Entry
Name
nitrilase;
acetonitrilase;
benzonitrilase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles
BRITE hierarchy
Sysname
nitrile aminohydrolase
Reaction(IUBMB)
a nitrile + 2 H2O = a carboxylate + NH3 [RN:
R00540 ]
Reaction(KEGG)
Substrate
Product
Comment
Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC
4.2.1.84 nitrile hydratase.
History
EC 3.5.5.1 created 1965, modified 1989
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
Genes
CEL : CELE_ZK1058.6(nit-1)
CBR : CBG_03597(Cbr-nit-1)
ATH : AT3G44300(NIT2) AT3G44310(NIT1) AT3G44320(NIT3)
ALY : 110228405 9311726 9313400
CRB : 17877697 17886221 17886780
CSAT : 104710891 104710892 104766496 104773049 104780280 104780283 104790687 104790689
EUS : EUTSA_v10010499mg EUTSA_v10010504mg EUTSA_v10023557mg
BRP : 103848073 103851084(NIT-T1) 103856931 103865089
BNA : 106347471 106348366 106354428 106387438 106402388 106403172 106437374
BOE : 106320293 106327314 106333257 106339483
RSZ : 108813168 108846529 130505847
PIC : PICST_36697(NIT1) PICST_50366(NRL2)
PGU : PGUG_04844 PGUG_04845
NCR : NCU00437 NCU01838 NCU05387
NTE : NEUTE1DRAFT117501(NEUTE1DRAFT_117501) NEUTE1DRAFT82398(NEUTE1DRAFT_82398)
SMP : 10804094(SMAC4_04139) 10806312(SMAC4_01874)
PAN : PODANSg1416 PODANSg6347 PODANSg6829
PBEL : QC761_305180 QC761_309820
PPSD : QC762_0027220 QC762_305180 QC762_309820
PPSP : QC763_305180 QC763_309820
PPSA : QC764_305180 QC764_309820
TMN : UCRPA7_1468 UCRPA7_1476
FGR : FGSG_00051 FGSG_01698 FGSG_06480 FGSG_11357
FPU : FPSE_07830 FPSE_10135 FPSE_11461
FPOA : FPOAC1_000035 FPOAC1_001816 FPOAC1_004270 FPOAC1_010176
FVN : FVRRES_00096 FVRRES_02030 FVRRES_04371 FVRRES_04586 FVRRES_04765 FVRRES_08378 FVRRES_11156
FVR : FVEG_00065 FVEG_01752 FVEG_01796 FVEG_06618 FVEG_09776 FVEG_11864 FVEG_11942 FVEG_13327 FVEG_17125
FOX : FOXG_02875 FOXG_02940 FOXG_03931 FOXG_07386 FOXG_09018 FOXG_11025 FOXG_13434 FOXG_14488
NHE : NECHADRAFT_29975 NECHADRAFT_30373 NECHADRAFT_51531 NECHADRAFT_52827 NECHADRAFT_54453 NECHADRAFT_80653 NECHADRAFT_92236 NECHADRAFT_93373 NECHADRAFT_98426
FFC : NCS54_00003800 NCS54_00015800 NCS54_00451500 NCS54_00664300 NCS54_00723400 NCS54_00752200 NCS54_01304000
FKR : NCS57_00001000 NCS57_00013500 NCS57_00457000 NCS57_00704200 NCS57_00763700 NCS57_00793700 NCS57_00963200 NCS57_01290500
FMU : J7337_000192 J7337_001876 J7337_007356 J7337_007407 J7337_007435 J7337_008116 J7337_008159 J7337_009271(NIT2) J7337_010993
