KEGG ENZYME: 4.2.1.178

ENZYME: 4.2.1.178

Entry

EC 4.2.1.178                Enzyme

Name

difructose-dianhydride-III synthase;
DFA-IIIase;
difructose anhydride III hydrolase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

inulobiose hydro-lyase (alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride-forming)

Reaction(IUBMB)

inulobiose = alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O [RN:R12927]

Reaction(KEGG)

R12927

Substrate

inulobiose [CPD:C01711]

Product

alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride [CPD:C04420];
H2O [CPD:C00001]

Comment

The enzyme participates in an inulin degradation pathway, in which it forms inulobiose from difructose anhydride III. A conformational change in the enzyme from the bacterium Pseudarthrobacter chlorophenolicus results in it also catalysing the activity of EC 4.2.2.18, inulin fructotransferase (DFA-III-forming).

History

EC 4.2.1.178 created 2021

Orthology

K25596

difructose-dianhydride-III synthase

Genes

KVA:

Kvar_5014

KPK:	A593_11155

KVD:	KR75_08560

KOX:

KOX_07155

KOE:

A225_0068

KOY:	J415_02590

KOM:	HR38_05695

KOK:	KONIH1_00335

KOC:	AB185_06645

KGR:	JJJ10_27210

KPAS:

LUW96_07080

KLC:	K7H21_27435

KLM:	BWI76_00635

KLP:	GUC22_26610

ROR:	RORB6_18285

RON:	TE10_07105

RPLN:

B1209_26505

KLU:	K7B04_01090

LER:	GNG29_05760

KIN:	AB182_13660

SRL:	SOD_c28250

SRY:	M621_15425

SERM:

CLM71_06785

SFJ:	SAMEA4384070_2039

SRHZ:

FO014_03060

RAQ:	Rahaq2_5077

ROX:	BV494_15295

RBON:

QNM34_08325

DDQ:

DDI_2040

MHAN:

K6958_15770

HPAR:

AL518_09555

EAK:	EKH55_4419

STEG:

QA637_22130

ATF:	Ach5_48470

RET:	RHE_PE00355

REL:	REMIM1_PD00373

REP:	IE4803_PD00527

REI:	IE4771_PE00542

RLT:	Rleg2_5748

RLG:

Rleg_4711

RLU:	RLEG12_01740

RHL:	LPU83_pLPU83c0042

RHN:	AMJ98_PD00372

RPHA:

AMC79_PA00132

RHX:	AMK02_PE00373

RHK:	Kim5_PD00500

REZ:	AMJ99_PC00373

RII:	FFM53_024865

RHID:

FFM81_030170

RBQ:	J2J99_26115

RLN:	J0663_28945

RBAN:

J2J98_25555

RRG:	J3P73_25535

RSUL:

N2599_25805

BIF:	N288_04325

BCAB:

EFK13_09610

BACQ:

DOE78_03600

GRC:	GI584_09785

MSEM:

GMB29_03050

MSEE:

WCV65_03490

PMW:

B2K_10350

PWN:	QNH46_17500

LHI:	JP39_12245

LDX:	LH506_03595

LMM:

MI1_04685

EAV:	EH197_20055

CBEI:

LF65_04373

RUM:

CK1_34800

BHAN:

CGC63_02550

BLAR:

LC508_12690

BHV:	BLHYD_09270

LBW:	C3V36_09770

PPHP:

VTA80_09950

ART:

Arth_0051

ARR:	ARUE_c06600

ARH:	AHiyo8_09220

ARQ:	BWQ92_22735

ARN:	CGK93_03645

ARX:	ARZXY2_4154

ARTH:

C3B78_17440

AOD:	Q8Z05_08270

AAU:

AAur_0696

PUE:	FV140_20445

PNV:	JMY29_03080 JMY29_03120

PAYJ:

HMI59_01610 HMI59_01650

PAGO:

IRJ34_02995

PAYN:

HMI60_07315

PHRZ:

QN084_09285 QN084_09330

ARZ:	AUT26_18675

ACH:

Achl_2895

APN:	Asphe3_30430

PSUL:

AU252_08925

POK:	SMD14_09400

PSLS:

KTR40_16530

CELN:

CELD12_31070

NOQ:	LN652_00405

KSL:	OG809_19905

ASLA:

NCTC11923_01836

» show all

Reference

1 [PMID:1225919]

Authors

Tanaka T, Uchiyama T, Kobori H, Tanaka K.

Title

Enzymic hydrolysis of di-D-fructofuranose 1, 2'; 2, 3' dianhydride with Arthrobacter ureafaciens.

Journal

J Biochem 78:1201-6 (1975)
DOI:10.1093/oxfordjournals.jbchem.a131017

Reference

Authors

Neubauer A, Walter M, Buchholz K.

Title

Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124.

Journal

Biocatalysis and Biotransformation 18:443-455 (2000)

Reference

3 [PMID:16233453]

Authors

Saito K, Sumita Y, Nagasaka Y, Tomita F, Yokota A.

Title

Molecular cloning of the gene encoding the di-D-Fructofuranose 1,2':2,3' dianhydride hydrolysis enzyme (DFA IIIase) from Arthrobacter sp. H65-7.

Journal

J Biosci Bioeng 95:538-40 (2003)
DOI:10.1016/s1389-1723(03)80058-0

Sequence

[ag:BAD06469]

Reference

4 [PMID:26555784]

Authors

Yu S, Wang X, Zhang T, Stressler T, Fischer L, Jiang B, Mu W.

Title

Identification of a Novel Di-D-Fructofuranose 1,2':2,3' Dianhydride (DFA III) Hydrolysis Enzyme from Arthrobacter aurescens SK8.001.

Journal

PLoS One 10:e0142640 (2015)
DOI:10.1371/journal.pone.0142640

Reference

Authors

Yu S, Shen H, Cheng Y, Zhu Y, Li X, Mu W.

Title

Structural and functional basis of difructose anhydride III hydrolase, which sequentially converts inulin using the same catalytic residue.

Journal

ACS Catalysis 8:10683-10697 (2018)

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.178

IUBMB Enzyme Nomenclature:

4.2.1.178

ExPASy - ENZYME nomenclature database:

4.2.1.178

BRENDA, the Enzyme Database:

4.2.1.178

LinkDB

DBGET integrated database retrieval system