KEGG ORTHOLOGY: K07543

ORTHOLOGY: K07543

Entry

K07543                      KO

Symbol

bbsE

Name

benzylsuccinate CoA-transferase BbsE subunit [EC:2.8.3.15]

Pathway

map00623

Toluene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00418

Toluene degradation, anaerobic, toluene => benzoyl-CoA

Reaction

R05588

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07543  bbsE; benzylsuccinate CoA-transferase BbsE subunit
Enzymes [BR:ko01000]
2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.3  CoA-transferases
    2.8.3.15  succinyl-CoA:(R)-benzylsuccinate CoA-transferase
     K07543  bbsE; benzylsuccinate CoA-transferase BbsE subunit

Other DBs

GO:

0018728

Genes

BYI:	BYI23_C006090(bbsE)

BUE:	BRPE67_CCDS05370(bbsE)

BCAI:

K788_0001721

BBR:	BB4333(bbsE)

BBX:	BBS798_4110(bbsE)

BFZ:	BAU07_03770

BGM:	CAL15_20530

AXY:	AXYL_00933

ADY:	HLG70_07305

APES:

FOC84_07710 FOC84_19020

BOZ:	DBV39_02105

EBA:	c2A312(bbsE)

APET:

ToN1_01570(bbsE)

AZI:	AzCIB_4522

AZD:	CDA09_07440 CDA09_21975

TCL:

Tchl_1389

TAK:	Tharo_3086

CHEA:

PVE73_27595

HDH:	G5B40_14180

GME:	Gmet_1521(bbsE)

GEO:	Geob_2420(bbsE)

DTO:	TOL2_C01510(bbsE) TOL2_C04940(bhsE)

DMM:	dnm_089790(bhsE)

SCAD:

DN051_35615

CHRE:

IE160_04115

LDN:	H9L06_08060

BBRG:

U6N30_32530

AMQ:	AMETH_4629

DACT:

KZZ52_32780

AG:	AAF89840(bbsE)

» show all

Reference

PMID:10629170

Authors

Leuthner B, Heider J

Title

Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.

Journal

J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000

Sequence

[ag:AAF89840]

LinkDB

ORTHOLOGY: K07544

Entry

K07544                      KO

Symbol

bbsF

Name

benzylsuccinate CoA-transferase BbsF subunit [EC:2.8.3.15]

Pathway

map00623

Toluene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00418

Toluene degradation, anaerobic, toluene => benzoyl-CoA

Reaction

R05588

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07544  bbsF; benzylsuccinate CoA-transferase BbsF subunit
Enzymes [BR:ko01000]
2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.3  CoA-transferases
    2.8.3.15  succinyl-CoA:(R)-benzylsuccinate CoA-transferase
     K07544  bbsF; benzylsuccinate CoA-transferase BbsF subunit

Other DBs

GO:

0018728

Genes

BYI:	BYI23_C006080

BUE:	BRPE67_CCDS05380

BCAI:

K788_0001720

PPAI:

E1956_34635

BBR:	BB4334(bbsF)

BBX:	BBS798_4111(bbsF)

BFZ:	BAU07_03775

BGM:	CAL15_20535

AXY:	AXYL_00934

ADY:	HLG70_07300

APES:

FOC84_07705

BOZ:	DBV39_02110

VEI:

Veis_4269

DOE:	DENOEST_2960

EBA:	c2A311(bbsF)

APET:

ToN1_01560(bbsF)

AZI:	AzCIB_4521

AZD:	CDA09_07445 CDA09_21970

TCL:

Tchl_1390

TAK:	Tharo_3085

XDI:	EZH22_10375

STAR:

G3545_21825

MHAR:

L1P08_14745

CON:	TQ29_05735

HDH:	G5B40_14185

RDI:	CMV14_18955

SBIN:

SBA_ch2_5300

SHUM:

STHU_16110

SVC:	STVA_36930

STEL:

STAQ_12170

GME:	Gmet_1522(bbsF)