TRE : TRIREDRAFT_64996 TRIREDRAFT_65106 TRIREDRAFT_82197
TRR : M419DRAFT_10761 M419DRAFT_85231 M419DRAFT_91336
TATV : 25780606(TrAtP1_009312) 25782869(TrAtP1_011739) 25783973(TrAtP1_002576) 25784598(TrAtP1_012113) 25784645(TrAtP1_010507) 25784714(TrAtP1_010350) 25786328(TrAtP1_012114)
TASP : 36608118(TrAFT101_008408) 36609664(TrAFT101_010587) 36614542(TrAFT101_010905) 36616661(TrAFT101_004724)
MAW : 19249271(J3458_015160) 19253907(J3458_015209)
MBRN : 26246881(NRL3) 26247494(NRL1) 26247553(NTLS)
PCHM : VFPPC_03171 VFPPC_11707
PLJ : 28889570(PLICBS_008005)
VAL : VDBG_05754 VDBG_08308
VDA : VDAG_04839 VDAG_07690 VDAG_10119
CFJ : CFIO01_00812 CFIO01_01033 CFIO01_05593 CFIO01_06272 CFIO01_09230 CFIO01_12423
CLUP : CLUP02_00498 CLUP02_02144 CLUP02_07537
CHIG : CH63R_03921 CH63R_08002 CH63R_08297 CH63R_10831 CH63R_10939 CH63R_12493
ELA : UCREL1_1388 UCREL1_1936 UCREL1_6227 UCREL1_6936 UCREL1_9592
PFY : PFICI_02756 PFICI_09266 PFICI_11864 PFICI_11917 PFICI_12363 PFICI_12386
SSL : SS1G_01487 SS1G_01652 SS1G_10174 SS1G_10951
BFU : BCIN_02g00360 BCIN_02g03010 BCIN_03g02900 BCIN_04g06870 BCIN_05g04960 BCIN_13g05670 BCIN_14g00790
PSCO : LY89DRAFT_573987 LY89DRAFT_678681 LY89DRAFT_686033 LY89DRAFT_687109
GLZ : GLAREA_02924 GLAREA_10501
ANI : ANIA_06812 ANIA_07367
AFM : AFUA_6G13450 AFUA_8G01780
ACT : ACLA_076410 ACLA_086750
NFI : NFIA_059410 NFIA_095380
AOR : AO090003000470 AO090020000385 AO090020000387 AO090020000571 AO090166000095
ANG : An01g12090 An06g01960 An08g08940 An12g01260 An16g00550 An18g01740
AFV : AFLA_001219 AFLA_010081 AFLA_010278 AFLA_011398
ALUC : AKAW2_30082A AKAW2_31391S(NIT2_2) AKAW2_40171S(NIT1_1) AKAW2_51526A AKAW2_51715A AKAW2_52056S(NIT1_2) AKAW2_70042A AKAW2_81088S
ACHE : ACHE_10130S ACHE_11010A(NIT1) ACHE_20167A(NIT2_3)
APUU : APUU_20132S(NIT1_1) APUU_40105A APUU_41671S APUU_51507A(NIT1_2) APUU_60324S(NIT2_2) APUU_70040S
PCS : N7525_001266 N7525_004429 N7525_005534 N7525_006086
PDP : PDIP_08190 PDIP_33090 PDIP_45970
POU : POX_d05365 POX_g08690
TMF : EYB26_001703 EYB26_002547
TRG : TRUGW13939_02670 TRUGW13939_06602 TRUGW13939_08592
PBL : PAAG_04639 PAAG_06015
PNO : SNOG_02380 SNOG_04183 SNOG_06401 SNOG_10152
PTE : PTT_06935 PTT_09473 PTT_10141 PTT_11435
PTRR : 6339151(PtrM4_019880) 6348868(PtrM4_079770) 6350196(PtrM4_144790) 90957214(PtrM4_123310)
BZE : COCCADRAFT_106140 COCCADRAFT_34903 COCCADRAFT_7709 COCCADRAFT_83227
