Entry
Name
tRNAmet cytidine acetate ligase;
tmcAL (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy
Sysname
elongator tRNAmet cytidine:acetate ligase (AMP-forming)
Reaction(IUBMB)
ATP + [elongator tRNAMet]-cytidine34 + acetate = AMP + diphosphate + [elongator tRNAMet]-N4-acetylcytidine34 (overall reaction);
(1a) ATP + acetate = acetyladenylate + diphosphate;
(1b) acetyladenylate + [elongator tRNAMet]-cytidine34 = AMP + [elongator tRNAMet]-N4-acetylcytidine34
Substrate
ATP [CPD:
C00002 ];
[elongator tRNAMet]-cytidine34;
acetate [CPD:
C00033 ];
acetyladenylate
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
[elongator tRNAMet]-N4-acetylcytidine34;
acetyladenylate
Comment
The enzyme, charactrized from the bacterium Bacillus subtilis, catalyses a similar tRNA modification to that performed by EC
2.3.1.193 , tRNAMet cytidine acetyltransferase. However, unlike that enzyme, which uses acetyl-CoA as the acetyl donor, this enzyme activates an acetate ion to form acetyladenylate and then catalyses the acetylation through a mechanism similar to tRNA aminoacylation.
History
EC 6.3.1.22 created 2024
Orthology
K27343 tRNAmet cytidine acetate ligase
Genes
BAZ : BAMTA208_09620(ylbM)
PARG : PspKH34_27160(ylbM)
COHN : KCTCHS21_38870(ylbM)
LPW : LpgJCM5343_13980(ylbM)
CAUN : CLAUR_007830(tmcAL)
SSPH : SPSPH_016690(tmcAL)
SSID : SPSIL_022060(tmcAL)
SACV : SPACI_025520(tmcAL)
FIT : Fi14EGH31_07230(ylbM)
MPU : MYPU_3090(MYPU_3090)
» show all
Taxonomy
Reference
Authors
Taniguchi T, Miyauchi K, Sakaguchi Y, Yamashita S, Soma A, Tomita K, Suzuki T
Title
Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.22
ExPASy - ENZYME nomenclature database: 6.3.1.22
LinkDB
All DBs