KEGG ORTHOLOGY: K09587

ORTHOLOGY: K09587

Entry

K09587                      KO

Symbol

CYP90B, DWF4

Name

steroid 22S-hydroxylase [EC:1.14.14.178]

Pathway

map00905

Brassinosteroid biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00371

Castasterone biosynthesis, campesterol => castasterone

Reaction

R07430

6-oxocampestanol,NADPH:brassinosteroid C22alpha-hydroxylase

R07445

campesterol,NADPH---hemoprotein reductase:oxygen 22S-oxidoreductase (hydroxylating)

R07452

campest-4-en-3-one,NADPH---hemoprotein reductase:oxygen 22S-oxidoreductase (hydroxylating)

R07453

5alpha-campestan-3-one,NADPH---hemoprotein reductase:oxygen 22S-oxidoreductase (hydroxylating)

R07454

campestanol,NADPH---hemoprotein reductase:oxygen 22S-oxidoreductase (hydroxylating)

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00905 Brassinosteroid biosynthesis
    K09587  CYP90B, DWF4; steroid 22S-hydroxylase
09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K09587  CYP90B, DWF4; steroid 22S-hydroxylase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.178  steroid 22S-hydroxylase
     K09587  CYP90B, DWF4; steroid 22S-hydroxylase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, plant type
  CYP90 family
   K09587  CYP90B, DWF4; steroid 22S-hydroxylase

Genes

ATH:	AT3G50660(DWF4)

ALY:

9312109

CRB:

17885187

CSAT:

104780889 104791284

EUS:	EUTSA_v10011005mg

BRP:	103828198 103840968

BNA:	106401625 106405681 106447365 125575243

BOE:	106310362 106321383

RSZ:	108809973 108826420

THJ:	104819220 104826953

CPAP:

110822261

CIT:

102623486

CIC:	CICLE_v10025451mg

PVY:	116113019 116113135

MINC:

123199643 123199686 123214950

TCC:

18610945

GRA:

105803806

GHI:	107896149 107913065

GAB:

108471227

HSYR:

120139008 120139225 120171780 120196689

DZI:	111292390 111301072 111316237

EGR:

104419755

GMX:	100789515 100800210 100802498

GSJ:	114377375 114382291 114420632

PVU:	PHAVU_002G029700g PHAVU_003G253100g

VRA:	106765911 106778030

VAR:	108334671 108335822

VUN:	114166425 114179641

VUM:	124824410 124826501

CCAJ:

109801635 109810494

APRC:

113859349 113868805

MTR:	11405189 11408048

TPRA:

123908684 123920823

CAM:	101499755 101500152

PSAT:

127120259 127135353

VVO:	131608124 131608206 131652102

LJA:	Lj0g3v0324529.1(Lj0g3v0324529.1)

ADU:

107487855

AIP:

107642870

AHF:	112749146 112800701

LANG:

109331920 109348687

PCIN:

129310431 129321868

QSA:	O6P43_024752 O6P43_033718

FVE:

RCN:

PPER:

PMUM:

PAVI:

PDUL:

MDM:	103401164 103401541

MSYL:

126604824 126613856

PXB:	103941521 103960819

ZJU:

MNT:

CSAV:

CSV:

CMO:

BHJ:

MCHA:

CMAX:

CMOS:

CPEP:

RCU:

HBR:	110634320 110665021

MESC:

110603714

POP:	18099286 18101214

PEU:	105110409 105126568

PALZ:

118030029 118063301

JRE:	108995250 108997500

CILL:

CAVE:

QSU:

QLO:

TWL:	120000980 120014527

VVI:

100241065

VRI:

117913676

SLY:	101256342(CYP90B3)

SPEN:

SOT:

SSTN:

SDUL:

CANN:

LBB:

NTA:	107779615 107795954

NSY:

NTO:

NAU:

INI:

ITR:

SIND:

OEU:	111367619 111380525

EGT:

SSPL:

SMIL:

APAN:

HAN:

ECAD:

LSV:

CCAV:

DCR:

CSIN:

RVL:

AEW:

BVG:

SOE:

CQI:	110686172 110722522

ATRI:

130807369

MOF:

131162668

NNU:	104595706 104601879

MING:

122073840 122083658

TSS:	122658580 122661166

PSOM:

113289235 113293814

OSA:

4332134

DOSA:

Os03t0227700-01(Os03g0227700)

OBR:

102709630

OGL:

127768703

BDI:

100837605

ATS:	109742297 109786179

TDC:	119273638 119285031 119291093

TAES:

123059596 123066933 123075890 123085252 123092868 123098154

TUA:	125549501 125552395

LPER:

127296297

LRD:	124701853 124701863

SBI:

ZMA:

SITA:

SVS:

PVIR:

PHAI:

PDA:	103697967 103704238 103707598 103707601 120113249

EGU:	105055939 105058149 105060750

MUS:	103974765 103981133 103996193 103996195

ZOF:	122008713 122011363 122013395 122014363 122015895 122026256 122026454 122027838 122028682 122030413 122030415 122032511 122034945

DCT:	110092799 110114079 110114986

PEQ:	110019548 110025965

AOF:	109833124 109834320 109834432

MSIN:

NCOL:

ATR:

Reference

PMID:9490746

Authors

Choe S, Dilkes BP, Fujioka S, Takatsuto S, Sakurai A, Feldmann KA

Title

The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis.

Journal

Plant Cell 10:231-43 (1998)
DOI:10.1105/tpc.10.2.231

Sequence

[ath:AT3G50660]

Reference

PMID:16460510

Authors

Fujita S, Ohnishi T, Watanabe B, Yokota T, Takatsuto S, Fujioka S, Yoshida S, Sakata K, Mizutani M.

Title

Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols.

Journal

Plant J 45:765-74 (2006)
DOI:10.1111/j.1365-313X.2005.02639.x

Sequence

[ath:AT3G50660]

LinkDB

DBGET integrated database retrieval system