KEGG   ORTHOLOGY: K12747
Entry
K12747            Tight     KO                                     
Symbol
cefG
Name
deacetylcephalosporin-C acetyltransferase [EC:2.3.1.175]
Pathway
map00311  Penicillin and cephalosporin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Reaction
R03064  acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K12747  cefG; deacetylcephalosporin-C acetyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.175  deacetylcephalosporin-C acetyltransferase
     K12747  cefG; deacetylcephalosporin-C acetyltransferase
Other DBs
GO: 0033813
Genes
AG: AAA32673(cefG)
Reference
PMID:1569032
  Authors
Gutierrez S, Velasco J, Fernandez FJ, Martin JF
  Title
The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase.
  Journal
J Bacteriol 174:3056-64 (1992)
DOI:10.1128/JB.174.9.3056-3064.1992
  Sequence
[ag:AAA32673]
LinkDB

KEGG   ENZYME: 2.3.1.175
Entry
EC 2.3.1.175                Enzyme                                 
Name
deacetylcephalosporin-C acetyltransferase;
acetyl-CoA:deacetylcephalosporin-C acetyltransferase;
DAC acetyltransferase;
cefG;
deacetylcephalosporin C acetyltransferase;
acetyl coenzyme A:DAC acetyltransferase;
acetyl-CoA:DAC acetyltransferase;
CPC acetylhydrolase;
acetyl-CoA:DAC O-acetyltransferase;
DAC-AT
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C [RN:R03064]
Reaction(KEGG)
R03064
Substrate
acetyl-CoA [CPD:C00024];
deacetylcephalosporin C [CPD:C03112]
Product
CoA [CPD:C00010];
cephalosporin C [CPD:C00916]
Comment
This enzyme catalyses the final step in the biosynthesis of cephalosporin C.
History
EC 2.3.1.175 created 2005
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12747  deacetylcephalosporin-C acetyltransferase
Reference
1  [PMID:1368946]
  Authors
Matsuyama K, Matsumoto H, Matsuda A, Sugiura H, Komatsu K, Ichikawa S.
  Title
Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum.
  Journal
Biosci Biotechnol Biochem 56:1410-2 (1992)
DOI:10.1271/bbb.56.1410
Reference
2  [PMID:1569032]
  Authors
Gutierrez S, Velasco J, Fernandez FJ, Martin JF
  Title
The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase.
  Journal
J Bacteriol 174:3056-64 (1992)
DOI:10.1128/JB.174.9.3056-3064.1992
  Sequence
[ag:AAA32673]
Reference
3  [PMID:1632779]
  Authors
Matsuda A, Sugiura H, Matsuyama K, Matsumoto H, Ichikawa S, Komatsu K
  Title
Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C o-acetyltransferase from Acremonium chrysogenum.
  Journal
Biochem Biophys Res Commun 186:40-6 (1992)
DOI:10.1016/S0006-291X(05)80772-7
  Sequence
[ag:AAA32673]
Reference
4  [PMID:9421924]
  Authors
Gutierrez S, Velasco J, Marcos AT, Fernandez FJ, Fierro F, Barredo JL, Diez B, Martin JF.
  Title
Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum.
  Journal
Appl Microbiol Biotechnol 48:606-14 (1997)
DOI:10.1007/s002530051103
Reference
5  [PMID:9895280]
  Authors
Velasco J, Gutierrez S, Campoy S, Martin JF.
  Title
Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits.
  Journal
Biochem J 337 ( Pt 3):379-85 (1999)
Reference
6  [PMID:7847890]
  Authors
Martin JF, Gutierrez S, Fernandez FJ, Velasco J, Fierro F, Marcos AT, Kosalkova K.
  Title
Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins.
  Journal
Antonie Van Leeuwenhoek 65:227-43 (1994)
DOI:10.1007/bf00871951
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.175
IUBMB Enzyme Nomenclature: 2.3.1.175
ExPASy - ENZYME nomenclature database: 2.3.1.175
BRENDA, the Enzyme Database: 2.3.1.175
CAS: 57827-76-6
LinkDB

KEGG   REACTION: R03064
Entry
R03064                      Reaction                               
Name
acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
Definition
Cephalosporin C + CoA <=> Deacetylcephalosporin C + Acetyl-CoA
Equation
C00916 + C00010 <=> C03112 + C00024
Reaction class
RC00004  C00010_C00024
RC00041  C00916_C03112
Enzyme
2.3.1.175
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01100  Metabolic pathways
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.175
     R03064  Cephalosporin C + CoA <=> Deacetylcephalosporin C + Acetyl-CoA
Orthology
K12747  deacetylcephalosporin-C acetyltransferase [EC:2.3.1.175]
Other DBs
RHEA: 23863
LinkDB

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