KEGG   ORTHOLOGY: K13037
Entry
K13037                      KO                                     
Symbol
bacD
Name
L-alanine-L-anticapsin ligase [EC:6.3.2.49]
Pathway
map00998  Biosynthesis of various antibiotics
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00787  Bacilysin biosynthesis, prephenate => bacilysin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K13037  bacD; L-alanine-L-anticapsin ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.49  L-alanine---L-anticapsin ligase
     K13037  bacD; L-alanine-L-anticapsin ligase
Other DBs
RN: R11064
COG: COG0439
GO: 0034026
Genes
BSU: BSU37710(bacD)
BSR: I33_3919(bacD)
BSL: A7A1_0190
BSH: BSU6051_37710(bacD)
BSY: I653_18500
BSUT: BSUB_04007(bacD)
BSUL: BSUA_04007(bacD)
BSUS: Q433_20770
BSO: BSNT_10429(ywfE)
BSN: BSn5_09845
BSQ: B657_37710(bacD)
BSX: C663_3676(bacD)
BSS: BSUW23_18625(bacD)
BST: GYO_4157(bacD)
BAQ: BACAU_3519(bacD)
BYA: BANAU_3672(bacD)
BAMP: B938_17910(bacD)
BQY: MUS_4149(bacD)
BAML: BAM5036_3420(bacD)
BAMA: RBAU_3628(bacD)
BAMN: BASU_3404(bacD)
BAMB: BAPNAU_3686(bacD)
BAMT: AJ82_19695
BAMY: V529_37620(bacD)
BMP: NG74_03665(bacD_2)
BAO: BAMF_3604(bacD)
BAZ: BAMTA208_19085(bacD)
BQL: LL3_03915(bacD)
BXH: BAXH7_03907(bacD)
BAMI: KSO_001610
BAMC: U471_36350
BAMF: U722_18645
BMOJ: HC660_36790(bacD)
BTEQ: G4P54_19445(bacD)
BPU: BPUM_3423
BPUM: BW16_18175
BPUS: UP12_17620
BJS: MY9_3862
BACW: QR42_17175
BACP: SB24_11245
BACB: OY17_01115
BACY: QF06_17295
BACL: BS34A_40890(bacD)
BALM: BsLM_3801
 » show all
Reference
  Authors
Tabata K, Ikeda H, Hashimoto S
  Title
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
  Journal
J Bacteriol 187:5195-202 (2005)
DOI:10.1128/JB.187.15.5195-5202.2005
  Sequence
[bsu:BSU37710]
Reference
  Authors
Ozcengiz G, Ogulur I
  Title
Biochemistry, genetics and regulation of bacilysin biosynthesis and its significance more than an antibiotic.
  Journal
N Biotechnol 32:612-9 (2015)
DOI:10.1016/j.nbt.2015.01.006
LinkDB

