KEGG   ENZYME: 6.1.1.23
Entry
EC 6.1.1.23                 Enzyme                                 
Name
aspartate---tRNAAsn ligase;
nondiscriminating aspartyl-tRNA synthetase
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
Sysname
L-aspartate:tRNAAsx ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-aspartate + tRNAAsx = AMP + diphosphate + L-aspartyl-tRNAAsx [RN:R03647 R05577]
Reaction(KEGG)
R03647 R05577
Substrate
ATP [CPD:C00002];
L-aspartate [CPD:C00049];
tRNAAsx
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-aspartyl-tRNAAsx
Comment
When this enzyme acts on tRNAAsp, it catalyses the same reaction as EC 6.1.1.12, aspartate---tRNA ligase. It has, however, diminished discrimination, so that it can also form aspartyl-tRNAAsn. This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon [1]. This accounts for the ability of this enzyme in, for example, Thermus thermophilus, to recognize both tRNAAsp (GUC anticodon) and tRNAAsn (GUU anticodon). The aspartyl-tRNAAsn is not used in protein synthesis until it is converted by EC 6.3.5.6, asparaginyl-tRNA synthase (glutamine-hydrolysing), into asparaginyl-tRNAAsn.
History
EC 6.1.1.23 created 2002
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K09759  nondiscriminating aspartyl-tRNA synthetase
Genes
PATLKGI96_09410
BANBA_2186(aspS1)
BARGBAA_2186(aspS1)
BATBAS2031
BAHBAMEG_2410(aspS1)
BAIBAA_2247(aspS1)
BAXH9401_2072
BANTA16_22180
BANRA16R_22450
BANSBAPAT_2088
BANHHYU01_10910
BANVDJ46_983(aspS)
BCEBC2169
BCZBCE33L1981(aspS)
BCRBCAH187_A2327(aspS)
BCBBCB4264_A2191(aspS)
BCUBCAH820_2205(aspS1)
BCGBCG9842_B3128(aspS1)
BCQBCQ_2153(aspS-1)
BCXBCA_2271(aspS1)
BALBACI_c21390(aspRS)
BNCBCN_2142
BCFbcf_10950
BCERBCK_23745(aspC)
BTKBT9727_1999(aspS)
BTLBALH_1944(aspS)
BTBBMB171_C1950(aspC)
BTTHD73_2454
BTHIBTK_12170(aspC)
BTCCT43_CH2117(aspC)
BTFYBT020_11265(aspC)
BTMMC28_1393
BTGBTB_c22300(aspS1)
BTIBTG_09045(aspC)
BTNBTF1_08350(aspC)
BTHTH175_ch2148
BTHUYBT1518_11975(aspC)
BTWBF38_3393(aspS)
BTHYAQ980_18775
BWEBcerKBAB4_2021
BWWbwei_2838(aspS2)
BMYOBG05_3817(aspS)
BTYBtoyo_4802
BMYCDJ92_4774(aspS)
BBYCY96_09975
BWDCT694_11700
BTROFJR70_08695(aspS)
BMOBMLA2C4_11020
BNTGSN03_10625(aspS)
BPACLMD38_15665(aspS)
BCIRC2I06_20390
