KEGG ENZYME: 4.1.99.20

ENZYME: 4.1.99.20

Entry

EC 4.1.99.20                Enzyme

Name

3-amino-4-hydroxybenzoate synthase;
3,4-AHBA synthase;
griH (gene name)

Class

Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases

Sysname

2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate hydro-lyase (cyclizing, 3-amino-4-hydroxybenzoate-forming)

Reaction(IUBMB)

2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate = 3-amino-4-hydroxybenzoate + phosphate + 2 H2O [RN:R10469]

Reaction(KEGG)

R10469

Substrate

2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate [CPD:C20644]

Product

3-amino-4-hydroxybenzoate [CPD:C12115];
phosphate [CPD:C00009];
H2O [CPD:C00001]

Comment

Requires Mn2+ for maximum activity. The reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate. In the bacterium Streptomyces griseus the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore.

History

EC 4.1.99.20 created 2013, modified 2016

Pathway

ec00997

Biosynthesis of various other secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K17835

3-amino-4-hydroxybenzoic acid synthase

Genes

PAKH:

B0X70_03100

PLUI:

CE143_03085

POJ:	PtoMrB4_11000

PTW:	TUM18999_11970

PSOA:

PSm6_31310

MII:	BTJ40_01850

CRZ:	D1345_21920

BUR:	Bcep18194_C6863

BCEP:

APZ15_37510

BSTL:

BBJ41_35550

BUZ:	AYM40_17235

PBH:	AAW51_0199

MANC:

IV454_17570

SUR:	STAUR_3233 STAUR_6099

AVM:	JQX13_12295

CFUS:

CYFUS_007180

CCRO:

CMC5_017700(aroB)

BWH:	A9C19_08405

MEKU:

HUW50_13325 HUW50_25370

MLIT:

KDJ21_015685

MENL:

MVE64_03150

MEBZ:

LPC09_15060

PCHI:

PC41400_02280

PTJ:	JRJ22_24805

CCE:

Ccel_2374

DDL:	Desdi_3050

HMO:	HM1_1788(aroB)

SINT:

K5I26_08865

MCHT:

MCHIJ_41760 MCHIJ_41820

NIE:	KV110_28195(griH) KV110_32285

RTM:	G4H71_04125

RGO:	KYT97_13025

GPO:	GPOL_c23160

GOM:	D7316_02314(griH)

GAM:	GII34_12235

GPD:	GII33_11545

GOI:	LK459_04840

GMG:	NWF22_16000

GSI:	P5P27_03670

SGR:	SGR_4248(griH)

SANL:

KZO11_09790 KZO11_14635(griH) KZO11_24550

SGRF:

SGFS_035540 SGFS_074010 SGFS_087240

SCYE:

R2B67_21115(griH)

SCB:	SCAB_69861

SRC:	M271_02965

SBH:

SBI_01605

SVE:

SVEN_5125

SVT:	SVTN_26395

SLD:

T261_2096

SPRI:

SPRI_1930

STSI:

A4E84_17115

SLS:	SLINC_4427 SLINC_5464

SNR:	SNOUR_07210(griH)

SPLU:

LK06_031740

SGV:	B1H19_08410

SFK:

KY5_6733

SLK:	SLUN_21420

SKA:	CP970_06990

SGAL:

CP966_31005

SPAD:

DVK44_19835

STUI:

GCM10017668_29370

SFEU:

IM697_30795

SDW:	K7C20_14900

SROI:

IAG44_38355

SMAO:

CAG99_20770

SLF:	JEQ17_24460

SDEC:

L3078_11310

SAKB:

K1J60_34230

SCAE:

IHE65_08590(griH) IHE65_34335

SLON:

LGI35_32535

SANT:

QR300_02500

SJN:	RI060_03960

SHAU:

K9S39_40190

SKG:	KJK29_03010

SCOA:

QU709_14190 QU709_20150

SLAI:

P8A22_17060

SLUO:

PS467_03245

STRI:

C7M71_007295

STRH:

GXP74_28275

ABRY:

NYE86_23065

MIO:	AOA12_03110

MPH:

MLP_27050

FRA:	Francci3_2069 Francci3_4206

SEN:

SACE_5077

SACG:

FDZ84_06735 FDZ84_27220

AROO:

NQK81_43520

ACOR:

LCL61_30705

AMYZ:

HUW46_08674(griH)

SESP:

BN6_21370(griH1) BN6_63890(griH2)

SSYI:

EKG83_36320

SACX:

IOD16_25780

KAL:

KALB_2250

KUT:	JJ691_51000

KPHY:

AOZ06_26450

ACTU:

Actkin_05212(griH)

UME:	RM788_01325 RM788_07315

MICB:

MicB006_2903

MCAB:

HXZ27_13405

MSAG:

GCM10017556_11890

MCHL:

PVK74_09470

ACTN:

L083_5378

ACTE:

ACTI_84870

PFLA:

Pflav_059800

PSUU:

Psuf_020550 Psuf_091140

DVC:	Dvina_29175 Dvina_40325

DFU:	Dfulv_30525

DROS:

Drose_22870

DMAT:

Dmats_24415 Dmats_35185

DACT:

KZZ52_19495

GLY:	K3N28_00695

MPRO:

BJP34_11735

» show all

Reference

1 [PMID:17003031]

Authors

Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S

Title

Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.

Journal

J Biol Chem 281:36944-51 (2006)
DOI:10.1074/jbc.M608103200

Sequence

[sgr:SGR_4248]

Other DBs

ExplorEnz - The Enzyme Database:

4.1.99.20

IUBMB Enzyme Nomenclature:

4.1.99.20

ExPASy - ENZYME nomenclature database:

4.1.99.20

BRENDA, the Enzyme Database:

4.1.99.20

LinkDB

DBGET integrated database retrieval system