BSC : COCSADRAFT_185997 COCSADRAFT_33012 COCSADRAFT_37338 COCSADRAFT_41693
BOR : COCMIDRAFT_101352 COCMIDRAFT_102937 COCMIDRAFT_30655 COCMIDRAFT_88741
AALT : CC77DRAFT_1002285 CC77DRAFT_1029262 CC77DRAFT_1080369 CC77DRAFT_201495 CC77DRAFT_82838
ARAB : EKO05_0000650 EKO05_0000949 EKO05_0005256 EKO05_0007059
ZTR : MYCGRDRAFT_43922 MYCGRDRAFT_69881
PFJ : MYCFIDRAFT_134006 MYCFIDRAFT_28493
FFU : CLAFUR5_03617 CLAFUR5_06463 CLAFUR5_09338
CBET : CB0940_03018 CB0940_05325 CB0940_08822
BCOM : BAUCODRAFT_115762 BAUCODRAFT_287107
NPA : UCRNP2_10447 UCRNP2_1515 UCRNP2_2949 UCRNP2_5691 UCRNP2_6251 UCRNP2_6530 UCRNP2_7522 UCRNP2_9073 UCRNP2_9783
KMG : 30162318(I203_100861) 30167697(I203_106797) 30168116(I203_106842) 30168229(I203_105069)
ABP : AGABI1DRAFT35296(AGABI1DRAFT_35296)
ABV : AGABI2DRAFT186144(AGABI2DRAFT_186144)
MPR : MPER_02752 MPER_09427
MRR : Moror_4663 Moror_4710 Moror_894
MORE : E1B28_002226 E1B28_010491 E1B28_010562
PSOJ : PHYSODRAFT_295379 PHYSODRAFT_472776
KPAS : LUW96_26105 LUW96_28315
GQU : AWC35_03495 AWC35_07540 AWC35_21755
RCB : O1V66_04665 O1V66_15510
BGJ : AWC36_03460 AWC36_03710
BIZ : HC231_07475 HC231_07710
LBQ : CKQ53_15000 CKQ53_16400
PAO : Pat9b_1901 Pat9b_4185 Pat9b_4815
PPHO : CTZ24_09990 CTZ24_25605
TCI : A7K98_09695 A7K98_12340
PAKH : B0X70_06225 B0X70_11505 B0X70_12325
PLUI : CE143_06145 CE143_11505 CE143_12325
XHO : A9255_03720 A9255_13125
PHEI : NCTC12003_00497(nitA)
PRJ : NCTC6933_00565(nitA)
VBR : A6E01_05890 A6E01_14295
PPSE : BN5_1632(merR1) BN5_1912(NIT4) BN5_1925 BN5_4427(NIT4B)
PNT : G5B91_15605 G5B91_33185
PCI : PCH70_14830 PCH70_24550
PPRC : PFLCHA0_c29550(nitA) PFLCHA0_c34900
PFN : HZ99_03125 HZ99_07185
PBA : PSEBR_a3130 PSEBR_a3473
PPUU : PputUW4_02136 PputUW4_02461 PputUW4_03019
PKC : PKB_3246(nita1) PKB_3594(nita3)
PALK : PSAKL28_05700 PSAKL28_22580
PFK : PFAS1_00665 PFAS1_26850 PFAS1_28105
PSIL : PMA3_12875 PMA3_13130 PMA3_17945
PUM : HGP31_16460 HGP31_17495 HGP31_19770
PSEP : C4K39_4245 C4K39_5172
PPII : QL104_16630 QL104_27480
PTRT : HU722_0013375 HU722_0014845
PAZE : KSS91_11555 KSS91_13510 KSS91_16705
PIZ : LAB08_R30840 LAB08_R33000
PNB : NK667_08445 NK667_12155
PHOM : KJF94_12075 KJF94_13100
PPAO : K3169_15530 K3169_18465
ALR : DS731_01015 DS731_20315
CEG : D0C16_01290 D0C16_10370
GAI : IMCC3135_05125 IMCC3135_18435(nitA)
HAXI : HAALTHF_26200n HAALTHF_26210n