BKOCKF48_04255
BOUI5818_01185(aspS)
LFBC1X05_00220
CACCA_C2979(aspC) CA_C3564(aspC)
CAESMB_G3015(aspS) SMB_G3605(aspC)
CAYCEA_G2986(aspC1) CEA_G3571(aspC3)
CPFCPF_0770(aspS)
CBOCBO1019(aspS)
CBACLB_1059(aspS-1)
CBHCLC_1072(aspC)
CBYCLM_1174(aspS)
CBLCLK_0462(aspS)
CBKCLL_A2506(aspS)
CBBCLD_3547(aspS)
CBICLJ_B1064(aspS_1)
CBFCLI_1101(aspS-1)
CBMCBF_1071
CBJH04402_01093
CBECbei_2126
CBZCbs_2126(aspC)
CBEILF65_03514
CCBClocel_2015
CSRCspa_c31980(aspS2) Cspa_c35300(aspS3)
CPASClopa_0265 Clopa_0516
CPATCLPA_c01800(aspS1)
CPAECPAST_c01800(aspS1)
CLTCM240_0264(aspS1)
CSQCSCA_0306
CLDCLSPO_c10210(aspS1)
CACECACET_c24180(aspS2)
CCKCcar_13025
CBUTATN24_11655
CFMBJL90_18220
CARGRSJ17_04905
CDRKB9W14_16940
CDYF3K33_10610(aspS)
CFERD4Z93_08270(aspS)
CMAHC1I91_26815
CRWCROST_009700(aspS2_1) CROST_044660(aspS2_2)
AMTAmet_3390
GFEGferi_27170
PBIFKXZ80_16235(aspS)
RHEREHE19_004725(aspS)
CTHDCDO33_00490
FPLAA4U99_13900
EHAEthha_1098
RUMCK1_18240
FPRACG447_00770
CLEClole_4239
CEWEKH84_02710(aspS)
RHORHOM_10080(aspC)
RIXRO1_38980
RIMROI_28550
CCOMI6K69_06180(aspS)
ROBCK5_31400
BYLA4V09_20165
BPROPMF13cell1_01447(aspS2)
BLABEYS05_00710(aspS)
BLIQINP51_06795(aspS)
CPYCphy_0636 Cphy_1139
LACYA4V08_09210
CSCIHDCHBGLK_01726(aspS2)
CSHClosa_3356 Closa_3667
ANRAna3638_11235(aspS) Ana3638_16400(aspS)
ACELacsn021_12000(aspC_1) acsn021_27120(aspC_3)
ACHTbsdcttw_12320(aspC_1) bsdcttw_29040(aspC_2)
RTORTO_10230
CBOLCGC65_22045
DLOK5I24_14495(aspS)
SINTK5I26_09130(aspS)
EREEUBREC_1167
ERTEUR_22320
ERAERE_19140
CSTCLOST_2424(aspC)
PSORRSJ16_11270
TEMJW646_00975(aspS)
IBUIB211_01416
LASAL9O85_03415(aspS)
BPRSCK3_27450
AFTBBF96_14500
MALANCTC10135_00561(lysS_2)
NBRO3I_017205(aspC)
NTPCRH09_23085
NOZDMB37_38820
NAHF5544_07115(aspS)
NIEKV110_19205(aspS)
ROPROP_35810
SCTSCAT_p1397
SCYSCATT_p03270
SSPBCP982_00925(aspS) CP982_00940
LXLKDY119_01805(aspC)
MIPAXH82_09815
MOYCVS54_03177(aspS2)
MRNK8F61_03270(aspS)
BCVBcav_4171
CCEUCBR64_09435
OEKFFI11_012400(aspS)
ICAIntca_0647
MIKFOE78_03315(aspS)
MICGGJV80_15765(aspS)
NCANoca_1189
NOOFE634_04285(aspS)
NANOG5V58_17045(aspS)
NROK8W59_08480(aspS)
NOQLN652_07995(aspS)
KFLKfla_5221
KQIF1D05_08995(aspS)
SROSros_1870
NOABKM31_27405
NOWGBF35_19000(aspS)
NGNLCN96_19135(aspS)
