KEGG   ENZYME: 3.5.1.94
Entry
EC 3.5.1.94                 Enzyme                                 
Name
gamma-glutamyl-gamma-aminobutyrate hydrolase;
gamma-glutamyl-GABA hydrolase;
PuuD;
YcjL;
4-(gamma-glutamylamino)butanoate amidohydrolase;
4-(L-gamma-glutamylamino)butanoate amidohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
4-(gamma-L-glutamylamino)butanoate amidohydrolase
Reaction(IUBMB)
4-(gamma-L-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate [RN:R07419]
Reaction(KEGG)
R07419
Substrate
4-(gamma-L-glutamylamino)butanoate [CPD:C15767];
H2O [CPD:C00001]
Product
4-aminobutanoate [CPD:C00334];
L-glutamate [CPD:C00025]
Comment
Forms part of a putrescine-utilizing pathway in Escherichia coli, in which it has been hypothesized that putrescine is first glutamylated to form gamma-glutamylputrescine, which is oxidized to 4-(gamma-glutamylamino)butanal and then to 4-(gamma-glutamylamino)butanoate. The enzyme can also catalyse the reactions of EC 3.5.1.35 (D-glutaminase) and EC 3.5.1.65 (theanine hydrolase).
History
EC 3.5.1.94 created 2006, modified 2011
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K09473  gamma-glutamyl-gamma-aminobutyrate hydrolase
Genes
ECOb1298(puuD)
ECJJW1291(puuD)
ECDECDH10B_1415(puuD)
EBWBWG_1130(puuD)
ECOKECMDS42_1096(puuD)
ECEZ2490(ycjL)
ECSECs_1875(puuD)
ECFECH74115_1940(puuD)
ETWECSP_1823(puuD)
ELXCDCO157_1795(puuD)
EOIECO111_1681(puuD)
EOJECO26_1865(puuD)
EOHECO103_1462(puuD)
ECOOECRM13514_1724(puuD)
ECOHECRM13516_1689(puuD)
ESLO3K_13840(puuD)
ESOO3O_11790(puuD)
ESMO3M_13815(puuD)
ECKEC55989_1460(puuD)
EOKG2583_1645(puuD)
ELRECO55CA74_08010(puuD)
ELHETEC_1402
ECWEcE24377A_1508(puuD)
EUNUMNK88_1634
ECOAAPECO78_10365(puuD)
ECXEcHS_A1413(puuD)
ECMEcSMS35_1824(puuD)
ECYECSE_1350
ECRECIAI1_1323(puuD)
EUMECUMN_1604(puuD)
ECTECIAI39_1649(puuD)
EOCCE10_1546(puuD)
EBRECB_01275(ycjL)
EBLECD_01275(puuD)
EBEB21_01286(puuD)
EBDECBD_2319
ELOEC042_1426(puuD)
ECLEcolC_2327
EKOEKO11_2552
EKFKO11_16320(puuB)
EDHEcDH1_2348
EDJECDH1ME8569_1240(puuD)
ELWECW_m1394(puuB)
ELLWFL_06795(puuB)
ELPP12B_c1796(puuD)
ECOLLY180_06610(puuD)
EALEAKF1_ch0120c(puuD)
ESZFEM44_21590(puuD)
SFLSF1303(puuD)
SFXS1385(ycjL)
SFVSFV_1312(ycjL)
SFESFxv_1476(puuD)
SFNSFy_1872
SFSSFyv_1930
SFTNCTC1_01378(puuD)
SSNSSON_1842(ycjL)
SDZAsd1617_02621
SHQA0259_10615(puuD)
ENCECL_02226 ECL_02227
ENLA3UG_10015(puuD)
ECLGEC036_19400
ECLNECNIH4_12705
EAUDI57_08925
EKBBFV64_10365
ENOECENHK_09940(puuD)
ECANCWI88_14360
ERNBFV67_09510
ECHGFY206_11405(puuD)
EBGFAI37_19215(puuD)
ENDA4308_14655
ENZG0034_09780(puuD)
ENBELK40_10330
ESAESA_01202 ESA_02079
CSKES15_1440 ES15_2236(puuD)
CSZCSSP291_05720 CSSP291_09885(puuD)
CSJCSK29544_02519 CSK29544_03372
CCONAFK62_12455
CDMAFK67_08760 AFK67_13070
CSIP262_02096 P262_03230
CMJAFK66_007010 AFK66_010920
CUIAFK65_08620 AFK65_12765
CMWAFK63_05570 AFK63_09870
CTUCTU_18850(puuD) CTU_27130(puuD)
KPNKPN_01020
KPUKP1_2005
KPMKPHS_18950
KPPA79E_3213
KPHKPNIH24_19035
KPZKPNIH27_08950
