KEGG ENZYME: 3.7.1.17

ENZYME: 3.7.1.17

Entry

EC 3.7.1.17                 Enzyme

Name

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase;
tesD (gene name);
hsaD (gene name)

Class

Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances

Sysname

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming)

Reaction(IUBMB)

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H2O = 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate [RN:R09883]

Reaction(KEGG)

R09883

Substrate

(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate [CPD:C04844];
H2O [CPD:C00001]

Product

3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate [CPD:C20199];
(2Z,4Z)-2-hydroxyhexa-2,4-dienoate [CPD:C20240]

Comment

The enzyme is involved in the bacterial degradation of the steroid ring structure, and is involved in degradation of multiple steroids, such as testosterone [1], cholesterol [2], and sitosterol.

History

EC 3.7.1.17 created 2012

Pathway

ec00984

Steroid degradation

Orthology

K16050

4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

Genes

RGL:	CS053_01755

VAF:	D1115_22495

PRE:	PCA10_13810

PFUW:

KF707C_12160

PLAL:

FXN65_06415

PPUT:

L483_14175

PPUN:

PP4_27040(mhpC)

PVR:	PverR02_11710

PPUU:

PputUW4_01659(mhpC)

PKC:

PKB_4240

PFZ:	AV641_10660

PLQ:	AA042_19905

PPSY:

AOC04_07965

PSET:

THL1_1432

PSIL:

PMA3_11770

PKE:	DLD99_14450 DLD99_14755

PUM:	HGP31_20725

PPSH:

G5J76_13915

PSEP:

C4K39_5448

PMUY:

KSS95_17290

PAZE:

KSS91_19300

PTAE:

NCTC10697_02081(mhpC)

PCAV:

D3880_08220 D3880_17375

PPHN:

HU825_14570

PIZ:	LAB08_R40980

PFIT:

KJY40_13695

PKM:	PZ739_11655

PTRL:

OU419_14680

PSHS:

JJN09_05420

PLAU:

I0D68_06185

PFOU:

OZ911_14190

PBEJ:

PSH84_15825

PSKP:

KU43P_19430(hsaD)

PDEU:

QEP73_19105

PBUB:

V6B39_14530

PSML:

V6P94_14155

PSMZ:

V6L79_14340

SDEG:

GOM96_01565

HARR:

HV822_17815

PPHA:

BVH74_17745

HASM:

QAO71_12665

KAK:	Kalk_07850

ASEI:

I8T81_09205

ACUM:

C9E88_011925

ADS:	FPL17_05420

ATN:	FM020_06325

ACOT:

QLH32_00225

SPL:

Spea_2147

SHL:

Shal_2118

SHEM:

HWQ47_19740

COLW:

A3Q33_18715

CBER:

B5D82_11110

PHA:	PSHAa0893(tesD)

PTN:	PTRA_a1026(hsaD)

PNG:	PNIG_a1077(hsaD)

SNAN:

I6N98_01455 I6N98_10805

ZAL:	AZF00_11435

MSPN:

M8T91_14355

HJA:	BST95_09500

HALC:

EY643_13300

AFUS:

EYZ66_12960

PAQM:

E0F26_07480

MSEC:

LN244_05065

MRZ:	KDW95_01070

EMP:	EZMO1_1316(tesD)

SOK:	D0B54_04210 D0B54_06205

SINI:

GT972_14675

FCE:	JN531_005825

GBI:	PG2T_02180

GPB:	HDN1F_27100(tesD)

RIN:	ACS15_4349

REH:	H16_B0600(bphD)

CNC:	CNE_2c05450(fabG2)

CUH:	BJN34_30955

REU:	Reut_A1513

CTI:	RALTA_B0404(mhpC)

CBW:	RR42_s2308

COX:	E0W60_08040

CUK:	KB879_20440

CUPP:

CTP10_R38500

BHG:	I6G56_07560

BUD:	AQ610_12660

BUR:	Bcep18194_B1463

BCJ:

BCAM1609

BCEN:

DM39_3387

BCEO:

I35_5469

BCT:

GEM_4115

BCED:

DM42_6349

BCEP:

APZ15_31175

BPYR:

ABD05_27840

BCON:

NL30_04160

BDF:	WI26_21125

BTEI:

WS51_05675

BPSL:

WS57_06065

BMEC:

WJ16_19990 WJ16_26770

BSTL:

BBJ41_21320 BBJ41_34095

BANN:

JFN94_25630

BARI:

NLX30_30205 NLX30_35010

BAEN:

L3V59_21720

BUL:	BW21_2676(hsaD)

BPLA:

bpln_1g12680

BURK:

DM992_32645

BPH:

Bphy_3515

BFN:

OI25_4585

PBRY:

NDK50_32855

PPAI:

E1956_37260

PVE:	UC34_07060

PFIB:

PI93_021410

CABA:

SBC2_49060(mhpC)

BGV:	CAL12_23370

AVH:	WHX56_15225

CTT:	CtCNB1_1354

CTES:

O987_07745

CRJ:	QMY55_07025

DIH:	G7047_09710

SMIO:

CATMQ487_29910(mhpC)

AZD:	CDA09_09460

THU:	AC731_008590

THAU:

C4PIVTH_4256

THAG:

CKCBHOJB_02331(mhpC)

CAUL:

KCG34_11045

CON:	TQ29_16150

SSTL:

ABFK29_22620

PALX:

GQA70_21455

FRB:	AB6B37_08190

NAR:

Saro_3690

NPP:	PP1Y_Mpl3096

NOV:	TQ38_027285

NOT:	C7W88_20930

NOVO:

IM701_15595

SPOG:

EEB18_002705

SWI:	Swit_1754 Swit_1827 Swit_3338 Swit_4183

SPHD:

HY78_08860 HY78_16445 HY78_16815

RDI:	CMV14_03930 CMV14_16820

SSAN:

NX02_12715

SPAN:

AWL63_19985

SPKC:

KC8_19645

SPHA:

D3Y57_15210

SLAN:

GV829_01865

SBIN:

SBA_ch2_4310

SNAP:

PQ455_10975

SPHB:

EP837_03392(hsaD)

SPCA:

GL174_15760

PHAO:

HF685_05525

PHZ:	CHX26_05310

NTD:	EGO55_04995 EGO55_13685

CAEN:

K5X80_14730

SEDD:

ERJ70_09520

AACO:

K1I37_05675

MTU:	Rv3569c(hsaD)

MTV:	RVBD_3569c

MTC:	MT3674(bphD)

MRA:	MRA_3608(bphD)

MTF:	TBFG_13602

MTB:	TBMG_03608

MTK:	TBSG_03635

MTZ:	TBXG_003583

MTG:	MRGA327_22035

MTI:	MRGA423_22555

MTE:	CCDC5079_3310

MTUR:

CFBS_3786(bphD)

MTL:	CCDC5180_3262

MTO:	MTCTRI2_3634(bphD)

MTD:	UDA_3569c(bphD)

MTN:	ERDMAN_3916(bphD)

MTJ:	J112_19220

MTUB:

MT7199_3632

MTUC:

J113_24995

MTUE:

J114_19090

MTX:	M943_18355

MTUH:

I917_25075

MTUL:

TBHG_03510

MTUT:

HKBT1_3771(bphD)

MTUU:

HKBT2_3780(bphD)

MTQ:	HKBS1_3783(bphD)

MBO:	BQ2027_MB3600C(hsad)

MBB:	BCG_3634c(bphD)

MBT:	JTY_3635(bphD)

MBM:	BCGMEX_3632c(bphD)

MBK:	K60_037070

MBX:

BCGT_3436

MAF:	MAF_35820(bphD)

MMIC:

RN08_3946

MCE:	MCAN_35821(bphD)

MCQ:	BN44_110064(bphD)

MCV:	BN43_90070(bphD)

MCX:	BN42_90067(bphD)

MCZ:	BN45_100083(bphD)

MORY:

MO_003728

MPA:

MAP_0498

MAO:

MAP4_3369

MAVI:

RC58_16710

MAVU:

RE97_16745

MAV:	MAV_0592 MAV_2517

MAVM:

MAA44156_00511(hsaD_1)

MIT:

OCO_05010

MIA:

OCU_05050

MID:

MIP_00941

MYO:

OEM_05070

MCHI:

AN480_02500

MIR:

OCQ_05160

MMAL:

CKJ54_02430

MLP:

MLM_0736

MMAN:

MMAN_47950(bphD)

MSER:

MTY59_24290(bphD)

MSA:	Mycsm_01915 Mycsm_01992 Mycsm_05839

MUL:	MUL_4140(bphD)

MMI:	MMAR_1502 MMAR_5064(bphD)

MMAE:

MMARE11_14020 MMARE11_48790(bphD)

MLI:	MULP_01620 MULP_05322(bphD)

MPSE:

MPSD_43530 MPSD_52650(hsaD)

MSHO:

MSHO_05480(bphD) MSHO_21750

MMC:

Mmcs_4708

MKM:

Mkms_4794

MJL:

Mjls_5093

MMM:

W7S_02445

MKN:	MKAN_12125 MKAN_22320

MYV:	G155_26480

MYE:	AB431_09620

MHAD:

B586_04450

MDX:	BTO20_04380 BTO20_24345

MSHG:

MSG_03543 MSG_04562(hsaD)

MFJ:	MFLOJ_40660(hsaD)

MSTO:

MSTO_44610(hsaD) MSTO_55690

MSIM:

MSIM_29100(hsaD)

MSAK:

MSAS_13430 MSAS_29160(hsaD)

MKU:	I2456_25680

MLW:	MJO58_19440 MJO58_25095

MXE:	MYXE_05070(bphD)

MNV:	MNVI_12520(bphD)

MPAG:

C0J29_01145 C0J29_27830

MGOR:

H0P51_01140 H0P51_25870

MCOO:

MCOO_27320(bphD) MCOO_46610(mhpC_3)

MBAI:

MB901379_04453(hsaD_3)

MSEO:

MSEO_27510 MSEO_34560(bphD)

MHEK:

JMUB5695_00468(bphD)

MGAU:

MGALJ_07320 MGALJ_38570

MLJ:	MLAC_47230(bphD)

MBRD:

MBRA_01310 MBRA_20000(hsaD)

MSHJ:

MSHI_38530(hsaD)

MMAM:

K3U93_22690

MSPG:

F6B93_20490

MOT:	LTS72_27915

MPAA:

MKK62_02390 MKK62_17500

MKY:	IWGMT90018_57500(bphD)

MADI:

A7U43_06660 A7U43_08590

MWU:	PT015_00105 PT015_15565

MYW:	BVC93_09280

MYJ:	BCA37_02630

MSM:	MSMEG_6037

MSG:	MSMEI_5876(bphD)

MSB:	LJ00_29845

MSN:	LI99_29850

MSH:	LI98_29855

MVA:	Mvan_0592 Mvan_5307

MGI:

Mflv_1464

MSP:	Mspyr1_47030

MCB:	Mycch_4674

MNE:	D174_24705

MYN:	MyAD_24240

MFT:	XA26_21680 XA26_53620

MPHL:

MPHLCCUG_00492(hsaD_1)

MVQ:

MYVA_5217

MLL:	B1R94_19690 B1R94_25465

MRH:	MycrhN_2244

MTHN:

4412656_04093(hsaD_3)

MHAS:

MHAS_03778(hsaD_5)

MDU:	MDUV_49000

MCHT:

MCHIJ_19300

MAUU:

NCTC10437_05068(hsaD_3)

MMAG:

MMAD_48580

MMOR:

MMOR_11750

MFX:	MFAL_03550(mhpC) MFAL_32590

MAIC:

MAIC_16360

MIJ:	MINS_00700

MALV:

MALV_42130

MTY:	MTOK_21990

MPSC:

MPSYJ_15760

MARZ:

MARA_47430

MGAD:

MGAD_37690

MHEV:

MHEL_27640 MHEL_40900

MSAR:

MSAR_10980

MANY:

MANY_02510(mhpC)

MAUB:

MAUB_47460(hsaD)

MPOF:

MPOR_54000

MPHU:

MPHO_21610 MPHO_43370

MMUC:

C1S78_007415 C1S78_025615

MMAT:

MMAGJ_33770

MBOK:

MBOE_04970 MBOE_53390

MFG:	K6L26_05340

MSEI:

MSEDJ_02520

MFLV:

NCTC10271_00466(hsaD_1)

MCEE:

MCEL_37730

MMON:

EWR22_25465

MGO:	AFA91_10585

MHOL:

K3U96_03075

MSEN:

K3U95_25515

MPAE:

K0O64_09000

MRF:	MJO55_02070 MJO55_24180

MDF:	K0O62_25970

MCRO:

MI149_09950

MGRO:

FZ046_02945

MAUS:

JN090_01850 JN090_26585

MFRE:

EXE63_18690

MBUG:

MU0053_000394

MIZ:	BAB75_13835

MJD:	JDM601_1132

MMIN:

MMIN_05410(mhpC_1)

MHER:

K3U94_06260

MVM:	MJO54_06430

MKK:	MU0083_001771

ASD:	AS9A_0925(mhpC)

COK:	COCCU_00295(hsaD1)

NFA:

NFA_4730

NFR:	ERS450000_03763(hsaD_1)

NCY:	NOCYR_0502

NBR:	O3I_002670

NNO:	NONO_c05020(hsaD) NONO_c29060 NONO_c37010

NSL:	BOX37_02180

NSR:	NS506_01261(hsaD) NS506_06808

NTP:	CRH09_03310

NOZ:	DMB37_23595

NOD:	FOH10_28740

NAH:	F5544_02635

NAD:	NCTC11293_01246(hsaD_2) NCTC11293_04256(hsaD_4)

NWL:	NWFMUON74_04940

NIE:	KV110_03030

NTC:	KHQ06_35200

NHU:	H0264_03855

NYA:	LTV02_28885 LTV02_38600

NGP:	LTT66_00665 LTT66_14745 LTT66_15425

NSPU:

IFM12276_01690 IFM12276_39830

NYU:	D7D52_04500 D7D52_09565

NVU:	V7968_10090(hsaD)

RHA:	RHA1_ro04540(hsaD)

RER:

RER_07560

REY:

O5Y_03675

REB:	XU06_03915

RQI:	C1M55_04155

ROP:

ROP_44550

ROA:	Pd630_LPD00992

RPY:	Y013_19030

RBY:	CEJ39_05395

RAV:	AAT18_03970 AAT18_26195

RHW:	BFN03_15195

RRZ:	CS378_19610

RHQ:	IM25_08880

RHOD:

AOT96_14550

RRT:	4535765_00583(hsaD_1) 4535765_04568(hsaD_4)

RCR:	NCTC10994_02137(hsaD_2)

RTM:	G4H71_07390

RKO:	JWS14_24490

RGO:	KYT97_23240

ROZ:	CBI38_04175

RPSK:

JWS13_20435

RGOR:

NMQ04_02520 NMQ04_22045 NMQ04_22210

RHOP:

D8W71_26910

RHOO:

RA302_04025

RHOU:

KZJ41_03965 KZJ41_22865 KZJ41_27645

RHOW:

A4U64_20805 A4U64_25020

RHOJ:

JVH1_13630(hsaD)

RHOS:

QWW67_16860 QWW67_21310

RHOQ:

QWW98_16860 QWW98_21320

RHOY:

QNA09_01520

RHOH:

CPI83_25060

RHOX:

RBB84_23650

RHJS:

OYT95_14225

REQ:	REQ_01450 REQ_06810 REQ_35100

PDEF:

P9209_02565 P9209_05880 P9209_29070

PSOW:

ABI214_10415(hsaD) ABI214_13280

GOJ:	GR168_23130

GAM:	GII34_11030 GII34_18525

GPD:	GII33_04205 GII33_08475 GII33_16055

GMG:	NWF22_15540

SPIN:

KV203_17345 KV203_17540

TPR:

Tpau_3844

TSM:	ASU32_13590 ASU32_21900

TPUL:

TPB0596_41850 TPB0596_45490

TSD:	MTP03_43290

DTM:	BJL86_2839 BJL86_3316

DIT:	C3V38_06170

DIZ:	CT688_16945

DPC:	A6048_17575

DLU:	A6035_16880

DKN:	NHB83_17160

DCD:	VYL96_16585

TOY:	FO059_01795

SCYE:

R2B67_01940

SMAL:

SMALA_0968 SMALA_1212

SSOI:

I1A49_08330 I1A49_09545

SDV:	BN159_8214

SVT:	SVTN_35910

SLC:	SL103_24910

STSI:

A4E84_36410

SGM:	GCM10017557_80450(mhpC)

SGRS:

V9K83_03335

SALU:

DC74_7446

SALL:

SAZ_38435

SAUO:

BV401_08635 BV401_09835

SALF:

SMD44_02218(hsaD)

SLX:	SLAV_27435(hsaD)

SAQU:

EJC51_46900

SPHV:

F9278_45575

SCHA:

CP983_10625

SGX:	H4W23_38545

SSPB:

CP982_11900

SNF:	JYK04_02662(hsaD)

SVD:	CP969_28370

SPRA:

CP972_28850

SXN:	IAG42_26650

SLF:	JEQ17_44910

SDEC:

L3078_04210 L3078_31100

SAOV:

G3H79_28030

STAA:

LDH80_36405

SNIG:

HEK616_31470(mhpC)

SCIR:

STRCI_000454

SLON:

LGI35_13840

SKG:	KJK29_37290

SFP:	QUY26_30060

STII:

DRB96_33810

STIC:

DRB89_33170

SMOD:

POD33_23325

YIA:	LO772_04905

MIO:	AOA12_18570

SALD:

FVA74_01355

ASUF:

MNQ99_15000

JTE:	ASJ30_14570

KNO:	V6K52_03630

NCA:

Noca_2775

NDK:	I601_3469(hsaD_2)

NBE:	Back2_27210(mhpC_1)

NANO:

G5V58_09765

NARO:

CFH99_13525

NPI:	G7071_04055 G7071_15940

NPC:	KUV85_04285

NMAR:

HPC71_01600 HPC71_09520

NFC:	KG111_01290 KG111_05690 KG111_08320

NBI:	SHK19_12520

NOF:	MF408_21030

NOCR:

OSR43_09580

NOCW:

ABFU82_07550(hsaD) ABFU82_20390

NOCA:

ncot_09680

NOCL:

KM427_12560 KM427_25145

NOCD:

QJ852_14495 QJ852_20515

NOCQ:

QI633_11900

PSIM:

KR76_14475

AEB:	C6I20_03265

AWN:	NQV15_16590

AMAZ:

LUW76_09950 LUW76_09965 LUW76_45470

ACIR:

OG979_00780

ACTQ:

OG417_51275

NFS:	OIE67_46760

FRI:	FraEuI1c_4100

AMD:	AMED_2523(mhpC) AMED_7185

AMN:	RAM_12825 RAM_36905

AMM:	AMES_2495(mhpC) AMES_7076

AMZ:	B737_2496(mhpC) B737_7076

AOI:	AORI_2485(mhpC)

AJA:	AJAP_26400(hsaD)

AMQ:	AMETH_4837(mhpC)

AMYC:

CU254_10465

AMYB:

BKN51_05025

AAB:	A4R43_16040

AMYY:

YIM_14455(hsaD1)

AORI:

SD37_13490

AACD:

LWP59_08655 LWP59_29195

APRT:

MUY14_23825

AROO:

NQK81_09925

ATHM:

L1857_26960

ARHD:

VSH64_01785 VSH64_09275

AMOG:

QRX60_26345

ACYN:

ORV05_07255 ORV05_08280

ACOR:

LCL61_01250(hsaD)

AMYZ:

HUW46_00281(hsaD_1)

ANW:	QP939_46085 QP939_49825

AMYD:

K1T34_30670

ABAR:

AMYBAR_003326 AMYBAR_003332 AMYBAR_005562

AMYE:

MJQ72_04365

AMYS:

CVV72_16690

PBRO:

HOP40_22535

PPEL:

H6H00_19080

PSDS:

K1T35_06300 K1T35_10825

KAL:

KALB_5123

KUT:	JJ691_08340(mhpC_1) JJ691_37190(mhpC_2)

KBR:	Q4V65_18925 Q4V65_43210(hsaD)

KPHY:

AOZ06_42785

LED:	BBK82_38920

ALO:

CRK59593

PMAD:

BAY61_10960

SACE:

GIY23_13335

CROS:

N8J89_29370

STP:	Strop_2670

SAQ:

Sare_2878

MCRA:

ID554_07075

VER:	HUT12_12175

AMS:	AMIS_19190

AIH:	Aiant_18880(mhpC)

ACTL:

L3i22_084460(mhpC)

ASIC:

Q0Z83_065580

AOU:	ACTOB_004120

ACTE:

ACTI_41220(mhpC)

AFS:

AFR_13045

DFU:	Dfulv_33635

LMAJ:

GKN94_12655

» show all

Reference

1 [PMID:16151114]

Authors

Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T

Title

Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.

Journal

Appl Environ Microbiol 71:5275-81 (2005)
DOI:10.1128/AEM.71.9.5275-5281.2005

Reference

2 [PMID:17264217]

Authors

Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Dijkhuizen L, Davies JE, Mohn WW, Eltis LD

Title

A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.

Journal

Proc Natl Acad Sci U S A 104:1947-52 (2007)
DOI:10.1073/pnas.0605728104

Sequence

[mtu:Rv3569c]