KEGG   ENZYME: 5.4.3.3
Entry
EC 5.4.3.3                  Enzyme                                 
Name
lysine 5,6-aminomutase;
beta-lysine 5,6-aminomutase;
beta-lysine mutase;
L-beta-lysine 5,6-aminomutase;
D-lysine 5,6-aminomutase;
D-alpha-lysine mutase;
adenosylcobalamin-dependent D-lysine 5,6-aminomutase
Class
Isomerases;
Intramolecular transferases;
Transferring amino groups
Sysname
(3S)-3,6-diaminohexanoate 5,6-aminomutase
Reaction(IUBMB)
(1) (3S)-3,6-diaminohexanoate = (3S,5S)-3,5-diaminohexanoate [RN:R03275];
(2) D-lysine = (2R,5S)-2,5-diaminohexanoate [RN:R02852]
Reaction(KEGG)
R02852 R03275
Substrate
(3S)-3,6-diaminohexanoate [CPD:C01142];
D-lysine [CPD:C00739]
Product
(3S,5S)-3,5-diaminohexanoate [CPD:C01186];
(2R,5S)-2,5-diaminohexanoate [CPD:C05161]
Comment
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. It requires pyridoxal 5'-phosphate and adenosylcobalamin for activity. A 5'-deoxyadenosyl radical is generated during the reaction cycle by reductive cleavage of adenosylcobalamin, which is regenerated at the end of the reaction.
History
EC 5.4.3.3 created 1972 (EC 5.4.3.4 created 1972, incorporated 2017), modified 2017
Pathway
ec00310  Lysine degradation
ec00470  D-Amino acid metabolism
ec01100  Metabolic pathways
Orthology
K01844  beta-lysine 5,6-aminomutase alpha subunit
K18011  beta-lysine 5,6-aminomutase beta subunit
Genes
RFR: Rfer_3887 Rfer_3888
NOK: FAY22_04155 FAY22_04160
ARES: IWH25_08670 IWH25_08675
APET: ToN1_41370 ToN1_41380
AZA: AZKH_1576 AZKH_1577
AZI: AzCIB_1068 AzCIB_1069
DEK: DSLASN_49400 DSLASN_49410
ADE: Adeh_1483 Adeh_1485
ACP: A2cp1_2470 A2cp1_2472
AFW: Anae109_2347 Anae109_2349
ANK: AnaeK_2382 AnaeK_2384
AORY: AMOR_53280 AMOR_53300
APAU: AMPC_09780 AMPC_09800
MXA: MXAN_4386(kamE) MXAN_4388(kamD)
MSD: MYSTI_04875 MYSTI_04877
MYM: A176_002515 A176_002517
MFB: MFUL124B02_26680 MFUL124B02_26690
CCX: COCOR_03525(kamD) COCOR_03527(kamE)
MFU: LILAB_30020 LILAB_30030
MMAS: MYMAC_004300 MYMAC_004302
SUR: STAUR_4746(kamE) STAUR_4748(kamD)
AGE: AA314_04546 AA314_04548
AVM: JQX13_36710 JQX13_36720
MBD: MEBOL_007833 MEBOL_007835
VIN: AKJ08_2038 AKJ08_2039
SCL: sce2889(kamD) sce2891(kamE)
SCU: SCE1572_17625 SCE1572_17630
CCRO: CMC5_058650 CMC5_058660
PAUU: E8A73_024820 E8A73_024825
MRM: A7982_03845 A7982_03847
LLU: AKJ09_09143 AKJ09_09144
BSED: DN745_06625 DN745_14905
PCAY: FRD00_11200 FRD00_21455
BBAE: FRD01_07905 FRD01_18255
AFX: JZ786_00820 JZ786_00825
ACUR: JZ785_19365 JZ785_19370
ACYC: JI721_05870 JI721_05875
TUM: CBW65_05610 CBW65_05615
TAB: CIG75_01555 CIG75_01560
LFB: C1X05_16395 C1X05_16400
TVU: AB849_005135 AB849_005140
KPUL: GXN76_15745 GXN76_15750
KEB: GXN75_17080 GXN75_17085
PABS: JIR001_31210 JIR001_31220