GEO:	Geob_2421(bbsF)

DTO:	TOL2_C01520(bbsF) TOL2_C04950(bhsF)

DOV:	DSCO28_65710

DWD:	DSCW_52970

DMM:	dnm_089800(bhsF)

DMP:

FAK_35180

DGI:	Desgi_0646

MSTO:

MSIM:

MGOR:

MGAU:

MDU:	MDUV_29070

MMOR:

MMOR_19340

MGAD:

MGAD_51010

MGO:	AFA91_20445

RKO:	JWS14_43335

SCAD:

DN051_35620

SVD:	CP969_04130

CHRE:

IE160_04120

LDN:	H9L06_08055

AMQ:	AMETH_4630

DACT:

KZZ52_32775

TFLA:

O0235_03140

AG:	AAF89841(bbsF)

» show all

Reference

PMID:10629170

Authors

Leuthner B, Heider J

Title

Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.

Journal

J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000

Sequence

[ag:AAF89841]

LinkDB

ENZYME: 2.8.3.15

Entry

EC 2.8.3.15                 Enzyme

Name

succinyl-CoA:(R)-benzylsuccinate CoA-transferase;
benzylsuccinate CoA-transferase

Class

Transferases;
Transferring sulfur-containing groups;
CoA-transferases

Sysname

succinyl-CoA:(R)-2-benzylsuccinate CoA-transferase

Reaction(IUBMB)

succinyl-CoA + (R)-2-benzylsuccinate = succinate + (R)-2-benzylsuccinyl-CoA [RN:R05588]

Reaction(KEGG)

R05588

Substrate

succinyl-CoA [CPD:C00091];
(R)-2-benzylsuccinate [CPD:C09816]

Product

succinate [CPD:C00042];
(R)-2-benzylsuccinyl-CoA [CPD:C09817]

Comment

Involved in anaerobic catabolism of toluene and is a strictly toluene-induced enzyme that catalyses the reversible regio- and enantio-selective synthesis of (R)-2-benzylsuccinyl-CoA. The enzyme from Thauera aromatica is inactive when (R)-benzylsuccinate is replaced by (S)-benzylsuccinate.

History

EC 2.8.3.15 created 2003

Pathway

ec00623

Toluene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K07543

benzylsuccinate CoA-transferase BbsE subunit

K07544

benzylsuccinate CoA-transferase BbsF subunit

Genes

BYI:	BYI23_C006080 BYI23_C006090(bbsE)

BUE:	BRPE67_CCDS05370(bbsE) BRPE67_CCDS05380

BCAI:

K788_0001720 K788_0001721

PPAI:

E1956_34635

BBR:	BB4333(bbsE) BB4334(bbsF)

BBX:	BBS798_4110(bbsE) BBS798_4111(bbsF)

BFZ:	BAU07_03770 BAU07_03775

BGM:	CAL15_20530 CAL15_20535

AXY:	AXYL_00933 AXYL_00934

ADY:	HLG70_07300 HLG70_07305

APES:

FOC84_07705 FOC84_07710 FOC84_19020

BOZ:	DBV39_02105 DBV39_02110

VEI:

Veis_4269

DOE:	DENOEST_2960

EBA:	c2A311(bbsF) c2A312(bbsE)

APET:

ToN1_01560(bbsF) ToN1_01570(bbsE)

AZI:	AzCIB_4521 AzCIB_4522

AZD:	CDA09_07440 CDA09_07445 CDA09_21970 CDA09_21975

TCL:	Tchl_1389 Tchl_1390

TAK:	Tharo_3085 Tharo_3086

CHEA:

PVE73_27595

XDI:	EZH22_10375

STAR:

G3545_21825

MHAR:

L1P08_14745

CON:	TQ29_05735

HDH:	G5B40_14180 G5B40_14185

RDI:	CMV14_18955

SBIN:

SBA_ch2_5300

SHUM:

STHU_16110

SVC:	STVA_36930

STEL:

STAQ_12170

GME:	Gmet_1521(bbsE) Gmet_1522(bbsF)

GEO:	Geob_2420(bbsE) Geob_2421(bbsF)

DTO:	TOL2_C01510(bbsE) TOL2_C01520(bbsF) TOL2_C04940(bhsE) TOL2_C04950(bhsF)

DOV:	DSCO28_65710

DWD:	DSCW_52970

DMM:	dnm_089790(bhsE) dnm_089800(bhsF)

DMP:

FAK_35180

DGI:	Desgi_0646

MSTO:

MSTO_21600

MSIM:

MSIM_33930

MGOR:

H0P51_08430

MGAU:

MGALJ_32240

MDU:	MDUV_29070

MMOR:

MMOR_19340

MGAD:

MGAD_51010

MGO:	AFA91_20445

RKO:	JWS14_43335

SCAD:

DN051_35615 DN051_35620

SVD:	CP969_04130

CHRE:

IE160_04115 IE160_04120

LDN:	H9L06_08055 H9L06_08060

BBRG:

U6N30_32530

AMQ:	AMETH_4629 AMETH_4630

DACT:

KZZ52_32775 KZZ52_32780

TFLA:

O0235_03140

» show all

Reference

1 [PMID:11418570]

Authors

Leutwein C, Heider J

Title

Succinyl-CoA:(R)-benzylsuccinate CoA-transferase: an enzyme of the anaerobic toluene catabolic pathway in denitrifying bacteria.

Journal

J Bacteriol 183:4288-95 (2001)
DOI:10.1128/JB.183.14.4288-4295.2001

Sequence

[ag:AAF89840 AAF89841]

Reference

2 [PMID:10589736]

Authors

Leutwein C, Heider J.

Title

Anaerobic toluene-catabolic pathway in denitrifying Thauera aromatica: activation and beta-oxidation of the first intermediate, (R)-(+)-benzylsuccinate.

Journal

Microbiology 145 ( Pt 11):3265-71 (1999)
DOI:10.1099/00221287-145-11-3265

Reference

3 [PMID:10629170]

Authors

Leuthner B, Heider J

Title

Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.

Journal

J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000

Sequence

[ag:AAF89840 AAF89841]

Reference

4 [PMID:11749953]

Authors

Heider J.

Title

A new family of CoA-transferases.

Journal

FEBS Lett 509:345-9 (2001)
DOI:10.1016/S0014-5793(01)03178-7

Other DBs

ExplorEnz - The Enzyme Database:

2.8.3.15

IUBMB Enzyme Nomenclature:

2.8.3.15

ExPASy - ENZYME nomenclature database:

2.8.3.15

UM-BBD (Biocatalysis/Biodegradation Database):

2.8.3.15

BRENDA, the Enzyme Database:

2.8.3.15

CAS:	260966-56-1

LinkDB

REACTION: R05588

Entry

R05588                      Reaction

Definition

Benzylsuccinate + Succinyl-CoA <=> Benzylsuccinyl-CoA + Succinate

Equation

C09816 + C00091 <=> C09817 + C00042

Comment

BbsE,BbsF

Reaction class

RC00014

C00042_C00091

RC00137

C09816_C09817

Enzyme

2.8.3.15

Pathway

rn00623

Toluene degradation

rn01100

Metabolic pathways

rn01120

Microbial metabolism in diverse environments

rn01220

Degradation of aromatic compounds

Module

M00418

Toluene degradation, anaerobic, toluene => benzoyl-CoA

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.8  Transferring sulfur-containing groups
   2.8.3  CoA-transferases
    2.8.3.15
     R05588  Benzylsuccinate + Succinyl-CoA <=> Benzylsuccinyl-CoA + Succinate

Orthology

K07543

benzylsuccinate CoA-transferase BbsE subunit [EC:2.8.3.15]

K07544

benzylsuccinate CoA-transferase BbsF subunit [EC:2.8.3.15]

Other DBs

RHEA:

16472

LinkDB

DBGET integrated database retrieval system