FREFranean1_1527
AMDAMED_1332(aspC)
AMNRAM_06755(aspC)
AMMAMES_1324(aspC)
AMZB737_1325(aspC)
AOIAORI_6069(aspC)
AMYCCU254_27220
AMYBBKN51_23855
AABA4R43_21110
AMYYYIM_08345(asnS)
KPHYAOZ06_36050
MAUMicau_4614
MILML5_3687
MICBMicB006_3617
MTUACSH63_01610
MICHFJK98_24435(aspS)
MTEMGCE86_17415(aspS)
MCABHXZ27_23320(aspS)
MSAGGCM10017556_08330(aspC)
VMAVAB18032_27436(aspC)
ASEACPL_1611(aspC)
AMSAMIS_13830
ACTNL083_1857(aspC)
AFSAFR_09230(aspC)
ACTSACWT_1490
AIHAiant_58670(aspC)
PLKCIK06_20975
PLABC6361_33390
PLATC6W10_27030
PFLAPflav_053870(aspC)
PSUUPsuf_013620(aspC)
VERHUT12_24940(aspS)
ATLAthai_37630(aspC)
ASERAsera_36190(aspC)
PRYPrubr_05220(aspC)
CATICS0771_75000(aspC)
DVCDvina_08465(aspS)
CCAZCOUCH_30435(aspS)
CAICaci_5912
SNASnas_4889
LFSHFV01_26740(aspS)
PBFCFX0092_A3339(aspS)
KBSEPA93_30065(aspS)
DRADR_1055(aspC)
DGEDgeo_0796
DDRDeide_10020
DMRDeima_1686
DPTDeipr_0975
DGODGo_CA1645(aspC)
DPDDeipe_3557
DSWQR90_10745
DCHSY84_02865
DABAUC44_09840
DPUSU48_04360
DEZDKM44_06430
DWUDVJ83_02845
DFCDFI_07340
DEINDAAJ005_12735(aspS)
DGADEGR_19070(aspS2)
DPHEHF33_05025(aspS)
DRDLMT64_04460(aspS)
TRATrad_2070
TTHTT_C1087
TTJTTHA1452(TTHA1452)
TTSThthe16_1463
TTLTtJL18_0595
TSCTSC_c18940(aspS2)
THCTCCBUS3UF1_16670
TOSTheos_1657
TAQTO73_1534
TPARAV541_03535
TBCA0O31_01800(aspC)
TANTKNN15_00990(aspS)
MRBMrub_2287
MREK649_11095(aspC)
MSVMesil_1884
MTAIMtai_v1c07810
OPROcepr_1230
MHDMarky_1319
PUVPUV_24770(aspS-A)
TPITREPR_3639
SSMSpirs_1039
STASTHERM_c22180
STQSpith_2263
SFCSpiaf_2850
SLRL21SP2_3338 L21SP2_3488
OCKEXM22_07275(aspS)
TAZTREAZ_1118(aspS_2)
SCDSpica_0990
BHCJFL75_08325(aspS)
SBERAAC3_TM7C01G0256
MJAMJ_1555
MFEMefer_1488
MVUMetvu_1266
MFSMFS40622_0734
MIFMetin_0975
MJHJH146_0120
MESGMLAUSG7_0101(aspS)
MESAMLASG1_1284(aspS)
MIGMetig_0560
MMPMMP1616(aspS)
MMQMmarC5_1792
MMXMmarC6_1053
MMZMmarC7_0864
MMDGYY_08935(aspC)
MMAKMMKA1_18470(aspS)
MMAOMMOS7_17290(aspS)
MMADMMJJ_12200(asnS)
MAEMaeo_0028
MVNMevan_0926
MVOMvol_1504
MOKMetok_0232
METFCFE53_00230(aspS)
MTHMTH_226
MMGMTBMA_c06750(aspS)
METCMTCT_0188
MWOMWSIV6_0638(aspS)
METEtca_00206(aspS2)
METZMETMT2_0671
METKFVF72_01175(aspS)
MTHMFZP57_03035(aspS)
MTEEMTTB_08680
MSTMsp_0135(aspS)
METBAW729_06475(aspC)