CLS: CXIVA_06620 CXIVA_06630
CPAT: CLPA_c31440(kamE) CLPA_c31450(kamD)
CPAE: CPAST_c31440(kamE) CPAST_c31450(kamD)
CEU: A7L45_04310 A7L45_04315
CARG: RSJ17_19885 RSJ17_19890
CCAA: KQH81_17185 KQH81_17190
CRW: CROST_000220(kamD) CROST_000230(kamE)
CFB: CLFE_002770(kamE) CLFE_002780(kamD)
CAUN: CLAUR_029720(kamE) CLAUR_029730(kamD)
CTAG: LL095_01380 LL095_01385
AMT: Amet_4548 Amet_4549
AOE: Clos_0421 Clos_0422
GFE: Gferi_11510 Gferi_11515
CRS: FQB35_13830
CPRF: K7H06_00045 K7H06_00050
CRAS: KVH43_03350 KVH43_03355
OCW: OW730_07290 OW730_07295
FPLA: A4U99_02910 A4U99_02915
VFA: MM35RIKEN_07320 MM35RIKEN_07330
VCOP: MM50RIKEN_12190 MM50RIKEN_12200
LUA: D4A81_03630 D4A81_03635
CST: CLOST_1378(kamE) CLOST_1379(kamD)
TEB: T8CH_2398(kamE) T8CH_2399
STH: STH37 STH38
DAI: Desaci_1866 Desaci_1867
FWA: DCMF_21040 DCMF_21045
AACX: DEACI_2074
VGU: HYG85_18550 HYG85_18555
VPY: HZI73_17975 HZI73_17980
ACAE: HYG86_10315 HYG86_10320
IBU: IB211_00981 IB211_00982
AMIJ: EQM06_11635 EQM06_11640
AMIC: Ami3637_09155 Ami3637_09160
ABUT: Ami103574_14985 Ami103574_14990
ETM: CE91St48_07420 CE91St48_07430
EUU: ADJ67_02650 ADJ67_02655
TTE: TTE0726 TTE0727
TWI: Thewi_0855 Thewi_0856
NTH: Nther_1905 Nther_1906
HPK: Hprae_1560 Hprae_1561
HALS: D7D81_18055 D7D81_18060
IFN: GM661_14620 GM661_14625
AFT: BBF96_12065 BBF96_12070
KME: H0A61_00949 H0A61_00950(kamD)
SPOA: EQM13_02805 EQM13_02810
KPAR: JL105_00845 JL105_00850
CAZO: G3A45_10725 G3A45_10730
SEDZ: JYG23_11320 JYG23_11325
PUF: UFO1_1640 UFO1_1641
PFT: JBW_02724 JBW_02725
MANR: MPAN_015970 MPAN_015980
MBJ: KQ51_00865(kamD) KQ51_00866(kamE)
XCL: G4Z02_00805 G4Z02_00810
TBM: BK010_00755 BK010_00760
TBZ: BK011_00655 BK011_00660
TBK: HF295_06590 HF295_06595
SCX: AS200_02795
ICA: Intca_1791 Intca_1792
ARS: ADJ73_12505 ADJ73_12510
TEH: GKE56_04785 GKE56_04790
PHW: G7075_06380 G7075_06385
PEI: H9L10_06015
SERW: FY030_07855 FY030_07860
ORN: DV701_02935 DV701_02940
NCA: Noca_2663 Noca_2664
NOY: EXE57_02160 EXE57_02165
NOI: FCL41_08530 FCL41_08535
NOO: FE634_10520 FE634_10525
NDP: E2C04_09130 E2C04_09135
NSN: EXE58_17595 EXE58_17600
NANO: G5V58_11085 G5V58_11090
NMES: H9L09_20180 H9L09_20185
NPI: G7071_14780
NAQU: ENKNEFLB_02384(kamE) ENKNEFLB_02385(kamD)
NRO: K8W59_08665 K8W59_08670
NOQ: LN652_02045 LN652_02050
NCQ: K6T13_09125 K6T13_09130
NPS: KRR39_08650 KRR39_08655
KFL: Kfla_3601 Kfla_3602
KQI: F1D05_03990 F1D05_03995
TCU: Tcur_2347
AGRA: AGRA3207_000441 AGRA3207_000442
BLAP: MVA48_03880
MMAR: MODMU_2631 MODMU_2632
SESP: BN6_43170 BN6_43180
SSYI: EKG83_24030 EKG83_24035
KAL: KALB_4418 KALB_4419
KPHY: AOZ06_27985 AOZ06_27990
LED: BBK82_41115 BBK82_41120
ALO: CRK56464 CRK56465
ACTU: Actkin_03090(kamD) Actkin_03091(kamE)
STP: Strop_2265 Strop_2266
