19145

MMED:

Mame_04417

MLUT:

JET14_20020

BRN:	D1F64_21940

PHR:	C6569_06625 C6569_08555 C6569_21545

PSTG:

E8M01_05625 E8M01_16700 E8M01_26435 E8M01_32015

PAQT:

E8L99_00285 E8L99_09540 E8L99_15630

LIZ:	LGH83_14250

ACUT:

MRB58_00385

MFLG:

ABS361_09275

KVL:	KVU_0825 KVU_PB0054

KVU:	EIO_1330 EIO_3237

CON:	TQ29_07940

YPAC:

CEW88_01065 CEW88_23890

YAN:	AYJ57_10415 AYJ57_23835

SULZ:

C1J03_23710

AHT:	ANTHELSMS3_01544(amiF)

SAGU:

CDO87_02775

SSTL:

ABFK29_15970

THAA:

CFI11_03685 CFI11_15105

LSAL:

KBK07_10415

RSP:

RSP_3461

RSH:	Rsph17029_3106

RSQ:	Rsph17025_2066

RSK:	RSKD131_3627

CAZT:

LV780_09225

PYE:	A6J80_20965

PSAP:

JHX88_05470

PFIS:

JHX87_03095

RHC:

RGUI_1328

THW:	BMG03_04910

TEC:	AKL02_004640

GEH:	HYN69_10460

PPRU:

FDP22_04880 FDP22_18770

PSHQ:

F3W81_03065 F3W81_06000

HDH:	G5B40_02335 G5B40_15630

CMAG:

CBW24_01705 CBW24_04755

AMAQ:

GO499_03875

MANH:

LA6_000254 LA6_000868 LA6_001826 LA6_002930 LA6_003143

NOG:	GKE62_14875

GAL:	A0U94_09480

GSP:	IGS75_03230

GXY:

GLX_01010

ATO:	CIW82_00100 CIW82_00705

NCH:	A0U93_11475

LCK:	HN018_03445

KHA:	IFJ82_08940

RAP:	RHOA_3660 RHOA_5130

NGU:	QN315_12765

RGI:	RGI145_06175 RGI145_18315 RGI145_19745

ROS:	CTJ15_11225

RMUC:

FOB66_02190 FOB66_07990

RMT:	IAI58_08255

RFL:	Rmf_30010 Rmf_30050

RCV:	PFY06_08160 PFY06_13065

SRF:	LHU95_06960 LHU95_14475

ELIO:

KO353_11735 KO353_11915

SHUM:

STHU_24680 STHU_48090

SVC:	STVA_30970 STVA_48280

STEL:

STAQ_22290 STAQ_43420

FER:	FNB15_10120

AALU:

ABWI00_13995

HTQ:	FRZ44_28060

HADH:

FRZ61_19520

NACI:

NUH88_16540 NUH88_17850 NUH88_20375

THAR:

T8K17_13570 T8K17_21805

GAZ:	Pan241w_18590

GAW:	V144x_40270

GSD:	M3M28_11550

ACRY:

AC20117_08900

SERW:

FY030_06590

BLUT:

EW640_08835

AYM:	YM304_01590

IAM:	HC251_12255

IMA:	PO878_02825

LEPV:

J4558_15405 J4558_27205

» show all

Reference

1 [PMID:8462543]

Authors

Ogawa J, Shimizu S, Yamada H.

Title

N-carbamoyl-D-amino acid amidohydrolase from Comamonas sp. E222c purification and characterization.

Journal

Eur J Biochem 212:685-91 (1993)
DOI:10.1111/j.1432-1033.1993.tb17706.x

Reference

2 [PMID:11849938]

Authors

Altenbuchner J, Siemann-Herzberg M, Syldatk C

Title

Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids.

Journal

Curr Opin Biotechnol 12:559-63 (2001)
DOI:10.1016/S0958-1669(01)00263-4

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.77

IUBMB Enzyme Nomenclature:

3.5.1.77

ExPASy - ENZYME nomenclature database:

3.5.1.77

BRENDA, the Enzyme Database:

3.5.1.77

CAS:	71768-08-6

LinkDB

DBGET integrated database retrieval system