KEGG ORTHOLOGY: K24666

ORTHOLOGY: K24666

Entry

K24666                      KO

Symbol

dsyB

Name

2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase [EC:2.1.1.373]

Pathway

map00920

Sulfur metabolism

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

Reaction

R12666

S-adenosyl-L-methionine:(2R)-2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K24666  dsyB; 2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.373  2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase
     K24666  dsyB; 2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase

Other DBs

COG:

COG2226

Genes

HOE:	IMCC20628_04415

RBM:

TEF_00930

BRN:	D1F64_17375

LAP:	ACP90_07270

LABR:

CHH27_12955

LABP:

FJ695_11515

LABT:

FIU93_12925(crtF)

LAGG:

B0E33_19335

RAGG:

QUB73_08235

SIW:	GH266_11265

YAN:	AYJ57_15890

TPRO:

Ga0080559_TMP4718

PABY:

Ga0080574_TMP997

RID:	RIdsm_02262(crtF_2)

AHT:	ANTHELSMS3_02971

SAGU:

CDO87_12755

SSTL:

ABFK29_04265

SAGZ:

QNI11_18680

OCD:	FHY55_10290

ROT:	FIV09_08250(crtF1)

MALU:

KU6B_20890

TGL:	HFZ77_02600

RED:	roselon_03022

RHC:

RGUI_3553

PPRU:

FDP22_08505

PALW:

PSAL_008650(aziB2)

PSHQ:

F3W81_04580

MANH:

LA6_002446(crtF)

NACI:

RANT:

SCAL:

Reference

PMID:28191900

Authors

Curson AR, Liu J, Bermejo Martinez A, Green RT, Chan Y, Carrion O, Williams BT, Zhang SH, Yang GP, Bulman Page PC, Zhang XH, Todd JD.

Title

Dimethylsulfoniopropionate biosynthesis in marine bacteria and identification of the key gene in this process.

Journal

Nat Microbiol 2:17009 (2017)
DOI:10.1038/nmicrobiol.2017.9

Sequence

[lagg:B0E33_19335]

LinkDB

ENZYME: 2.1.1.373

Entry

EC 2.1.1.373                Enzyme

Name

2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase;
dsyB (gene name);
methylthiohydroxybutyrate methyltransferase;
MTHB methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:(2R)-2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + (2R)-2-hydroxy-4-(methylsulfanyl)butanoate = S-adenosyl-L-homocysteine + (2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate [RN:R12666]

Reaction(KEGG)

R12666

Substrate

S-adenosyl-L-methionine [CPD:C00019];
(2R)-2-hydroxy-4-(methylsulfanyl)butanoate [CPD:C22315]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
(2R)-4-(dimethylsulfaniumyl)-2-hydroxybutanoate [CPD:C22316]

Comment

The enzyme, characterized from the marine bacterium Labrenzia aggregata, participates in the biosynthesis of dimethylsulfoniopropanoate (DMSP). A eukaryotic enzyme that shares little sequence similarity with the bacterial enzyme was identified in many marine phytoplankton species.

History

EC 2.1.1.373 created 2020

Pathway

ec00920

Sulfur metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K24666

2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase

Genes

HOE:	IMCC20628_04415

RBM:

TEF_00930

BRN:	D1F64_17375

LAP:	ACP90_07270

LABR:

CHH27_12955

LABP:

FJ695_11515

LABT:

FIU93_12925(crtF)

LAGG:

B0E33_19335

RAGG:

QUB73_08235

SIW:	GH266_11265

YAN:	AYJ57_15890

TPRO:

Ga0080559_TMP4718

PABY:

Ga0080574_TMP997

RID:	RIdsm_02262(crtF_2)

AHT:	ANTHELSMS3_02971

SAGU:

CDO87_12755

SSTL:

ABFK29_04265

SAGZ:

QNI11_18680

OCD:	FHY55_10290

ROT:	FIV09_08250(crtF1)

MALU:

KU6B_20890

TGL:	HFZ77_02600

RED:	roselon_03022

RHC:

RGUI_3553

PPRU:

FDP22_08505

PALW:

PSAL_008650(aziB2)

PSHQ:

F3W81_04580

MANH:

LA6_002446(crtF)

NACI:

NUH88_05915

RANT:

RHODO2019_06505

SCAL:

I6J39_29690

» show all

Reference

Authors

Summers PS, Nolte KD, Cooper AJL, Borgeas H, Leustek T, Rhodes D, Hanson AD.

Title

Identification and stereospecificity of the first three enzymes of 3-dimethylsulfoniopropionate biosynthesis in a chlorophyte alga.

Journal

Plant Physiol 116:369-378 (1998)

Reference

2 [PMID:28191900]

Authors

Curson AR, Liu J, Bermejo Martinez A, Green RT, Chan Y, Carrion O, Williams BT, Zhang SH, Yang GP, Bulman Page PC, Zhang XH, Todd JD.

Title

Dimethylsulfoniopropionate biosynthesis in marine bacteria and identification of the key gene in this process.

Journal

Nat Microbiol 2:17009 (2017)
DOI:10.1038/nmicrobiol.2017.9

Sequence

[lagg:B0E33_19335]

Reference

3 [PMID:29574051]

Authors

Kageyama H, Tanaka Y, Shibata A, Waditee-Sirisattha R, Takabe T.

Title

Dimethylsulfoniopropionate biosynthesis in a diatom Thalassiosira pseudonana: Identification of a gene encoding MTHB-methyltransferase.

Journal

Arch Biochem Biophys 645:100-106 (2018)
DOI:10.1016/j.abb.2018.03.019

Sequence

[tps:THAPSDRAFT_269095]

Reference

4 [PMID:29483657]

Authors

Curson ARJ, Williams BT, Pinchbeck BJ, Sims LP, Martinez AB, Rivera PPL, Kumaresan D, Mercade E, Spurgin LG, Carrion O, Moxon S, Cattolico RA, Kuzhiumparambil U, Guagliardo P, Clode PL, Raina JB, Todd JD.

Title

DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton.

Journal

Nat Microbiol 3:430-439 (2018)
DOI:10.1038/s41564-018-0119-5

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.373

IUBMB Enzyme Nomenclature:

2.1.1.373

ExPASy - ENZYME nomenclature database:

2.1.1.373

BRENDA, the Enzyme Database:

2.1.1.373

LinkDB

REACTION: R12666

Entry

R12666                      Reaction

Name

S-adenosyl-L-methionine:(2R)-2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase

Definition

S-Adenosyl-L-methionine + (2R)-2-Hydroxy-4-(methylsulfanyl)butanoate <=> S-Adenosyl-L-homocysteine + (2R)-4-(Dimethylsulfaniumyl)-2-hydroxybutanoate

Equation

C00019 + C22315 <=> C00021 + C22316

Reaction class

RC00347

C22315_C22316

Enzyme

2.1.1.373

Pathway

rn00920

Sulfur metabolism

rn01100

Metabolic pathways

rn01120

Microbial metabolism in diverse environments

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.373
     R12666  S-Adenosyl-L-methionine + (2R)-2-Hydroxy-4-(methylsulfanyl)butanoate <=> S-Adenosyl-L-homocysteine + (2R)-4-(Dimethylsulfaniumyl)-2-hydroxybutanoate

Orthology

K24666

2-hydroxy-4-(methylsulfanyl)butanoate S-methyltransferase [EC:2.1.1.373]

K28065

MTHB methyltransferase / DMSHB decarboxylase

LinkDB

DBGET integrated database retrieval system