SAQ: Sare_2384 Sare_2385
MAU: Micau_3122 Micau_3123
MIL: ML5_5272 ML5_5273
MICB: MicB006_2176 MicB006_2177
MTUA: CSH63_09500 CSH63_09505
MICH: FJK98_17890 FJK98_17895
MTEM: GCE86_10830 GCE86_10835
MCAB: HXZ27_19850 HXZ27_19855
MSAG: GCM10017556_54740 GCM10017556_54750
VMA: VAB18032_20380 VAB18032_20385
VER: HUT12_17880 HUT12_17885
MENO: Jiend_59930
ASE: ACPL_2802(kamE) ACPL_2803
AMS: AMIS_47590 AMIS_47600
ACTN: L083_3927 L083_3928(kamE)
AFS: AFR_21805 AFR_21810
ACTS: ACWT_2743 ACWT_2744
AIH: Aiant_75460 Aiant_75470
ACTR: Asp14428_06240 Asp14428_06250
PLK: CIK06_14140 CIK06_14145
PLAB: C6361_03355
PLAT: C6W10_18270
PFLA: Pflav_024670 Pflav_024700
PSUU: Psuf_079150 Psuf_079160
ATL: Athai_29170 Athai_29180
ASER: Asera_45360 Asera_45370
PRY: Prubr_60570
CATI: CS0771_51100 CS0771_51110
DVC: Dvina_27770 Dvina_27775
DFU: Dfulv_24925 Dfulv_24930
DROS: Drose_19990 Drose_19995
DAUR: Daura_26275 Daura_26280
DMAT: Dmats_23095 Dmats_23100
NHY: JQS43_13150 JQS43_13155
CCAZ: COUCH_20865 COUCH_20870
SNA: Snas_3949 Snas_3950
NAV: JQS30_07340 JQS30_07345
GLY: K3N28_19455 K3N28_19460
FNU: FN1862 FN1863
FNC: HMPREF0946_01610 HMPREF0946_01611
FNT: HMPREF0405_01367 HMPREF0405_01368
FUS: HMPREF0409_01390 HMPREF0409_01391
FNE: FSDG_01978 FSDG_01979
FHW: RN87_00375 RN87_00380
FPD: CTM68_06750 CTM68_06755
FVA: FV113G1_13810 FV113G1_13820
FUL: C4N20_15435 C4N20_15440
FGO: C4N16_05160 C4N16_05165
FNF: BSQ88_03875 BSQ88_03880
FPEI: C4N17_12385 C4N17_12390
FCI: I6I83_03030 I6I83_03035
FHO: H9Q81_09925 H9Q81_09930
CSOM: MKD34_03805 MKD34_03810
PGI: PG_1073(kamD) PG_1074(kamE)
PGN: PGN_1169 PGN_1170
PGT: PGTDC60_1134(kamE) PGTDC60_1135(kamD)
PAH: Poras_0579 Poras_0580
PCRE: NCTC12858_01320 NCTC12858_01321
PCAG: NCTC12856_01008 NCTC12856_01009
PEND: LA319_06765 LA319_06770
PSOE: CE91St14_15540 CE91St14_15550
PET: PEIBARAKI_4145 PEIBARAKI_4146
OSP: Odosp_3176 Odosp_3177
BUY: D8S85_10245 D8S85_10250
BVIR: I6J59_05580 I6J59_05585
BACC: BRDCF_p1067 BRDCF_p1068
MBAS: ALGA_2260 ALGA_2261
CACI: CLOAM1503(kamE) CLOAM1504(kamD)
TLE: Tlet_1712 Tlet_1713
PHY: AJ81_06585 AJ81_06590
TME: Tmel_0196 Tmel_0197
TAF: THA_428 THA_429
THP: BG95_02115 BG95_02120
THER: Y592_02120 Y592_02125
TFE: JYK00_06010 JYK00_06015
FNO: Fnod_0202 Fnod_0203
FPE: Ferpe_0545 Ferpe_0546
FIA: NA23_03040 NA23_03045
FRR: IB67_05740 IB67_05745
OCY: OSSY52_05610 OSSY52_05620
PMO: Pmob_1084 Pmob_1085
MPZ: Marpi_0482 Marpi_0483
MARN: LN42_02785 LN42_02790
OCS: OF820_10920 OF820_10925
KOL: Kole_0958 Kole_0959
KPF: IX53_04790 IX53_04795
MPG: Theba_2106 Theba_2107
MINF: MESINF_1640(kamD) MESINF_1641
ASAC: ATHSA_1213 ATHSA_1214
PSYT: DSAG12_00552(mtbC_2) DSAG12_00553
 » show all
Reference
1  [PMID:4229021]
  Authors
Stadtman TC, Tsai L.
