KEGG   ORTHOLOGY: K25598
Entry
K25598                      KO                                     
Symbol
creM
Name
3-amino-2-hydroxy-4-methoxybenzoate diazotase [EC:6.7.1.1]
Pathway
map00998  Biosynthesis of various antibiotics
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00951  Cremeomycin biosynthesis, aspartate/3,4-AHBA => cremeomycin
Reaction
R12943  3-amino-2-hydroxy-4-methoxybenzoate:nitrite ligase (AMP-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K25598  creM; 3-amino-2-hydroxy-4-methoxybenzoate diazotase
Enzymes [BR:ko01000]
 6. Ligases
  6.7  Forming nitrogen-nitrogen bonds
   6.7.1  Forming diazo bonds
    6.7.1.1  3-amino-2-hydroxy-4-methoxybenzoate diazotase
     K25598  creM; 3-amino-2-hydroxy-4-methoxybenzoate diazotase
Genes
AG: ALA99210(creM)
Reference
PMID:26278892
  Authors
Waldman AJ, Pechersky Y, Wang P, Wang JX, Balskus EP.
  Title
The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation.
  Journal
Chembiochem 16:2172-5 (2015)
DOI:10.1002/cbic.201500407
  Sequence
[ag:ALA99210]
LinkDB

KEGG   ENZYME: 6.7.1.1
Entry
EC 6.7.1.1                  Enzyme                                 
Name
3-amino-2-hydroxy-4-methoxybenzoate diazotase;
creM (gene name)
Class
Ligases;
Forming nitrogen-nitrogen bonds;
Forming diazo bonds
Sysname
3-amino-2-hydroxy-4-methoxybenzoate:nitrite ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite = AMP + diphosphate + cremeomycin + H2O [RN:R12943]
Reaction(KEGG)
R12943
Substrate
ATP [CPD:C00002];
3-amino-2-hydroxy-4-methoxybenzoate [CPD:C22425];
nitrite [CPD:C00088]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
cremeomycin [CPD:C22416];
H2O [CPD:C00001]
Comment
The enzyme, characterized from Streptomyces cremeus, catalyses the last step in the biosynthesis of the ortho-diazoquinone cremeomycin.
History
EC 6.7.1.1 created 2021
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K25598  3-amino-2-hydroxy-4-methoxybenzoate diazotase
Reference
1  [PMID:29771512]
  Authors
Waldman AJ, Balskus EP.
  Title
Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.
  Journal
J Org Chem 83:7539-7546 (2018)
DOI:10.1021/acs.joc.8b00367
Other DBs
ExplorEnz - The Enzyme Database: 6.7.1.1
IUBMB Enzyme Nomenclature: 6.7.1.1
ExPASy - ENZYME nomenclature database: 6.7.1.1
BRENDA, the Enzyme Database: 6.7.1.1
LinkDB

KEGG   REACTION: R12943
Entry
R12943                      Reaction                               
Name
3-amino-2-hydroxy-4-methoxybenzoate:nitrite ligase (AMP-forming)
Definition
ATP + 3-Amino-2-hydroxy-4-methoxybenzoate + Nitrite <=> AMP + Diphosphate + Cremeomycin + H2O
Equation
C00002 + C22425 + C00088 <=> C00020 + C00013 + C22416 + C00001
Enzyme
6.7.1.1
Pathway
rn00998  Biosynthesis of various antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00951  Cremeomycin biosynthesis, aspartate/3,4-AHBA => cremeomycin
Brite
Enzymatic reactions [BR:br08201]
 6. Ligase reactions
  6.7  Forming nitrogen-nitrogen bonds
   6.7.1  Forming diazo bonds
    6.7.1.1
     R12943  ATP + 3-Amino-2-hydroxy-4-methoxybenzoate + Nitrite <=> AMP + Diphosphate + Cremeomycin + H2O
Orthology
K25598  3-amino-2-hydroxy-4-methoxybenzoate diazotase [EC:6.7.1.1]
LinkDB

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