KEGG   ORTHOLOGY: K07543
Entry
K07543                      KO                                     
Symbol
bbsE
Name
benzylsuccinate CoA-transferase BbsE subunit [EC:2.8.3.15]
Pathway
map00623  Toluene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07543  bbsE; benzylsuccinate CoA-transferase BbsE subunit
Enzymes [BR:ko01000]
 2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.3  CoA-transferases
    2.8.3.15  succinyl-CoA:(R)-benzylsuccinate CoA-transferase
     K07543  bbsE; benzylsuccinate CoA-transferase BbsE subunit
Other DBs
RN: R05588
GO: 0018728
Genes
BYI: BYI23_C006090(bbsE)
BUE: BRPE67_CCDS05370(bbsE)
BCAI: K788_0001721
BBR: BB4333(bbsE)
BBX: BBS798_4110(bbsE)
BFZ: BAU07_03770
BGM: CAL15_20530
AXY: AXYL_00933
ADY: HLG70_07305
BOZ: DBV39_02105
EBA: c2A312(bbsE)
APET: ToN1_01570(bbsE)
TCL: Tchl_1389
GME: Gmet_1521(bbsE)
GEO: Geob_2420(bbsE)
DTO: TOL2_C01510(bbsE) TOL2_C04940(bhsE)
DMM: dnm_089790(bhsE)
AG: AAF89840(bbsE)
 » show all
Reference
  Authors
Leuthner B, Heider J
  Title
Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.
  Journal
J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000
  Sequence
LinkDB

KEGG   ORTHOLOGY: K07544
Entry
K07544                      KO                                     
Symbol
bbsF
Name
benzylsuccinate CoA-transferase BbsF subunit [EC:2.8.3.15]
Pathway
map00623  Toluene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07544  bbsF; benzylsuccinate CoA-transferase BbsF subunit
Enzymes [BR:ko01000]
 2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.3  CoA-transferases
    2.8.3.15  succinyl-CoA:(R)-benzylsuccinate CoA-transferase
     K07544  bbsF; benzylsuccinate CoA-transferase BbsF subunit
Other DBs
RN: R05588
GO: 0018728
Genes
BYI: BYI23_C006080
BUE: BRPE67_CCDS05380
BCAI: K788_0001720
BBR: BB4334(bbsF)
BBX: BBS798_4111(bbsF)
BFZ: BAU07_03775
BGM: CAL15_20535
AXY: AXYL_00934
ADY: HLG70_07300
BOZ: DBV39_02110
VEI: Veis_4269
EBA: c2A311(bbsF)
APET: ToN1_01560(bbsF)
TCL: Tchl_1390
GME: Gmet_1522(bbsF)
GEO: Geob_2421(bbsF)
DTO: TOL2_C01520(bbsF) TOL2_C04950(bhsF)
DMM: dnm_089800(bhsF)
STEL: STAQ_12170
MSTO: MSTO_21600
MSIM: MSIM_33930
MMOR: MMOR_19340
MGAD: MGAD_51010
AG: AAF89841(bbsF)
 » show all
Reference
  Authors
Leuthner B, Heider J
  Title
Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.
  Journal
J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000
  Sequence
LinkDB