MRUmru_1014(aspS)
MSIMsm_1236
MEBAbm4_0682(aspS)
MMILsm9_0043(aspS)
MEYETL18_10495(aspC)
MOLYLM1_0558
MAROMarbSA_04470
MELMetbo_1366
MEWMSWAN_0903
METHMBMB1_1065(aspS)
MFCBRM9_1267(aspS)
MFIDSM1535_1950(aspS)
MCUBMCBB_0932(aspS)
MSUBBK009_02880
METNBK008_10600
METTCIT01_09535(aspS)
METOCIT02_02110(aspS)
MEMEHYG87_09910(aspS)
MFVMfer_0591
MKAMK0710(asnS)
AFUAF_0920
AFGAFULGI_00010060
APOArcpr_0998
AVEArcve_0223
ASTAsulf_01590
FPLFerp_1526
GACGACE_1475
GAHGAH_01056
MBAMbar_A3173
MBYMSBRM_2164
MBWMSBRW_1563
MBARMSBR2_0244
MBAKMSBR3_0195
MACMA_1684(aspS)
MMAMM_0074
MMAZMmTuc01_0083
MMJMSMAS_1255
MMACMSMAC_2606
MVCMSVAZ_2099
MEKMSKOL_2102
MLSMSLAZ_2148
METMMSMTP_1255
MEFMSWH1_2013
MEQMSWHS_3057
MSJMSSAC_1526
MSZMSSIH_1439
MSWMSSIT_1491
MTHRMSTHT_0766
MTHEMSTHC_2504
MHORMSHOH_2066
MFZAOB57_012670(aspS)
MBUMbur_0772
MMETMCMEM_1059
MELOJ7W08_03770(aspS)
MMHMmah_0849
MHAZBHR79_09285
MEVMetev_1586
MZHMzhil_1110
MPYMpsy_0046
MZIHWN40_07455(aspS)
MHZMetho_1011
MTPMthe_0623
MCJMCON_1668(aspS)
MHIMhar_0069
MHUMhun_2446
MRTJKHC33_16880(aspS)
MLAMlab_0039
MEMMemar_2151
MBGBN140_1355(aspC1)
MEMAMMAB1_1731(aspS)
MCHKMchiMG62_24640
MPIMpet_1892
MBNMboo_0105
MFOMetfor_0232
MPLMpal_0276
MPDMCP_0705(aspS)
MEZMtc_1518(aspS)
RCIRCIX42(aspS)
HALVNG_0461G(aspS)
HSLOE_1687F(aspS)
HDLHALDL1_05425(aspC) HALDL1_06470
HHBHhub_1409(aspS)
HABOJRZ79_01705(aspS)
HNOLT974_02070(aspS)
HLULT972_11560(aspS)
HANRLJ422_01925(aspS)
HJEHacjB3_13010(aspC)
HALHHTSR_1476(aspC6)
HHSRHSR6_1548(aspS)
HSUHLASF_0582(aspS)
HSFHLASA_0578(aspS)
HALREFA46_005880(aspS)
HARAAArcS_2635(asnS)
HAADMW046_08625(aspS)
HALXM0R89_06395(aspS)
HMArrnAC1657(aspS)
HHIHAH_2198(aspS)
HHNHISP_11200(aspC)
HABSG26_03570(aspC)
HTABVU17_10715
HALJG9465_07135(aspS)
HSINKDQ40_02400(aspS)
NPHNP_1168A(aspS)
NMONmlp_3492(aspS)
NHOHWV23_12100(aspS)
NSALHWV07_06100(aspS)
HUTHuta_2727
HTIHTIA_2475
HALAHrd1104_02075(aspS)
HMUHmuk_3044
HALZE5139_02485(aspS)
HALLLC1Hm_2368(asnS)
HAZPGBQ70_02485(aspS)
HALIBV210_05525
HSNDV733_08940
HDSHSR122_0741(asnS)
HRRHZS55_08755(aspS)
HPELHZS54_09870(aspS)
HLTI7X12_05250(aspS)
HARCHARCEL1_00475
HDOMUK72_05210(aspS)
HWAHQ_1270A(aspS)
HWCHqrw_1299(aspS)
HVOHVO_0677(aspS)
HMEHFX_0640(aspS)
HGIABY42_03225(aspC)
HALEG3A49_03445(aspS)