KEGG   ENZYME: 6.3.2.49
Entry
EC 6.3.2.49                 Enzyme                                 
Name
L-alanine---L-anticapsin ligase;
BacD;
alanine-anticapsin ligase;
L-Ala-L-anticapsin ligase;
ywfE (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
L-alanine:L-anticapsin ligase (ADP-forming)
Reaction(IUBMB)
ATP + L-alanine + L-anticapsin = ADP + phosphate + bacilysin [RN:R11064]
Reaction(KEGG)
R11064
Substrate
ATP [CPD:C00002];
L-alanine [CPD:C00041];
L-anticapsin [CPD:C20941]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
bacilysin [CPD:C20942]
Comment
The enzyme, characterized from the bacterium Bacillus subtilis, is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. The enzyme requires Mg2+ or Mn2+ for activity, and has a broad substrate specificity in vitro [1].
History
EC 6.3.2.49 created 2006 as EC 6.3.2.28, transferred 2015 to EC 6.3.2.49
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13037  L-alanine-L-anticapsin ligase
Genes
BSUBSU37710(bacD)
BSRI33_3919(bacD)
BSLA7A1_0190
BSHBSU6051_37710(bacD)
BSYI653_18500
BSUTBSUB_04007(bacD)
BSULBSUA_04007(bacD)
BSUSQ433_20770
BSOBSNT_10429(ywfE)
BSNBSn5_09845
BSQB657_37710(bacD)
BSXC663_3676(bacD)
BSPU712_19005
BSSBSUW23_18625(bacD)
BSTGYO_4157(bacD)
BAYRBAM_034900
BAQBACAU_3519(bacD)
BYABANAU_3672(bacD)
BAMPB938_17910(bacD)
BQYMUS_4149(bacD)
BAMLBAM5036_3420(bacD)
BAMARBAU_3628(bacD)
BAMNBASU_3404(bacD)
BAMBBAPNAU_3686(bacD)
BAMTAJ82_19695
BAMYV529_37620(bacD)
BMPNG74_03665(bacD_2)
BAOBAMF_3604(bacD)
BAZBAMTA208_19085(bacD)
BQLLL3_03915(bacD)
BXHBAXH7_03907(bacD)
BAMIKSO_001610
BAMCU471_36350
BAMFU722_18645
BSIACWD84_01675
BVMB9C48_17860
BHTDIC78_12120
BMOJHC660_36790(bacD)
BTEQG4P54_19445(bacD)
BIQAN935_19100
BPUBPUM_3423
BPUMBW16_18175
BPUSUP12_17620
BJSMY9_3862
BACWQR42_17175
BACPSB24_11245
BACBOY17_01115
BACYQF06_17295
BACLBS34A_40890(bacD)
BALMBsLM_3801
BALTCFN77_18080
BACSAUL54_12245
BSAFBSL056_18720
BITBIS30_08570
BGIBGM20_13115
BMURABE28_019130
PALRHGI30_05295
SSPBCP982_01625
 » show all
Reference
1  [PMID:16030213]
  Authors
Tabata K, Ikeda H, Hashimoto S
  Title
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
  Journal
J Bacteriol 187:5195-202 (2005)
DOI:10.1128/JB.187.15.5195-5202.2005
  Sequence
[bsu:BSU37710]
Reference
2  [PMID:22298000]
  Authors
Tsuda T, Suzuki T, Kojima S
  Title
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 68:203-6 (2012)
DOI:10.1107/S174430911105425X
Reference
3  [PMID:22407814]
  Authors
Shomura Y, Hinokuchi E, Ikeda H, Senoo A, Takahashi Y, Saito J, Komori H, Shibata N, Yonetani Y, Higuchi Y
  Title
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.
  Journal
Protein Sci 21:707-16 (2012)
DOI:10.1002/pro.2058
  Sequence
[bsu:BSU37710]
Reference
4  [PMID:23317005]
  Authors
Parker JB, Walsh CT
  Title
Action and timing of BacC and BacD in the late stages of biosynthesis of the dipeptide antibiotic bacilysin.
  Journal
Biochemistry 52:889-901 (2013)
DOI:10.1021/bi3016229
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.49
IUBMB Enzyme Nomenclature: 6.3.2.49
ExPASy - ENZYME nomenclature database: 6.3.2.49
BRENDA, the Enzyme Database: 6.3.2.49
LinkDB

KEGG   REACTION: R11064
Entry
R11064                      Reaction                               
Name
L-alanine:L-anticapsin ligase (ADP-forming)
Definition
L-Anticapsin + L-Alanine + ATP <=> Bacilysin + Orthophosphate + ADP
Equation
Reaction class
RC00002  C00002_C00008
RC00064  C20941_C20942
RC00141  C00041_C20942
Enzyme
Pathway
rn00998  Biosynthesis of various antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00787  Bacilysin biosynthesis, prephenate => bacilysin
Orthology
K13037  L-alanine-L-anticapsin ligase [EC:6.3.2.49]
Other DBs
RHEA: 44335
LinkDB

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