KEGG   ENZYME: 2.8.3.15
Entry
EC 2.8.3.15                 Enzyme                                 
Name
succinyl-CoA:(R)-benzylsuccinate CoA-transferase;
benzylsuccinate CoA-transferase
Class
Transferases;
Transferring sulfur-containing groups;
CoA-transferases
Sysname
succinyl-CoA:(R)-2-benzylsuccinate CoA-transferase
Reaction(IUBMB)
succinyl-CoA + (R)-2-benzylsuccinate = succinate + (R)-2-benzylsuccinyl-CoA [RN:R05588]
Reaction(KEGG)
R05588
Substrate
succinyl-CoA [CPD:C00091];
(R)-2-benzylsuccinate [CPD:C09816]
Product
succinate [CPD:C00042];
(R)-2-benzylsuccinyl-CoA [CPD:C09817]
Comment
Involved in anaerobic catabolism of toluene and is a strictly toluene-induced enzyme that catalyses the reversible regio- and enantio-selective synthesis of (R)-2-benzylsuccinyl-CoA. The enzyme from Thauera aromatica is inactive when (R)-benzylsuccinate is replaced by (S)-benzylsuccinate.
History
EC 2.8.3.15 created 2003
Pathway
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K07543  benzylsuccinate CoA-transferase BbsE subunit
K07544  benzylsuccinate CoA-transferase BbsF subunit
Genes
BYIBYI23_C006080 BYI23_C006090(bbsE)
BUEBRPE67_CCDS05370(bbsE) BRPE67_CCDS05380
BCAIK788_0001720 K788_0001721
BBRBB4333(bbsE) BB4334(bbsF)
BBXBBS798_4110(bbsE) BBS798_4111(bbsF)
BFZBAU07_03770 BAU07_03775
BGMCAL15_20530 CAL15_20535
AXYAXYL_00933 AXYL_00934
ADYHLG70_07300 HLG70_07305
BOZDBV39_02105 DBV39_02110
VEIVeis_4269
DOEDENOEST_2960
EBAc2A311(bbsF) c2A312(bbsE)
APETToN1_01560(bbsF) ToN1_01570(bbsE)
AZIAzCIB_4521 AzCIB_4522
AZDCDA09_07440 CDA09_07445 CDA09_21970 CDA09_21975
TCLTchl_1389 Tchl_1390
TAKTharo_3085 Tharo_3086
GMEGmet_1521(bbsE) Gmet_1522(bbsF)
GEOGeob_2420(bbsE) Geob_2421(bbsF)
DTOTOL2_C01510(bbsE) TOL2_C01520(bbsF) TOL2_C04940(bhsE) TOL2_C04950(bhsF)
DMMdnm_089790(bhsE) dnm_089800(bhsF)
XDIEZH22_10375
STARG3545_21825
CONTQ29_05735
HDHG5B40_14180 G5B40_14185
RDICMV14_18955
STELSTAQ_12170
DGIDesgi_0646
MGOAFA91_20445
MSTOMSTO_21600
MSIMMSIM_33930
MGORH0P51_08430
MGAUMGALJ_32240
MDUMDUV_29070
MMORMMOR_19340
MGADMGAD_51010
RKOJWS14_43335
RGOKYT97_15935
SCADDN051_35615 DN051_35620
SVDCP969_04130
LDNH9L06_08055 H9L06_08060
CHREIE160_04115 IE160_04120
AMQAMETH_4629 AMETH_4630
 » show all
Reference
1  [PMID:11418570]
  Authors
Leutwein C, Heider J
  Title
Succinyl-CoA:(R)-benzylsuccinate CoA-transferase: an enzyme of the anaerobic toluene catabolic pathway in denitrifying bacteria.
  Journal
J Bacteriol 183:4288-95 (2001)
DOI:10.1128/JB.183.14.4288-4295.2001
  Sequence
Reference
2  [PMID:10589736]
  Authors
Leutwein C, Heider J.
  Title
Anaerobic toluene-catabolic pathway in denitrifying Thauera aromatica: activation and beta-oxidation of the first intermediate, (R)-(+)-benzylsuccinate.
  Journal
Microbiology 145 ( Pt 11):3265-71 (1999)
DOI:10.1099/00221287-145-11-3265
Reference
3  [PMID:10629170]
  Authors
Leuthner B, Heider J
  Title
Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.
  Journal
J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000
  Sequence
Reference
4  [PMID:11749953]
  Authors
Heider J.
  Title
A new family of CoA-transferases.
  Journal
FEBS Lett 509:345-9 (2001)
DOI:10.1016/S0014-5793(01)03178-7
Other DBs
ExplorEnz - The Enzyme Database: 2.8.3.15
IUBMB Enzyme Nomenclature: 2.8.3.15
ExPASy - ENZYME nomenclature database: 2.8.3.15
UM-BBD (Biocatalysis/Biodegradation Database): 2.8.3.15
BRENDA, the Enzyme Database: 2.8.3.15
CAS: 260966-56-1
LinkDB

KEGG   REACTION: R05588
Entry
R05588                      Reaction                               
Definition
Benzylsuccinate + Succinyl-CoA <=> Benzylsuccinyl-CoA + Succinate
Equation
Comment
BbsE,BbsF
Reaction class
RC00014  C00042_C00091
RC00137  C09816_C09817
Enzyme
Pathway
rn00623  Toluene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Orthology
K07543  benzylsuccinate CoA-transferase BbsE subunit [EC:2.8.3.15]
K07544  benzylsuccinate CoA-transferase BbsF subunit [EC:2.8.3.15]
Other DBs
RHEA: 16472
LinkDB

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