HBOHbor_25400
HAQDU484_06810
HAJDU500_07080
HAERDU502_04745(aspS)
HRAEI982_09000(aspS)
HLMDV707_12690
HALMFCF25_10400(aspS)
HLAHlac_1657
HALBEKH57_02415(aspS)
HSSJ7656_07370(aspS)
HSAIHPS36_12730(aspS)
HAZZKI388_06785(aspS)
SRUBC2R22_04080
HAEhalTADL_1088
HALNB4589_008115(aspS)
HALGHUG10_14865(aspS)
HALUHUG12_01330(aspS)
HMPK6T50_11065(aspS)
HREK6T36_10855(aspS)
HRMK6T25_05190(aspS)
HACBHbl1158_03280(aspS)
HDFAArcSl_1450(aspS)
HAHHalar_3227
HTUHtur_2681
HDABB347_08750
HJTDVR14_14045
HSALJMJ58_15450(aspS)
HLOJ0X27_05115(aspS)
HAKZJ0X25_01615(aspS)
NMGNmag_1072(aspS)
HXAHalxa_2483
NATNJ7G_1985
NPENatpe_0949
NVRFEJ81_04890(aspS)
NPLFGF80_05955(aspS)
NAYHYG81_05990(aspS)
HALYHYG82_09045(aspS)
NGENatgr_2086
HRUHalru_0012 Halru_1156
NOUNatoc_2680
SALIL593_04135(aspC)
SAIMK0C01_03140(aspS)
HLRHALLA_12915(aspC)
HLCCHINAEXTREME09790
NAJB1756_12335
NAGAArcMg_0538
NANAArc1_0551
NBGDV706_10900
NASGCU68_01190(aspS)
NACAQV86_01765
MELUMTLP_03530
MEAMMU439_00450(aspS)
TACTa0946
TVOTVG1147365(TVG1147365)
PTOPTO1214
FACFACI_IFERC01G1269
FAIFAD_0280(aspS)
CDIVCPM_0581
TARTALC_00682
MAXMMALV_09960
MERMMINT_11970(aspC)
MEARMpt1_c07320(aspS2)
MARCAR505_1150
ABIAboo_0465
ACFAciM339_0578
APEAPE_2192.1(aspS)
ACJACAM_1377(aspS)
SMRSmar_0296
SHCShell_0519
IHOIgni_1435
IISEYM_06180(aspC)
DKADKAM_0911
DFDDesfe_0883
DMUDesmu_0914
TAGTagg_0704
IAGIgag_0068
THGTCELL_0668
HBUHbut_0034
PFMPyrfu_0646
PDLPyrde_0572
STOSTK_02050(aspS)
SOHD1869_00530(aspS)
SSOSSO0173(aspS)
SOLSsol_1151
SSOASULA_1187(aspS)
SSOLSULB_1188(aspS)
SSOFSULC_1186(aspS)
SSHIJ5U23_00087
SCASSACC_00860
SAISaci_0768(aspS)
SACNSacN8_03725(aspC)
SACRSacRon12I_03710(aspC)
SACSSUSAZ_03455(aspC)
SISLS215_2127
SIAM1425_1964
SIMM1627_2042
SIDM164_1972
SIYYG5714_2086
SINYN1551_0832
SIILD85_2224
SIHSiH_1909
SIRSiRe_1837
SICSiL_1818
SULABFU36_05010
SULEGFS03_10765(aspS)
SULOGFS33_02580(aspS)
SULLEWF20_01965(aspS)
MSEMsed_0205
MCNMcup_1868
MHKDFR87_05795(aspS)
MPRUDFR88_05595
MTENGWK48_06195(aspS)
MJNMjAS7_0791
AHOAhos_0632
AMANB6F84_08140
ABRIDFR85_06665(aspS)
ASULDFR86_07175(aspS)
AAMBD1866_10900(aspS)
ACIHHS5_19680
SACDHS1genome_0852
SAZOD1868_01350(aspS)
CSTYKN1_17700
STEPIC006_1883
SAHSHS7_17380
PAIPAE0703
PISPisl_0679
PCLPcal_2119
PASPars_2350
