KEGG   ORTHOLOGY: K13560
Entry
K13560            Tight     KO                                     
Symbol
btrK
Name
L-glutamyl-[BtrI acyl-carrier protein] decarboxylase [EC:4.1.1.95]
Pathway
map00524  Neomycin, kanamycin and gentamicin biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R08907  gamma-L-glutamyl-[acp] carboxy-lyase (4-aminobutyryl-[acp]-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00524 Neomycin, kanamycin and gentamicin biosynthesis
    K13560  btrK; L-glutamyl-[BtrI acyl-carrier protein] decarboxylase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.1  Carboxy-lyases
    4.1.1.95  L-glutamyl-[BtrI acyl-carrier protein] decarboxylase
     K13560  btrK; L-glutamyl-[BtrI acyl-carrier protein] decarboxylase
Genes
AG: BAE07075(btrK)
Reference
PMID:16156513
  Authors
Kudo F, Numakura M, Tamegai H, Yamamoto H, Eguchi T, Kakinuma K
  Title
Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.
  Journal
J Antibiot (Tokyo) 58:373-9 (2005)
DOI:10.1038/ja.2005.47
  Sequence
[ag:BAE07075]
Reference
PMID:19362652
  Authors
Kudo F, Eguchi T
  Title
Biosynthetic enzymes for the aminoglycosides butirosin and neomycin.
  Journal
Methods Enzymol 459:493-519 (2009)
DOI:10.1016/S0076-6879(09)04620-5
Reference
PMID:15975512
  Authors
Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB
  Title
Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.
  Journal
Chem Biol 12:665-75 (2005)
DOI:10.1016/j.chembiol.2005.04.010
  Sequence
[ag:BAE07075]
LinkDB

KEGG   ENZYME: 4.1.1.95
Entry
EC 4.1.1.95                 Enzyme                                 
Name
L-glutamyl-[BtrI acyl-carrier protein] decarboxylase;
btrK (gene name)
Class
Lyases;
Carbon-carbon lyases;
Carboxy-lyases
Sysname
L-glutamyl-[BtrI acyl-carrier protein] carboxy-lyase
Reaction(IUBMB)
L-glutamyl-[BtrI acyl-carrier protein] = 4-amino butanoyl-[BtrI acyl-carrier protein] + CO2 [RN:R08907]
Reaction(KEGG)
R08907
Substrate
L-glutamyl-[BtrI acyl-carrier protein]
Product
4-amino butanoyl-[BtrI acyl-carrier protein];
CO2 [CPD:C00011]
Comment
Binds pyridoxal 5'-phosphate. Catalyses a step in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. Has very low activity with substrates not bound to an acyl-carrier protein.
History
EC 4.1.1.95 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13560  L-glutamyl-[BtrI acyl-carrier protein] decarboxylase
Reference
1  [PMID:15975512]
  Authors
Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB
  Title
Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.
  Journal
Chem Biol 12:665-75 (2005)
DOI:10.1016/j.chembiol.2005.04.010
  Sequence
[ag:BAE07075]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.1.95
IUBMB Enzyme Nomenclature: 4.1.1.95
ExPASy - ENZYME nomenclature database: 4.1.1.95
BRENDA, the Enzyme Database: 4.1.1.95
LinkDB

KEGG   REACTION: R08907
Entry
R08907                      Reaction                               
Name
gamma-L-glutamyl-[acp] carboxy-lyase (4-aminobutyryl-[acp]-forming)
Definition
gamma-L-Glutamyl-[acp] <=> 4-Aminobutyryl-[acp] + CO2
Equation
C18006 <=> C18007 + C00011
Comment
BtrI is an acyl-carrier protein.
Reaction class
RC00299  C18006_C18007
Enzyme
4.1.1.95
Pathway
rn00524  Neomycin, kanamycin and gentamicin biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.1  Carbon-carbon lyases
   4.1.1  Carboxy-lyases
    4.1.1.95
     R08907  gamma-L-Glutamyl-[acp] <=> 4-Aminobutyryl-[acp] + CO2
Orthology
K13560  L-glutamyl-[BtrI acyl-carrier protein] decarboxylase [EC:4.1.1.95]
Other DBs
RHEA: 53959
LinkDB

DBGET integrated database retrieval system