KEGG   ORTHOLOGY: K22946
Entry
K22946            Tight     KO                                     
Symbol
CPS4
Name
ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase [EC:4.2.1.173]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R11820  geranylgeranyl-diphosphate hydratase (ent-8alpha-hydroxylabd-13-en-15-yl-diphosphate-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K22946  CPS4; ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.173  ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase
     K22946  CPS4; ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase
Genes
SMIL: 130999236
AG: AKN91186(CPS4)
Reference
PMID:26077765
  Authors
Cui G, Duan L, Jin B, Qian J, Xue Z, Shen G, Snyder JH, Song J, Chen S, Huang L, Peters RJ, Qi X
  Title
Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza.
  Journal
Plant Physiol 169:1607-18 (2015)
DOI:10.1104/pp.15.00695
  Sequence
[ag:AKN91186]
LinkDB

KEGG   ENZYME: 4.2.1.173
Entry
EC 4.2.1.173                Enzyme                                 
Name
ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase;
SmCPS4
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
geranylgeranyl-diphosphate hydro-lyase (ent-8alpha-hydroxylabd-13-en-15-yl diphosphate-forming)
Reaction(IUBMB)
ent-8alpha-hydroxylabd-13-en-15-yl diphosphate = geranylgeranyl diphosphate + H2O [RN:R11820]
Reaction(KEGG)
R11820
Substrate
ent-8alpha-hydroxylabd-13-en-15-yl diphosphate [CPD:C21656]
Product
geranylgeranyl diphosphate [CPD:C00353];
H2O [CPD:C00001]
Comment
Isolated from the plant Salvia miltiorrhiza (red sage).
History
EC 4.2.1.173 created 2017
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22946  ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase
Genes
SMIL: 130999236
Reference
1  [PMID:26077765]
  Authors
Cui G, Duan L, Jin B, Qian J, Xue Z, Shen G, Snyder JH, Song J, Chen S, Huang L, Peters RJ, Qi X
  Title
Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza.
  Journal
Plant Physiol 169:1607-18 (2015)
DOI:10.1104/pp.15.00695
  Sequence
[ag:AKN91186]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.173
IUBMB Enzyme Nomenclature: 4.2.1.173
ExPASy - ENZYME nomenclature database: 4.2.1.173
BRENDA, the Enzyme Database: 4.2.1.173
LinkDB

KEGG   REACTION: R11820
Entry
R11820                      Reaction                               
Name
geranylgeranyl-diphosphate hydratase (ent-8alpha-hydroxylabd-13-en-15-yl-diphosphate-forming);
ent-8alpha-hydroxylabd-13-en-15-yl-diphosphate hydro-lyase
Definition
ent-8alpha-Hydroxylabd-13-en-15-yl diphosphate <=> Geranylgeranyl diphosphate + H2O
Equation
C21656 <=> C00353 + C00001
Reaction class
RC02988  C00353_C21656
Enzyme
4.2.1.173
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.173
     R11820  ent-8alpha-Hydroxylabd-13-en-15-yl diphosphate <=> Geranylgeranyl diphosphate + H2O
Orthology
K22946  ent-8alpha-hydroxylabd-13-en-15-yl diphosphate synthase [EC:4.2.1.173]
Other DBs
RHEA: 54651
LinkDB

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