HAAH : HALA3H3_470079(nitA) HALA3H3_740013(NIT4B)
HAA : A5892_07225 A5892_16435
ALCA : ASALC70_00429(nitA)
MARD : IBG28_18235 IBG28_18320 IBG28_18330
MFOI : JSY38_15370 JSY38_15380
RPJ : N234_03085 N234_09732 N234_21500 N234_30720 N234_36065
REH : H16_A1956(h16_A1956)
CNC : CNE_1c19260(nitA) CNE_2c20820(nit) CNE_BB1p06180(nit) CNE_BB1p06230(nitA) CNE_BB1p06270
COX : E0W60_03660 E0W60_33195
CUK : KB879_15790 KB879_22035 KB879_33405
BVI : Bcep1808_0666 Bcep1808_6052
BCED : DM42_1138(nit2) DM42_6468
BCEP : APZ15_07105 APZ15_29805 APZ15_29835
BSEM : WJ12_03130 WJ12_29720 WJ12_29775 WJ12_30785
BGL : bglu_1g01570 bglu_3p0450
BGF : BC1003_4453 BC1003_5798
BGD : bgla_1g01700 bgla_2g04420 bgla_2g12130
BGO : BM43_1566 BM43_4867 BM43_7200
BYI : BYI23_B001250 BYI23_B001940
BUE : BRPE67_BCDS03320 BRPE67_BCDS04360
BGP : BGL_1c01330 BGL_2c03350 BGL_2c09240(nitA1) BGL_2c10680(nitA2)
BPLA : bpln_1g01340 bpln_1p0470 bpln_2g03860 bpln_2g10140 bpln_2g11540
BUM : AXG89_12870 AXG89_21895 AXG89_24385 AXG89_28305 AXG89_29845
BUI : AX768_06670 AX768_24430
BURK : DM992_02770 DM992_18215 DM992_36660 DM992_39535
BXB : DR64_3321(nitA) DR64_3571
BPH : Bphy_4561 Bphy_5177 Bphy_6171
BPY : Bphyt_2715 Bphyt_4729
BCAI : K788_0001181 K788_0003234
PARA : BTO02_00325 BTO02_04855 BTO02_28980
PHS : C2L64_25980 C2L64_29890 C2L64_40025
PTER : C2L65_22405 C2L65_36050 C2L65_37265 C2L65_44220
PTRO : G5S35_22800 G5S35_33265
PEW : KZJ38_16765 KZJ38_18625 KZJ38_33955
PDIO : PDMSB3_0609(nitA) PDMSB3_3027
PKF : RW095_03515 RW095_19630
PLAD : PPGU16_36330 PPGU16_66550
CABA : SBC2_44070(nitA_1) SBC2_67270(nitA_2)
BUO : BRPE64_DCDS10080 BRPE64_DCDS11260
CABK : NK8_49830 NK8_63020
TVL : FAZ95_12085 FAZ95_27810 FAZ95_28810
BHZ : ACR54_00880(nitA) ACR54_03873 ACR54_04418
BPDZ : BBN53_01000 BBN53_17370
AXY : AXYL_01231(nit1) AXYL_01249(nit2)
AXO : NH44784_006991 NH44784_017291
AXN : AX27061_0461 AX27061_1293
AXX : ERS451415_00457(nitA_1) ERS451415_01288(nitA_2)
ADT : APT56_02415 APT56_05965
AIS : BUW96_04600 BUW96_20015 BUW96_20110
ACHR : C2U31_07085 C2U31_11725
ADY : HLG70_16220 HLG70_16400
APES : FOC84_06480 FOC84_06710
AAEG : RA224_26600 RA224_26695
PUD : G9Q38_03175 G9Q38_04385
PUS : CKA81_10465 CKA81_10470
AFA : UZ73_10375 UZ73_12600
AAQU : D3M96_00270 D3M96_18000
AAMM : FE795_00245 FE795_15545
PIG : EGT29_10520 EGT29_26885
PACR : FXN63_05010 FXN63_25690
RHY : RD110_19125 RD110_25810
VAP : Vapar_0561 Vapar_0653 Vapar_2541