KPVKPNIH29_09480
KPWKPNIH30_09995
KPYKPNIH31_09370
KPGKPNIH32_09835
KPCKPNIH10_09490
KPQKPR0928_09660
KPTVK055_1457
KPOKPN2242_08160(puuD)
KPRKPR_3502(puuD)
KPJN559_3267
KPID364_05305(puuD)
KPAKPNJ1_03508
KPSKPNJ2_03497
KPXPMK1_03360(puuD)
KPBFH42_00955
KPNEKU54_016665
KPNULI86_16975
KPNKBN49_2108(puuD)
KVAKvar_3359
KPEKPK_3540(puuD)
KPKA593_19600
KVDKR75_17270
KVQSP68_23650
KOXKOX_16930(puuD)
KOEA225_2234
KOYJ415_20605(puuD)
KOMHR38_15450
KMIVW41_14390
KOKKONIH1_10930
KOCAB185_25205
KQUAVR78_13930
EAEEAE_15930(puuD)
EARCCG30721
KQVB8P98_18170
KLLBJF97_11205
KLWDA718_17645
KARLGL98_16125(puuD)
KGRJJJ10_17595(puuD)
RORRORB6_09560(puuD)
RONTE10_11020
RPLNB1209_17455
RAODSD31_16195
RTGNCTC13098_04767(puuD)
CROROD_03571(puuD)
CFDCFNIH1_11620
CBRAA6J81_11670
CYOCD187_18790
CPOTFOB25_06245(puuD)
CFQC2U38_05160
CIEAN232_24815
CITZE4Z61_12075(puuD)
CARSE1B03_06250(puuD)
CNTJT31_20730
CEMLH23_20345
CENLH86_18980
PGELG71_13295
ESCEntcl_1709
KLEAO703_13445
LAZA8A57_07990
YREHEC60_09605(puuD)
SGOEA8O29_008300(puuD)
IZHFEM41_12210(puuD)
PSGCG163CM_17500(puuD)
EBFD782_2780
EBUCUC76_06185
PSTSE05_49390
SMARSM39_1530(puuD)
SMACSMDB11_1340(puuD)
SMWSMWW4_v1c20860(puuD)
SPESpro_2067
SRRSerAS9_2004
SRLSOD_c18790(puuD)
SRYM621_10365(puuD)
SPLYQ5A_010395(puuD)
SRSSerAS12_2004
SRASerAS13_2005
SMAFD781_1891
SLQM495_10020(puuD)
SERFL085_18335(puuD)
SERSSERRSCBI_09775(puuD)
SFWWN53_19615
SFGAV650_14990
SRZAXX16_3238
SERMCLM71_09390
SQUE4343_05880(puuD)
SFJSAMEA4384070_2124(puuD)
SOFNCTC11214_04759(puuD)
SSURATE40_006925
SURIJ0X03_13570(puuD)
SRHZFO014_00160(puuD)
SENPKHA73_10455(puuD)
SFOZ042_23795
RAHRahaq_1577
RACEJHW33_02135(puuD)
RAQRahaq2_1641
RAAQ7S_07625(puuD)
ROXBV494_03020
RVCJ9880_15625(puuD)
EAMEGXP68_07185(puuD)
RBADH2866_00980(puuD)
EBIEbC_29020(puuD)
EGEEM595_2350(puuD)
EPECI789_04200
ERWIGN242_07300(puuD)
ERPLJN55_15060(puuD)
PAMPANA_2463(puuD)
PLFPANA5342_1623(ycjL)
PAJPAJ_1760(puuD)
PAQPAGR_g1566
PVAPvag_1946(ycjL)
PAGGAL522_16625
PAOPat9b_2555
KLNLH22_09815
PANTPSNIH1_15800
PANPPSNIH2_05245
PAGCBEE12_00320
PSTWDSJ_15840
PALHB1H58_14670
PANSFCN45_12315(puuD)
PEYEE896_06980(puuD)
PDISD8B20_10935
PERLAC65_09785(puuD)
PGZC2E15_13345
PCDC2E16_13555
MINTC7M51_01857(puuD)
MTHIC7M52_00953(puuD)
HAVAT03_07545
HPARAL518_18045
OPODSM2777_12830
VFUvfu_A02175
VFLAL536_14610
PSOPSYCG_04620
PSPGAK823_04435
PSYGAK825_04405
LCJNCTC11976_00395(pyrG_1)
ZPLZBT109_0404
HAAA5892_03645
OCEGU3_14520(puuD)
ZDFAN401_15155
DNODNO_0653
 » show all
Reference
1  [PMID:15590624]
  Authors
Kurihara S, Oda S, Kato K, Kim HG, Koyanagi T, Kumagai H, Suzuki H.
  Title
A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12.
  Journal
J Biol Chem 280:4602-8 (2005)
DOI:10.1074/jbc.M411114200
  Sequence
[eco:b1298]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.94
IUBMB Enzyme Nomenclature: 3.5.1.94
ExPASy - ENZYME nomenclature database: 3.5.1.94
BRENDA, the Enzyme Database: 3.5.1.94
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