KEGG   ENZYME: 4.2.3.27
Entry
EC 4.2.3.27                 Enzyme                                 
Name
isoprene synthase;
ISPC;
ISPS;
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
prenyl-diphosphate diphosphate-lyase (isoprene-forming)
Reaction(IUBMB)
prenyl diphosphate = isoprene + diphosphate [RN:R08199]
Reaction(KEGG)
R08199
Substrate
prenyl diphosphate [CPD:C00235]
Product
isoprene [CPD:C16521];
diphosphate [CPD:C00013]
Comment
Requires Mg2+ or Mn2+ for activity. This enzyme is located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg2+ concentration [2,8].
History
EC 4.2.3.27 created 2007
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12742  isoprene synthase
Genes
ALY: 9306207
CRB: 17880730
CSAT: 104718686 104723429 104731915
BRP: 103869246
BNA: 106437444
BOE: 106301302
RSZ: 108835531
THJ: 104809623 104813173 104814037 104817852
CIT: 102626262
PVY: 116123255 116128463
MINC: 123201668 123222461 123222493 123223710 123223711 123223712
GRA: 105780734 105780735
GHI: 107898356 107948996
GAB: 108459891
HSYR: 120137220 120142667 120150919 120150921
DZI: 111281612 111282169
EGR: 104424788 104424789 104424822 104427170 104427201 104427202 104427205 104430709 120289311 120289653 120290023
RARG: 115750911
GMX: 100782452(TPS3) 100786684(TPS23) 100801629(TPS8) 100814880(TPS9)
GSJ: 114367474 114378664 114402973 114417283
PVU: 137832616 137832804 137833128 137834411 137834530 137835711 137835729 137835730
VRA: 106758286 106758670 106758896 106771573 106780025 106780479
VAR: 108332923 108339360 108339363 128196718
VUN: 114168156 114168157 114170371 114184762 114184763 114190704
VUM: 124822890 124831682 124833701
CCAJ: 109788984 109793359 109795221 109800348 109812765 109817029 109817034
APRC: 113849434 113859711 113859712 113870923
TPRA: 123881736
PSAT: 127104156 127107065 127107066
VVO: 131601729 131606711
LJA: 130746999
ADU: 107461928 107462094 107467723 107472126 107477816 127744051
AIP: 107614308 107614309 107619403 107629942
AHF: 112707039 112707040 112712397 112737563 112772760 112772761 112779715 112789658
LANG: 109355202
PCIN: 129297098 129312385 129313230
QSA: O6P43_000625 O6P43_014061 O6P43_028726
FVE: 101297281 101305692 101310344
AANS: 126798920 126798921 126798923
RCN: 112170738 112187983 112191500
MDM: 114822748
MSYL: 126611736 126612183
PXB: 103946958 103946959
PCOX: 137709184
ZJU: 107409508 107416905 107418472
MNT: 21401277 21401279
RCU: 8260996
MEAN: 126680010
JCU: 105631119 105631122 105644031 105648248
HBR: 110634890 110651136
MESC: 110627516 110627517 110627952
POP: 18100875 18107022 7485020
PEU: 105110039 105112403 105112405 105119775
PALZ: 118040300 118040301 118054959 118054961
PNZ: 133671290 133672401
JRE: 108979116 108991961 108991983
CILL: 122303857 122303892
CAVE: 132181657 132181663 132182366 132182685 132182717 132182729 132182934
AGLU: 133860209 133860213 133861343 133861344 133861345 133861346 133861381
QSU: 111989249
QLO: 115955012 115955086 115956777 115957700 115981122
TWL: 119992103 120007178
VVI: 100243167 100263737 100265475
VRI: 117926341 117926354 117926476
INI: 109163391 109163393 109163396 109163416 109188031
ITR: 116010101 116010102 116032902 116033532
SIND: 105165226
OEU: 111368363 111374106 111383800 111408326
SSPL: 121773664 121773702 121773788 121773820 121773925
APAN: 127247760
HAN: 110874622 110876334 110878487 110878488
ECAD: 122586432 122587667 122595421 122600593
LSV: 111881302 111917018 111917020
DCR: 108192517
CSIN: 114259935 114281498 114295355
RVL: 131323085 131323086 131323087 131323088 131323090 131323628 131323633
AEW: 130791847
DLT: 127791527 127791672 127791673 127792222 127792370 127792527
BVG: 104883790 104890251
SOE: 110781021 110787756
CQI: 110698900 110703332
ATRI: 130810958 130817596
MOF: 131148670
PSOM: 113320214 113323354 113325331 113344198 113344201 113358522
BDI: 100839060 100839782 104583049
ATS: 109735704 109759065 109777110
TDC: 119333939 119358627 119360217 119366829
SBI: 8081467
PVIR: 120691740
PDA: 103698991 103699093 103699243 103704724
EGU: 105046866 105057539
DCT: 110097122 110100458 110100459
PEQ: 110032081
AOF: 109828583 109830761 109833317 109833319
MSIN: 131220240 131237670
 » show all
Reference
1  [PMID:16668590]
  Authors
Silver GM, Fall R.
  Title
Enzymatic Synthesis of Isoprene from Dimethylallyl Diphosphate in Aspen Leaf Extracts.
  Journal
Plant Physiol 97:1588-1591 (1991)
DOI:10.1104/pp.97.4.1588
Reference
2  [PMID:7768893]
  Authors
Silver GM, Fall R.
  Title
Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere.
  Journal
J Biol Chem 270:13010-6 (1995)
DOI:10.1074/jbc.270.22.13010
Reference
3  [PMID:12226383]
  Authors
Wildermuth MC, Fall R.
  Title
Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts).
  Journal
Plant Physiol 112:171-182 (1996)
DOI:10.1104/pp.112.1.171
Reference
4
  Authors
Schnitzler JP, Arenz R, Steinbrecher R, Lehming A.
  Title
Characterization of an isoprene synthase from leaves of Quercus petraea.
  Journal
Bot Acta 109:216-221 (1996)
Reference
5  [PMID:11506373]
  Authors
Miller B, Oschinski C, Zimmer W
  Title
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli.
  Journal
Planta 213:483-7 (2001)
DOI:10.1007/s004250100557
  Sequence
[ag:CAC35696]
Reference
6  [PMID:12054742]
  Authors
Sivy TL, Shirk MC, Fall R.
  Title
Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis.
  Journal
Biochem Biophys Res Commun 294:71-5 (2002)
DOI:10.1016/S0006-291X(02)00435-7
Reference
7  [PMID:15848197]
  Authors
Sasaki K, Ohara K, Yazaki K
  Title
Gene expression and characterization of isoprene synthase from Populus alba.
  Journal
FEBS Lett 579:2514-8 (2005)
DOI:10.1016/j.febslet.2005.03.066
  Sequence
[ag:BAD98243]
Reference
8  [PMID:16052321]
  Authors
Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ.
  Title
Biochemical properties of isoprene synthase in poplar (Populus x canescens).
  Journal
Planta 222:777-86 (2005)
DOI:10.1007/s00425-005-0022-1
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.27
IUBMB Enzyme Nomenclature: 4.2.3.27
ExPASy - ENZYME nomenclature database: 4.2.3.27
BRENDA, the Enzyme Database: 4.2.3.27
CAS: 139172-14-8
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