VPE : Varpa_0597 Varpa_3385
VPD : VAPA_1c06370(nitA) VAPA_1c30110 VAPA_2c12380
VBO : CKY39_15765 CKY39_21885
VAM : C4F17_06945 C4F17_15915
METP : C1M51_04285 C1M51_07255
ATER : MW290_07530 MW290_12770
HRB : Hrubri_1097(nitA) Hrubri_2894 Hrubri_3574 Hrubri_3580(nitA) Hrubri_4385 Hrubri_4424
AZR : CJ010_15460 CJ010_16130
EMX : FKV68_28560 FKV68_28790
RII : FFM53_009865 FFM53_032150
RBQ : J2J99_03815 J2J99_32125
RRG : J3P73_03880 J3P73_35505
ARA : Arad_12186 Arad_12224 Arad_12225 Arad_8067
BJA : bll6402(bll6402) blr3397(nit)
BJU : BJ6T_30000 BJ6T_45630 BJ6T_62410
BJP : RN69_14645 RN69_22150 RN69_30245
BRS : S23_10580 S23_17900 S23_30640
BRK : CWS35_21570 CWS35_24250 CWS35_30190
BGZ : XH91_09905 XH91_15370
BEL : BE61_22870 BE61_49640 BE61_57570
BSEP : HAP48_0038315 HAP48_0042045
BQB : J4P68_0002285 J4P68_0014010 J4P68_0039165
BAUT : QA635_06690 QA635_19800 QA635_22490
BBRA : QA636_06465 QA636_20175 QA636_23045 QA636_25770
BCOU : IC761_05495 IC761_15565
BPAH : QA639_06340 QA639_22510 QA639_25195
BXN : I3J27_13605 I3J27_22265
BRAZ : LRP30_23260 LRP30_41545
BOY : LQG66_02470 LQG66_05100 LQG66_08190
RPA : TX73_008030 TX73_021595
VGO : GJW-30_1_02102(nitA) GJW-30_1_04098
BVES : QO058_05400 QO058_29630
XDI : EZH22_00110 EZH22_02740 EZH22_21175
APRA : G3A50_11335 G3A50_13815
MEA : Mex_1p0252 Mex_1p0929 Mex_2p1117 Mex_2p1120
MMES : MMSR116_00720 MMSR116_00750
HMC : HYPMC_3034 HYPMC_3916
FIL : BN1229_v1_1636 BN1229_v1_1717
FIY : BN1229_v1_1639 BN1229_v1_1720
MMYR : MXMO3_02584 MXMO3_02972 MXMO3_03232
PSIN : CAK95_03825 CAK95_20925 CAK95_28685
LAGG : B0E33_09910 B0E33_19585
YPAC : CEW88_08565 CEW88_19585
YAN : AYJ57_17480 AYJ57_17515 AYJ57_18340
RMM : ROSMUCSMR3_04023(nitA)
PPRU : FDP22_11110 FDP22_16920
NPP : PP1Y_Mpl8238 PP1Y_Mpl8474
NHUM : PQ457_19775 PQ457_20940 PQ457_21950
SWI : Swit_0832 Swit_0987 Swit_2469 Swit_4178
SPHD : HY78_21575 HY78_22535
PARJ : J4G78_01470 J4G78_09875
PHAO : HF685_02455 HF685_12130
POQ : KZX46_06645 KZX46_12970
EMV : HQR01_04175 HQR01_10800
GOH : B932_2218 B932_3190 B932_3233 B932_3560
KOI : LV478_00285 LV478_01220
ASZ : ASN_2364(nit) ASN_3201(nitA)
APER : A0U91_01625 A0U91_11055
AOT : AcetOri_orf00637 AcetOri_orf04209
AOY : EOV40_011580 EOV40_013410 EOV40_014145 EOV40_014305
TMO : TMO_b0445 TMO_c0200(nitA)
HFL : PUV54_02265 PUV54_03995
PAQI : KW060_05865 KW060_11080
CFUS : CYFUS_000174 CYFUS_005834 CYFUS_008412
