KEGG   ORTHOLOGY: K13258
Entry
K13258                      KO                                     
Symbol
HIDH
Name
2-hydroxyisoflavanone dehydratase [EC:4.2.1.105]
Pathway
map00943  Isoflavonoid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00941  Isoflavone biosynthesis, liquiritigenin/naringenin => daidzein/genistein
Reaction
R06793  2-hydroxy-2,3-dihydrogenistein hydro-lyase (genistein-forming)
R07317  2,7,4'-trihydroxyisoflavanone hydro-lyase (daidzein-forming)
R07723  2,7-dihydroxy-4'-methoxyisoflavanone hydro-lyase (formononetin-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00943 Isoflavonoid biosynthesis
    K13258  HIDH; 2-hydroxyisoflavanone dehydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.105  2-hydroxyisoflavanone dehydratase
     K13258  HIDH; 2-hydroxyisoflavanone dehydratase
Other DBs
GO: 0033987
Genes
GMX: 100785409 547489(HIDH)
GSJ: 114367895 114371837
PVU: PHAVU_002G047500g PHAVU_007G054200g
VRA: 106776973
VAR: 108319923
VUN: 114166941 114190838
VUM: 124848192
CCAJ: 109791254 109794824 109801193 109801222
APRC: 113870130 113871120
MTR: 11413175 11437326
TPRA: 123882884 123909768
CAM: 101502005 101506056
PSAT: 127076827 127097146
VVO: 131611419
ADU: 107466945
AIP: 107617879
AHF: 112711824 112776333
LANG: 109331604
QLO: 115994703
APAN: 127266629
 » show all
Reference
PMID:15734910
  Authors
Akashi T, Aoki T, Ayabe S
  Title
Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis.
  Journal
Plant Physiol 137:882-91 (2005)
DOI:10.1104/pp.104.056747
  Sequence
[gmx:547489]
LinkDB

KEGG   ENZYME: 4.2.1.105
Entry
EC 4.2.1.105                Enzyme                                 
Name
2-hydroxyisoflavanone dehydratase;
2,7,4'-trihydroxyisoflavanone hydro-lyase;
2,7,4'-trihydroxyisoflavanone hydro-lyase (daidzein-forming)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
2,4',7-trihydroxyisoflavanone hydro-lyase (daidzein-forming)
Reaction(IUBMB)
(1) 2,4',7-trihydroxyisoflavanone = daidzein + H2O [RN:R07317];
(2) 2,4',5,7-tetrahydroxyisoflavanone = genistein + H2O
Reaction(KEGG)
R07317;
(other) R06793 R07723
Substrate
2,4',7-trihydroxyisoflavanone [CPD:C15567];
2,4',5,7-tetrahydroxyisoflavanone [CPD:C12631]
Product
daidzein [CPD:C10208];
H2O [CPD:C00001];
genistein [CPD:C06563]
Comment
Catalyses the final step in the formation of the isoflavonoid skeleton. The reaction also occurs spontaneously.
History
EC 4.2.1.105 created 2004, modified 2013
Pathway
ec00943  Isoflavonoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13258  2-hydroxyisoflavanone dehydratase
Genes
GMX: 100785409 547489(HIDH)
GSJ: 114367895 114371837
PVU: PHAVU_002G047500g PHAVU_007G054200g
VRA: 106776973
VAR: 108319923
VUN: 114166941 114190838
VUM: 124848192
CCAJ: 109791254 109794824 109801193 109801222
APRC: 113870130 113871120
MTR: 11413175 11437326
TPRA: 123882884 123909768
CAM: 101502005 101506056
PSAT: 127076827 127097146
VVO: 131611419
ADU: 107466945
AIP: 107617879
AHF: 112711824 112776333
LANG: 109331604
QLO: 115994703
APAN: 127266629
 » show all
Reference
1
  Authors
Hakamatsuka, T., Mori, K., Ishida, S., Ebizuka, Y and Sankawa, U.
  Title
Purification of 2-hydroxyisoflavanone dehydratase from the cell cultures of Pueraria lobata.
  Journal
Phytochemistry 49:497-505 (1998)
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.105
IUBMB Enzyme Nomenclature: 4.2.1.105
ExPASy - ENZYME nomenclature database: 4.2.1.105
BRENDA, the Enzyme Database: 4.2.1.105
CAS: 166800-10-8
LinkDB

KEGG   REACTION: R06793
Entry
R06793                      Reaction                               
Name
2-hydroxy-2,3-dihydrogenistein hydro-lyase (genistein-forming)
Definition
2-Hydroxy-2,3-dihydrogenistein <=> Genistein + H2O
Equation
C12631 <=> C06563 + C00001
Comment
2-hydroxyisoflavanone dehydratase
Reaction class
RC01632  C06563_C12631
Enzyme
4.2.1.105
Pathway
rn00943  Isoflavonoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00941  Isoflavone biosynthesis, liquiritigenin/naringenin => daidzein/genistein
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.105
     R06793  2-Hydroxy-2,3-dihydrogenistein <=> Genistein + H2O
Orthology
K13258  2-hydroxyisoflavanone dehydratase [EC:4.2.1.105]
Other DBs
RHEA: 36806
LinkDB

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