KEGG   ORTHOLOGY: K18091
Entry
K18091                      KO                                     
Symbol
pntE, penE, ptlE
Name
pentalenolactone D synthase [EC:1.14.13.170 1.14.13.171]
Pathway
map00998  Biosynthesis of various antibiotics
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Reaction
R10154  1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (pentalenolactone-D forming)
R10155  1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (neopentalenolactone-D forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K18091  pntE, penE, ptlE; pentalenolactone D synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.170  pentalenolactone D synthase
     K18091  pntE, penE, ptlE; pentalenolactone D synthase
    1.14.13.171  neopentalenolactone D synthase
     K18091  pntE, penE, ptlE; pentalenolactone D synthase
Other DBs
COG: COG2072
Genes
SPOG: EEB18_015800
SPJJ: V6U71_06165
MHOL: K3U96_25175
MYQ: TUM20985_33720
SMA: SAVERM_2994(ptlE)
SBH: SBI_09676
SCI: B446_29680
SCW: TU94_27390
SLE: sle_13190(sle_13190)
SSIA: A7J05_09255
SGJ: IAG43_27140
SHK: J2N69_35820
AG: ADO85575(pntE) ADO85591(penE)
 » show all
Reference
PMID:21250661
  Authors
Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE
  Title
Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone.
  Journal
Biochemistry 50:1739-54 (2011)
DOI:10.1021/bi1019786
  Sequence
[sma:SAVERM_2994] [ag:ADO85591 ADO85575]
LinkDB

KEGG   ENZYME: 1.14.13.171
Entry
EC 1.14.13.171              Enzyme                                 
Name
neopentalenolactone D synthase;
ptlE (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (neopentalenolactone-D-forming)
Reaction(IUBMB)
1-deoxy-11-oxopentalenate + NADPH + H+ + O2 = neopentalenolactone D + NADP+ + H2O [RN:R10155]
Reaction(KEGG)
R10155
Substrate
1-deoxy-11-oxopentalenate [CPD:C20400];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
neopentalenolactone D [CPD:C20402];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A FAD-dependent oxygenase. Isolated from the bacterium Streptomyces avermitilis. The ketone undergoes a biological Baeyer-Villiger reaction.
History
EC 1.14.13.171 created 2012
Pathway
ec00998  Biosynthesis of various antibiotics
ec01110  Biosynthesis of secondary metabolites
Orthology
K18091  pentalenolactone D synthase
Genes
SPOG: EEB18_015800
SPJJ: V6U71_06165
MHOL: K3U96_25175
MYQ: TUM20985_33720
SMA: SAVERM_2994(ptlE)
SBH: SBI_09676
SCI: B446_29680
SCW: TU94_27390
SLE: sle_13190(sle_13190)
SSIA: A7J05_09255
SGJ: IAG43_27140
SHK: J2N69_35820
 » show all
Reference
1  [PMID:21250661]
  Authors
Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE
  Title
Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone.
  Journal
Biochemistry 50:1739-54 (2011)
DOI:10.1021/bi1019786
  Sequence
[sma:SAVERM_2994]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.171
IUBMB Enzyme Nomenclature: 1.14.13.171
ExPASy - ENZYME nomenclature database: 1.14.13.171
BRENDA, the Enzyme Database: 1.14.13.171
LinkDB

KEGG   REACTION: R10155
Entry
R10155                      Reaction                               
Name
1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (neopentalenolactone-D forming)
Definition
1-Deoxy-11-oxopentalenate + NADPH + H+ + Oxygen <=> Neopentalenolactone D + NADP+ + H2O
Equation
C20400 + C00005 + C00080 + C00007 <=> C20402 + C00006 + C00001
Reaction class
RC00001  C00005_C00006
RC03066  C20400_C20402
Enzyme
1.14.13.171
Pathway
rn00998  Biosynthesis of various antibiotics
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.171
     R10155  1-Deoxy-11-oxopentalenate + NADPH + H+ + Oxygen <=> Neopentalenolactone D + NADP+ + H2O
Orthology
K18091  pentalenolactone D synthase [EC:1.14.13.170 1.14.13.171]
Other DBs
RHEA: 34642
LinkDB

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