PYRP186_2206
POGPogu_2516
TNETneu_1987
PYWPYWP30_02252
CMACmaq_0746
TTNTTX_0953(aspS)
TUZTUZN_0620
VDIVdis_2301
VMOVMUT_0615
TPETpen_0628
THBN186_04490
TCBTCARB_1925
THFMA03_05930
THELIG193_03930(aspS)
ASCASAC_0458
ACIASE86_03165
CLGCalag_1552
FFOFFONT_0837
NMRNmar_0871 Nmar_1066
NIRNSED_04875 NSED_06225
NKRNKOR_04905 NKOR_06405
NIDNPIRD3C_1051(aspS) NPIRD3C_1327(aspS)
NINNADRNF5_0866(aspS) NADRNF5_1182(aspS)
NIWNisw_00385(aspS) Nisw_08115(aspS)
NCLC5F47_03150 C5F47_04405
NOXC5F49_04315 C5F49_06080
NUEC5F50_06455 C5F50_08080
NIPNsoK4_02395(aspS) NsoK4_04315(aspS)
NCTNMSP_0573(aspS_1) NMSP_0847(aspS_2)
NICDSQ20_06705
CSYCENSYa_0241 CENSYa_0611
NGANgar_c02730(aspS1) Ngar_c18880(aspS2)
NVNNVIE_003450(aspS1) NVIE_014140(aspS2)
NEVNTE_01665 NTE_02820
TAANMY3_02651(asnS_1) NMY3_03112(asnS_2)
NFNNFRAN_0257(aspS) NFRAN_0528(aspS)
NCVNCAV_0746(aspS) NCAV_1231(aspS)
CSUCSUB_C1084
NBVT478_0773(aspS) T478_0884(aspS)
TAHSU86_000905 SU86_001760
NIUDSQ19_05200 DSQ19_06215
NDVNDEV_1112(aspS) NDEV_1176(aspS)
CCAINAS2_0491 NAS2_1309
KCRKcr_1200
BARCAOA65_0635(aspS)
NEQNEQ535
NAANps_01055
MARHMia14_0202
FLTSv326_0024
MIYMicr_00748(aspS_3)
LOKILokiarch_26910(aspS)
PSYTDSAG12_02182(aspS)
ARGQT11_C0001G1016
 » show all
Reference
1  [PMID:10966471]
  Authors
Ibba M, Soll D.
  Title
Aminoacyl-tRNA synthesis.
  Journal
Annu Rev Biochem 69:617-50 (2000)
DOI:10.1146/annurev.biochem.69.1.617
Reference
2  [PMID:9724658]
  Authors
Schmitt E, Moulinier L, Fujiwara S, Imanaka T, Thierry JC, Moras D.
  Title
Crystal structure of aspartyl-tRNA synthetase from Pyrococcus kodakaraensis KOD: archaeon specificity and catalytic mechanism of adenylate formation.
  Journal
EMBO J 17:5227-37 (1998)
DOI:10.1093/emboj/17.17.5227
Reference
3  [PMID:9789000]
  Authors
Becker HD, Kern D.
  Title
Thermus thermophilus: a link in evolution of the tRNA-dependent amino acid amidation pathways.
  Journal
Proc Natl Acad Sci U S A 95:12832-7 (1998)
DOI:10.1073/pnas.95.22.12832
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.23
IUBMB Enzyme Nomenclature: 6.1.1.23
ExPASy - ENZYME nomenclature database: 6.1.1.23
BRENDA, the Enzyme Database: 6.1.1.23
CAS: 9027-32-1
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