SCU : SCE1572_09890 SCE1572_51170
BSM : BSM4216_0250 BSM4216_0628
BFX : BC359_07360 BC359_18825
PTB : DER53_11320 DER53_11790
APAK : AP3564_00495 AP3564_01485
ACOP : RI196_16015 RI196_16975
PNP : IJ22_32890 IJ22_33550
MSM : MSMEG_1120 MSMEG_4138 MSMEG_5373
MSG : MSMEI_1088 MSMEI_4039 MSMEI_5227
MSB : LJ00_05570 LJ00_20520 LJ00_26555
MSN : LI99_05570 LI99_20525 LI99_26560
MSH : LI98_05570 LI98_20530 LI98_26565
MTY : MTOK_06500 MTOK_36460
MTER : 4434518_00326(nitA)
MHIB : MHIB_11990 MHIB_38620
NFR : ERS450000_00807(nitA)
NSL : BOX37_04110 BOX37_04125
NSR : NS506_07490 NS506_07492
NOD : FOH10_26435 FOH10_26450
NAD : NCTC11293_01652 NCTC11293_03373
NHU : H0264_06190 H0264_06200
NYA : LTV02_26675 LTV02_26685
NYU : D7D52_25550 D7D52_25560
ROA : Pd630_LPD03776 Pd630_LPD07594
RHOJ : JVH1_20650 JVH1_37660 JVH1_37735
SXT : KPP03845_106488(nitA)
ACRY : AC20117_15300 AC20117_22230
ASUF : MNQ99_07100 MNQ99_07105
ARHD : VSH64_16585 VSH64_25255 VSH64_38710
PBRO : HOP40_02680 HOP40_17625
PSDS : K1T35_06035 K1T35_36945
SESP : BN6_38440 BN6_40650
KUT : JJ691_02330 JJ691_09660 JJ691_24980 JJ691_96480
UME : RM788_04275 RM788_46280
DACT : KZZ52_15000 KZZ52_44375
SBAE : DSM104329_00229(nitA_2)
LET : O77CONTIG1_03060(nitA)
SCYS : NIES4073_23250(merR)
KBS : EPA93_16070 EPA93_39425
GAW : V144x_14930 V144x_47600
LRS : PX52LOC_03589 PX52LOC_04805
SPIB : G8759_29580 G8759_31815
SENF : GJR95_23500 GJR95_33280
RSR : T7867_01895 T7867_18000
FSD : LXD69_00055 LXD69_15390
MART : BTR34_00830 BTR34_17240
PHG : KCTC32516_01151(nitA)
KOS : KORDIASMS9_00587(nitA)
KAN : IMCC3317_24390(nitA)
AQD : D1816_07700 D1816_19810
ASAG : FGM00_00415 FGM00_16040
FLB : LV704_11070 LV704_15755
FBQ : D1817_10185 D1817_14910
LOKI : Lokiarch_49440(nitA)
» show all
Taxonomy
Reference
Authors
Harper DB.
Title
Microbial metabolism of aromatic nitriles. Enzymology of C-N cleavage by Nocardia sp. (Rhodochrous group) N.C.I.B. 11216.
Journal
Reference
Authors
THIMANN KV, MAHADEVAN S.
Title
NITRILASE. I. OCCURRENCE, PREPARATION, AND GENERAL PROPERTIES OF THE ENZYME.
Journal
Reference
Authors
Pace HC, Brenner C.
Title
The nitrilase superfamily: classification, structure and function.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 3.5.5.1
ExPASy - ENZYME nomenclature database: 3.5.5.1
UM-BBD (Biocatalysis/Biodegradation Database): 3.5.5.1
BRENDA, the Enzyme Database: 3.5.5.1
LinkDB
All DBs