KEGG ORTHOLOGY: K12721

ORTHOLOGY: K12721

Entry

K12721            Tight     KO

Symbol

cloN3, couN3

Name

L-prolyl-[peptidyl-carrier protein] dehydrogenase [EC:1.3.8.14]

Pathway

map00401

Novobiocin biosynthesis

map01110

Biosynthesis of secondary metabolites

Reaction

R06747

L-prolyl-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12721  cloN3, couN3; L-prolyl-[peptidyl-carrier protein] dehydrogenase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.8  With a flavin as acceptor
    1.3.8.14  L-prolyl-[peptidyl-carrier protein] dehydrogenase
     K12721  cloN3, couN3; L-prolyl-[peptidyl-carrier protein] dehydrogenase

Other DBs

COG:

COG1960

Genes

AG:	AAG29788(couN3) AAN65232(cloN3)

Reference

PMID:12480894

Authors

Pojer F, Li SM, Heide L.

Title

Molecular cloning and sequence analysis of the clorobiocin biosynthetic gene cluster: new insights into the biosynthesis of aminocoumarin antibiotics.

Journal

Microbiology 148:3901-11 (2002)
DOI:10.1099/00221287-148-12-3901

Sequence

[ag:AAN65232]

Reference

PMID:11036020

Authors

Wang ZX, Li SM, Heide L

Title

Identification of the coumermycin A(1) biosynthetic gene cluster of Streptomyces rishiriensis DSM 40489.

Journal

Antimicrob Agents Chemother 44:3040-8 (2000)
DOI:10.1128/AAC.44.11.3040-3048.2000

Sequence

[ag:AAG29788]

LinkDB

ENZYME: 1.3.8.14

Entry

EC 1.3.8.14                 Enzyme

Name

L-prolyl-[peptidyl-carrier protein] dehydrogenase;
pigA (gene name);
bmp3 (gene name);
pltE (gene name);
redW (gene name);
(L-prolyl)-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With a flavin as acceptor

Sysname

L-prolyl-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase

Reaction(IUBMB)

L-prolyl-[peptidyl-carrier protein] + 2 electron-transfer flavoprotein = 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 2 reduced electron-transfer flavoprotein [RN:R06747]

Reaction(KEGG)

R06747

Substrate

L-prolyl-[peptidyl-carrier protein] [CPD:C12470];
electron-transfer flavoprotein [CPD:C04253]

Product

1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] [CPD:C12483];
reduced electron-transfer flavoprotein [CPD:C04570]

Comment

Contains FAD. The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It is believed to catalyse the formation of a Delta2-pyrrolin-2-carbonyl-[peptidyl-carrier protein] intermediate, followed by a two-electron oxidation to 1H-pyrrol-2-carbonyl-[peptidyl-carrier protein].

History

EC 1.3.8.14 created 2017

Pathway

ec00333

Prodigiosin biosynthesis

ec00401

Novobiocin biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12721

L-prolyl-[peptidyl-carrier protein] dehydrogenase

K21782

L-prolyl-PCP dehydrogenase

Genes

IZH:	FEM41_11915

SMW:	SMWW4_v1c11040(pigA)

SRR:	SerAS9_1753

SRS:	SerAS12_1753

SRA:	SerAS13_1754

SERF:

L085_23030

SERM:

CLM71_22610

SSUR:

ATE40_002235

SNEM:

NLX84_05505

SERA:

Ser39006_020695

SERQ:

CWC46_20700

PROD:

PCO85_04850

XHO:	A9255_04280

PMAG:

JI723_05675

VGA:	BSQ33_17325

VSR:	Vspart_03968(pigA)

VRU:	RND59_20195

PAG:	PLES_25861(pltE)

PAF:	PAM18_2390(pltE)

PAEL:

T223_13215

PFL:	PFL_2791(pltE)

PPRC:

PFLCHA0_c28490(mmgC4)

PPRO:

PPC_2836(pltE)

PPII:

QL104_13960(pltE)

PPHE:

PP2015_464

PRR:	AT705_06580

PTU:	PTUN_a2937

PXI:	J5O05_20760

PVB:	J5X90_18210

PSAZ:

PA25_35580

MMAI:

sS8_1540

HCH:

HCH_06021

HAHE:

ENC22_25940

HAHH:

O5O45_30065

HPRO:

LMS44_05385

MME:	Marme_4091

CARI:

FNU76_06120

CCUP:

BKK81_22635

BRH:	RBRH_01923 RBRH_03863

KIA:	G8A07_12225

PAQA:

K9V56_011385

RUG:	QC826_00990

EBA:

ebA4327

ABRE:

pbN1_28250(acdA2)

MOH:	IHQ72_27610

MOC:	BB934_08570

MIWA:

SS37A_37750

SIW:	GH266_22965

TSV:	DSM104635_00867(acdA_5)

RPEL:

N7U68_00740

PAED:

G5B38_20150

ACRO:

K3J57_15270

GEM:

GM21_0556

GEB:

GM18_3813

GUR:

Gura_2352

GBM:

Gbem_0543

GBN:	GEOBRER4_05480

GER:	KP004_01955

GSUB:

KP001_13535

GNT:	KP003_01590

GPL:	M1B72_19015

PEF:	A7E78_03240

DES:	DSOUD_2098

DMM:	dnm_038660

SAT:

SYN_01146

AORY:

AMOR_00670

MXA:

MXAN_4304

MSD:	MYSTI_05335 MYSTI_06017

MYM:	A176_002585

MFB:	MFUL124B02_24100

MYX:	QEG98_23020

MLAN:

JY572_36875 JY572_40525

MFU:	LILAB_29670

MMAS:

MYMAC_004222

SUR:	STAUR_4841

MBD:	MEBOL_001696 MEBOL_007008

CFUS:

CYFUS_005954

SAMY:

DB32_001324

LGY:	T479_05845

PAEQ:

R50912_17985

PLUT:

EI981_06705

ESS:	ATZ33_00115

CCB:	Clocel_2633

CSR:	Cspa_c03050(acdA)

CRW:	CROST_036530(acdA) CROST_042510(pigA)

CFB:	CLFE_005400(pigA) CLFE_011230(acdA)

CAUN:

CLAUR_012590(acdA) CLAUR_032520(pigA)

VGU:	HYG85_05660

VPY:	HZI73_06095

SEDZ:

JYG23_13425

NTP:	CRH09_26870

NOZ:	DMB37_03565

NOD:	FOH10_16490

NAH:	F5544_12765

SCO:	SCO5879(redW)

SGB:	WQO_24555 WQO_29010

SANL:

KZO11_19765

SHY:

SHJG_0588

SHO:	SHJGH_0421

SBH:	SBI_07709(fadE30)

SDV:	BN159_2475(pyr7)

SLV:	SLIV_09210

SPRI:

SPRI_0152 SPRI_0225 SPRI_7128 SPRI_7201

SCZ:	ABE83_19775

SGM:	GCM10017557_78270 GCM10017557_82740

STRD:

NI25_09835

SSIA:

A7J05_21380

SVU:	B1H20_15480

SPUN:

BFF78_29675

SLAU:

SLA_2867 SLA_7475

SALF:

SMD44_07473

STRO:

STRMOE7_30645

SRJ:

SRO_6332

SQZ:	FQU76_00515 FQU76_33225

SGZ:	C0216_31280

SRK:	FGW37_26865

SPAD:

DVK44_31035

SAQU:

EJC51_24885

SGF:	HEP81_06681

SCAV:

CVT27_14810

SSUB:

CP968_00515

STSU:

B7R87_00410

SSPB:

CP982_34510

SBRO:

GQF42_07510

SATA:

C5746_38465

SCYG:

S1361_04960(acdA1)

SFEU:

IM697_30995

SLIA:

HA039_01155

SVD:	CP969_03660

SCAL:

I6J39_15495

SXN:	IAG42_36785

SHK:	J2N69_01110

STRY:

EQG64_15535

SDD:	D9753_08585 D9753_11315 D9753_34405

SLF:	JEQ17_02480

SAOV:

G3H79_04160

STUB:

MMF93_27610

SCIR:

STRCI_006117

SDRZ:

NEH16_32305

SCOA:

QU709_09960

SROC:

RGF97_13740

SFP:	QUY26_05280

SARM:

DVA86_03340 DVA86_18290

SCAJ:

O1G22_20930

ABRY:

NYE86_17000

ARUB:

J5A65_13315

NDA:

Ndas_4720

NAL:

B005_4297

NGV:	CDO52_00490

NAKE:

KGD83_01555

STRR:

EKD16_15785(mmgC6)

AMAZ:

LUW76_14360

ACIR:

OG979_36035

NGN:	LCN96_24155

MHAI:

OHB01_08835

AMYB:

BKN51_28030

AMOG:

QRX60_49345

SESP:

BN6_24020

KPHY:

AOZ06_22105 AOZ06_42060

LED:	BBK82_35560

ALO:	CRK58154 CRK58857 CRK62141

CROS:

N8J89_24220

UME:	RM788_51330

MFEU:

H1D33_25750

ACTR:

Asp14428_17360

AOU:	ACTOB_006435

PSUU:

Psuf_018910

CATI:

CS0771_15590

DROS:

Drose_14255

NHY:	JQS43_11190

PPSU:

NO713_02809(pltE)

MPRO:

BJP34_30085

AWA:	AA650_19340

CSG:	Cylst_6223

CALO:

Cal7507_5042

BSEN:

DP114_33460

BOX:	DP113_33565

NCN:	BZZ01_10930

KBS:	EPA93_14715

KBB:	ccbrp13_70320

SMAM:

Mal15_54510(acdA)

PLH:	VT85_13010(acdA)

IPA:

Isop_1449

SACI:

Sinac_1586

PBOR:

BSF38_05639(acdA)

AGV:	OJF2_60380(acdA_2)

TPLA:

ElP_61390(acdA)

RSR:	T7867_16695

WIN:

WPG_0386

ALUT:

O5O44_10395

RLO:	GQ464_016185

» show all

Reference

1 [PMID:11880032]

Authors

Thomas MG, Burkart MD, Walsh CT.

Title

Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.

Journal

Chem Biol 9:171-84 (2002)
DOI:10.1016/S1074-5521(02)00100-X

Sequence

[pfl:PFL_2791] [sco:SCO5879]

Reference

2 [PMID:15528645]

Authors

Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP

Title

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.

Journal

Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0

Sequence

[ag:CAH55629]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.8.14

IUBMB Enzyme Nomenclature:

1.3.8.14

ExPASy - ENZYME nomenclature database:

1.3.8.14

BRENDA, the Enzyme Database:

1.3.8.14

LinkDB

REACTION: R06747

Entry

R06747                      Reaction

Name

L-prolyl-[peptidyl-carrier protein]:electron-transfer flavoprotein oxidoreductase

Definition

L-Prolyl-[pcp] + 2 Electron-transferring flavoprotein <=> Pyrrole-2-carbonyl-[pcp] + 2 Reduced electron-transferring flavoprotein

Equation

C12470 + 2 C04253 <=> C12483 + 2 C04570

Reaction class

RC03357

C12470_C12483

Enzyme

1.3.8.14

Pathway

rn00333

Prodigiosin biosynthesis

rn00401

Novobiocin biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00837

Prodigiosin biosynthesis, L-proline => prodigiosin

M00838

Undecylprodigiosin biosynthesis, L-proline => undecylprodigiosin

Brite

Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
  1.3  Acting on the CH-CH group of donors
   1.3.8  With a flavin as acceptor
    1.3.8.14
     R06747  L-Prolyl-[pcp] + 2 Electron-transferring flavoprotein <=> Pyrrole-2-carbonyl-[pcp] + 2 Reduced electron-transferring flavoprotein

Orthology

K12721

L-prolyl-[peptidyl-carrier protein] dehydrogenase [EC:1.3.8.14]

K21782

L-prolyl-PCP dehydrogenase [EC:1.3.8.14]

Other DBs

RHEA:

55155

LinkDB

DBGET integrated database retrieval system