  Title
A cobamide coenzyme dependent migration of the epsilon-amino group of D-lysine.
  Journal
Biochem Biophys Res Commun 28:920-6 (1967)
DOI:10.1016/0006-291X(67)90067-8
Reference
2  [PMID:5649516]
  Authors
Stadtman TC, Renz P.
  Title
Anaerobic degradation of lysine. V. Some properties of the cobamide coenzyme-dependent beta-lysine mutase of Clostridium sticklandii.
  Journal
Arch Biochem Biophys 125:226-39 (1968)
DOI:10.1016/0003-9861(68)90657-7
Reference
3  [PMID:5480154]
  Authors
Morley CG, Stadtman TC.
  Title
Studies on the fermentation of D-alpha-lysine. Purification and properties of an adenosine triphosphate regulated B 12-coenzyme-dependent D-alpha-lysine mutase complex from Clostridium sticklandii.
  Journal
Biochemistry 9:4890-900 (1970)
DOI:10.1021/bi00827a010
Reference
4
  Authors
Retey, J., Kunz, F., Arigoni, D. and Stadtman, T.C.
  Title
Zur Kenntnis der beta-Lysin-Mutase-Reaktion: mechanismus und sterischer Verlauf.
  Journal
Helv Chim Acta 61:2989-2998 (1978)
Reference
5  [PMID:10617592]
  Authors
Chang CH, Frey PA
  Title
Cloning, sequencing, heterologous expression, purification, and characterization  of adenosylcobalamin-dependent D-lysine 5, 6-aminomutase from Clostridium sticklandii.
  Journal
J Biol Chem 275:106-14 (2000)
DOI:10.1074/jbc.275.1.106
  Sequence
[cst:CLOST_1379 CLOST_1378]
Reference
6  [PMID:12093296]
  Authors
Tang KH, Harms A, Frey PA
  Title
Identification of a novel pyridoxal 5'-phosphate binding site in adenosylcobalamin-dependent lysine 5,6-aminomutase from Porphyromonas gingivalis.
  Journal
Biochemistry 41:8767-76 (2002)
DOI:10.1021/bi020255k
Reference
7  [PMID:19634897]
  Authors
Tang KH, Mansoorabadi SO, Reed GH, Frey PA
  Title
Radical triplets and suicide inhibition in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase.
  Journal
Biochemistry 48:8151-60 (2009)
DOI:10.1021/bi900828f
Reference
8  [PMID:15514022]
  Authors
Berkovitch F, Behshad E, Tang KH, Enns EA, Frey PA, Drennan CL
  Title
A locking mechanism preventing radical damage in the absence of substrate, as revealed by the x-ray structure of lysine 5,6-aminomutase.
  Journal
Proc Natl Acad Sci U S A 101:15870-5 (2004)
DOI:10.1073/pnas.0407074101
  Sequence
[cst:CLOST_1379 CLOST_1378]
Other DBs
ExplorEnz - The Enzyme Database: 5.4.3.3
IUBMB Enzyme Nomenclature: 5.4.3.3
ExPASy - ENZYME nomenclature database: 5.4.3.3
BRENDA, the Enzyme Database: 5.4.3.3
CAS: 9075-69-8
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