Entry Name phenylacetate decarboxylase;
Class Lyases;BRITE hierarchy
Sysname phenylacetate carboxy-lyase
Reaction(IUBMB) phenylacetate = toluene + CO2 [RN:
R12504 ]
Reaction(KEGG) Substrate Product Comment This bacterial enzyme, isolated from anoxic, toluene-producing microbial communities, is a glycyl radical enzyme. It needs to be activated by a dedicated activating enzyme (PhdA). The activase catalyses the reductive cleavage of AdoMet, producing a 5'-deoxyadenosyl radical that leads to the production of the glycyl radical in PhdB.
History EC 4.1.1.119 created 2019
Reference Authors Zargar K, Saville R, Phelan RM, Tringe SG, Petzold CJ, Keasling JD, Beller HR
Title In vitro Characterization of Phenylacetate Decarboxylase, a Novel Enzyme Catalyzing Toluene Biosynthesis in an Anaerobic Microbial Community.
Journal Reference Authors Beller HR, Rodrigues AV, Zargar K, Wu YW, Saini AK, Saville RM, Pereira JH, Adams PD, Tringe SG, Petzold CJ, Keasling JD
Title Discovery of enzymes for toluene synthesis from anoxic microbial communities.
Journal Sequence Reference Authors Rodrigues AV, Tantillo DJ, Mukhopadhyay A, Keasling JD, Beller HR.
Title Insight into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene-Producing Glycyl Radical Enzyme.
Journal Other DBs ExPASy - ENZYME nomenclature database: 4.1.1.119
LinkDB All DBs
