KEGG   ORTHOLOGY: K22434
Entry
K22434            Tight     KO                                     
Symbol
PSIH
Name
tryptamine 4-monooxygenase [EC:1.14.99.59]
Pathway
map00901  Indole alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00962  Psilocybin biosynthesis, tryptophan => psilocybin
Reaction
R11912  tryptamine,hydrogen-donor:oxygen oxidoreductase (4-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K22434  PSIH; tryptamine 4-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.59  tryptamine 4-monooxygenase
     K22434  PSIH; tryptamine 4-monooxygenase
Other DBs
COG: COG2124
Genes
PCUB: JR316_0010786
AG: ASU62246(psiH)
Reference
PMID:28763571
  Authors
Fricke J, Blei F, Hoffmeister D
  Title
Enzymatic Synthesis of Psilocybin.
  Journal
Angew Chem Int Ed Engl 56:12352-12355 (2017)
DOI:10.1002/anie.201705489
  Sequence
[ag:ASU62246]
LinkDB

KEGG   ENZYME: 1.14.99.59
Entry
EC 1.14.99.59               Enzyme                                 
Name
tryptamine 4-monooxygenase;
PsiH
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
tryptamine,hydrogen-donor:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
tryptamine + reduced acceptor + O2 = 4-hydroxytryptamine + acceptor + H2O [RN:R11912]
Reaction(KEGG)
R11912
Substrate
tryptamine [CPD:C00398];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
4-hydroxytryptamine [CPD:C21762];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the fungus Psilocybe cubensis. Involved in the biosynthesis of the psychoactive compound psilocybin.
History
EC 1.14.99.59 created 2017
Pathway
ec00901  Indole alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22434  tryptamine 4-monooxygenase
Genes
PCUB: JR316_0010786
Reference
1  [PMID:28763571]
  Authors
Fricke J, Blei F, Hoffmeister D
  Title
Enzymatic Synthesis of Psilocybin.
  Journal
Angew Chem Int Ed Engl 56:12352-12355 (2017)
DOI:10.1002/anie.201705489
  Sequence
[ag:ASU62246]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.59
IUBMB Enzyme Nomenclature: 1.14.99.59
ExPASy - ENZYME nomenclature database: 1.14.99.59
BRENDA, the Enzyme Database: 1.14.99.59
LinkDB

KEGG   REACTION: R11912
Entry
R11912                      Reaction                               
Name
tryptamine,hydrogen-donor:oxygen oxidoreductase (4-hydroxylating)
Definition
Tryptamine + Reduced acceptor + Oxygen <=> 4-Hydroxytryptamine + Acceptor + H2O
Equation
C00398 + C00030 + C00007 <=> C21762 + C00028 + C00001
Reaction class
RC00046  C00398_C21762
Enzyme
1.14.99.59
Pathway
rn00901  Indole alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00962  Psilocybin biosynthesis, tryptophan => psilocybin
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.59
     R11912  Tryptamine + Reduced acceptor + Oxygen <=> 4-Hydroxytryptamine + Acceptor + H2O
Orthology
K22434  tryptamine 4-monooxygenase [EC:1.14.99.59]
Other DBs
RHEA: 15868
LinkDB

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