KEGG   ENZYME: 3.5.2.1
Entry
EC 3.5.2.1                  Enzyme                                 
Name
barbiturase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
Sysname
barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming)
Reaction(IUBMB)
barbiturate + H2O = 3-oxo-3-ureidopropanoate [RN:R02139]
Reaction(KEGG)
R02139;
(other) R03870 R10777
Substrate
barbiturate [CPD:C00813];
H2O [CPD:C00001]
Product
3-oxo-3-ureidopropanoate [CPD:C15607]
Comment
Contains zinc and is specific for barbiturate as substrate [3]. Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 1.17.99.4 (uracil/thymine dehydrogenase) and EC 3.5.1.95 (N-malonylurea hydrolase). It was previously thought that the end-products of the reaction were malonate and urea but this has since been disproved [2]. May be involved in the regulation of pyrimidine metabolism, along with EC 2.4.2.9, uracil phosphoribosyltransferase.
History
EC 3.5.2.1 created 1961, modified 2006
Pathway
ec00240  Pyrimidine metabolism
ec01100  Metabolic pathways
Orthology
K19794  barbiturase
Genes
PSTGE8M01_13880
RHARHA1_ro05296
RERRER_58820(bar)
REYO5Y_28310
REBXU06_28640
RQIC1M55_30125
ROPROP_27990(bar)
ROAPd630_LPD01826
RFAA3L23_03491(bar)
RHSA3Q41_04764(bar)
RHODAOT96_09775
RKOJWS14_16515
ICAIntca_1068
PEIH9L10_01950
SERWFY030_02810
ORNDV701_12635
BRIFDF13_14170
BLUTEW640_05135
MIKFOE78_19255
NCANoca_1505 Noca_4270
NDKI601_1320(bar)
NOYEXE57_06140
NANOG5V58_15295
NMESH9L09_19375
NROK8W59_11195
NOQLN652_19335
NCQK6T13_05240
NPCKUV85_11075
NPSKRR39_11145
PSIMKR76_07390
MUZH4N58_05180
STRREKD16_19160(bar)
SAIUJ4H86_08125
AGRAAGRA3207_007139
SROSros_6541
NOABKM31_01725
NOWGBF35_36215
NCXNocox_29475(bar1)
NGNLCN96_40605
TBITbis_2213
NMLNamu_5328
GOBGobs_0181
BSDBLASA_0154(bar) BLASA_4559
MMARMODMU_0176(bar)
SVISvir_20970
SACCEYD13_13280(bar2)
PSEHXF36_23335
PAUTPdca_02970
CAICaci_1879
EKEEK0264_00860
JTLM6D93_08835
ERZER308_05780
 » show all
Reference
1  [PMID:12981104]
  Authors
HAYAISHI O, KORNBERG A.
  Title
Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes.
  Journal
J Biol Chem 197:717-32 (1952)
Reference
2  [PMID:11485332]
  Authors
Soong CL, Ogawa J, Shimizu S.
  Title
Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase.
  Journal
Biochem Biophys Res Commun 286:222-6 (2001)
DOI:10.1006/bbrc.2001.5356
  Sequence
Reference
3  [PMID:11748240]
  Authors
Soong CL, Ogawa J, Sakuradani E, Shimizu S
  Title
Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism.
  Journal
J Biol Chem 277:7051-8 (2002)
DOI:10.1074/jbc.M110784200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.2.1
IUBMB Enzyme Nomenclature: 3.5.2.1
ExPASy - ENZYME nomenclature database: 3.5.2.1
BRENDA, the Enzyme Database: 3.5.2.1
CAS: 9025-16-5
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